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In vitro study of dietary factors affecting the biohydrogenation shift from trans-11 to trans-10 fatty acids in the rumen of dairy cows

Published online by Cambridge University Press:  29 September 2011

A. Zened ,
F. Enjalbert ,
M. C. Nicot  and
A. Troegeler-Meynadier
A. Zened
Affiliation:
INRA, UMR1289 Tandem, F-31326 Castanet-Tolosan, France Université de Toulouse, INP, ENSAT, ENVT, UMR1289 Tandem, F-31076 Toulouse, France
F. Enjalbert
Affiliation:
INRA, UMR1289 Tandem, F-31326 Castanet-Tolosan, France Université de Toulouse, INP, ENSAT, ENVT, UMR1289 Tandem, F-31076 Toulouse, France
M. C. Nicot
Affiliation:
INRA, UMR1289 Tandem, F-31326 Castanet-Tolosan, France Université de Toulouse, INP, ENSAT, ENVT, UMR1289 Tandem, F-31076 Toulouse, France
A. Troegeler-Meynadier*
Affiliation:
INRA, UMR1289 Tandem, F-31326 Castanet-Tolosan, France Université de Toulouse, INP, ENSAT, ENVT, UMR1289 Tandem, F-31076 Toulouse, France
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Abstract

On the basis of the isomer-specific effects of trans fatty acids (FA) on human health, and the detrimental effect of t10,c12-conjugated linoleic acid (CLA) on cows’ milk fat production, there is a need to identify factors that affect the shift from trans-11 to trans-10 pathway during ruminal biohydrogenation of FA. This experiment was conducted in vitro and aimed at separating the effects of the diet of the donor cows from those of the fermentative substrate, which is necessary to prevent this shift. A total of four dry Holstein dairy cows were used in a 4 × 4 Latin square design. They received 12 kg of dry matter per day of four diets based on maize silage during four successive periods: the control diet (22% starch, <3% fat); the high-starch diet, supplemented with wheat plus barley (35% starch, <3% crude fat); the sunflower oil diet, supplemented with 5% of sunflower oil (20% starch, 7.6% crude fat); and the high-starch plus oil diet (33% starch, 7.3% crude fat). Ruminal fluid of each donor cow was incubated for 5 h with four substrates having similar chemical composition to the diets, replacing sunflower oil by pure linoleic acid (LA). The efficiency of isomerisation of LA to CLA was the highest when rumen fluids from cows receiving dietary oil were incubated with added LA. The shift from trans-11 to trans-10 isomers was induced in vitro by high-starch diets and the addition of LA. Oil supplementation to the diet of the donor cows increased this shift. Conversely, the trans-10 isomer balance was always low when no LA was added to incubation cultures. These results showed that a large accumulation of trans-10 FA was only observed with an adapted microflora, as well as an addition of non-esterified LA to the incubation substrate.

Keywords

rumen biohydrogenationlinoleic acidconjugated linoleic acidstrans fatty acidstrans-10 shift
Type
Full Paper
Information
animal , Volume 6 , Issue 3: 61th EAAP Annual Meeting, 2010, Heraklion, Greece: 10th International Workshop on Biology of Lactation in Farm Animals (BOLFA) and Session “Evolution of mammary gland and milk secretion: consequences for lactation in farm animals” , 27 January 2012 , pp. 459 - 467
Copyright
Copyright © The Animal Consortium 2011

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