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High dietary vitamin A interferes with tissue α-tocopherol concentrations in fattening pigs: a study that examines administration and withdrawal times

Published online by Cambridge University Press:  29 May 2009

A. Olivares
Affiliation:
Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
A. I. Rey*
Affiliation:
Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
A. Daza
Affiliation:
Departamento de Producción Animal, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica, 28040 Madrid, Spain
C. J. Lopez-Bote
Affiliation:
Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
*
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Abstract

This study aimed to assess the interaction between different dietary vitamin A (dVitA) levels and the same concentration of vitamin E (100 IU all-rac-α-tocopheryl acetate/kg feed) in growing-finishing pigs. In the first experiment, two fat sources × two dVitA levels (0 v. 100 000 IU) were used. The supplementation of 100 000 IU dVitA induced a range of 5.13 to 30.03 μg retinol/g liver, 62.78 to 426.88 μg retinol palmitate/g liver, and 0.60 to 1.96 μg retinol/g fat. Dietary fat did not affect retinol or retinyl palmitate deposition in pigs. The high concentration of dVitA produced lower fat and liver α-tocopherol concentrations, and increased susceptibility of muscle tissue to oxidation. A second experiment was carried out to study the retinol and α-tocopherol retention at different withdrawal times prior to slaughter (two dVitA levels; 0 v. 100 000 IU). A high dose of 100 000 IU vitamin A during a short 2-week period was enough to induce α-tocopherol depletion in liver and fat to a similar extent as when 100 000 IU were administered during the whole fattening. Muscle, fat and liver α-tocopherol concentrations were not affected by dVitA in the 1300–13 000 IU/kg range, but liver α-tocopherol concentration was higher when vitamin A was removed from the vitamin mix 5 weeks prior to slaughter (experiment 3).

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Full Paper
Copyright
Copyright © The Animal Consortium 2009

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References

Abawi, FG, Sullivan, TW 1989. Interactions of vitamin A, vitamin D3, vitamin E and vitamin K in the diet of broiler chicks. Poultry Science 68, 14901498.Google Scholar
Abawi, FG, Sullivan, TW, Scheideler, SE 1985. Interaction of dietary fat with levels of vitamin-A and vitamin-E in broiler chicks. Poultry Science 64, 11921198.Google Scholar
Akhtar, MH, Bryan, M 2008. Extraction and quantification of major carotenoids in processed foods and supplements by liquid chromatography. Food Chemistry 111, 255261.Google Scholar
Alarcón-Corredor, OM, Alfonso, R 2007. Clinical and biochemical alterations in rats treated with high doses of vitamin A. Archivos Latinoamericanos de Nutrición 57, 224230.Google ScholarPubMed
Anderson, LE, Myer, RO, Brendemuhl, JH, Mcdowel, LR 1995. The effect of excessive dietary vitamin A on performance and vitamin E status in swine fed diets varying in dietary vitamin E. Journal of Animal Science 73, 10931098.CrossRefGoogle ScholarPubMed
Association of Official Analytical Chemists 2005. Official methods of analysis, 18th edition. AOAC, Washington, DC, USA.Google Scholar
Bartov, I, Sklan, D, Friedman, A 1997. Effect of vitamin A on the oxidative stability of broiler meat during storage: lack of interactions with vitamin E. British Poultry Science 38, 255257.Google Scholar
Blakely, SR, Mitchell, GV, Jenkins, MY, Grundele, E, Whittaker, P 1991. Canthaxanthin and excess vitamin A alter alpha-tocopherol, carotenoid and iron status in adult-rats. Journal of Nutrition 121, 16491655.CrossRefGoogle ScholarPubMed
Bradford, MM 1976. Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein–dye binding. Analytical Biochemistry 72, 248254.Google Scholar
Buckley, DJ, Morrissey, PA, Gray, JI 1995. Influence of dietary vitamin E on the oxidative stability and quality of pig meat. Journal of Animal Science 73, 31223130.Google Scholar
Butriss, JL, Diplock, AT 1984. High-performance liquid chromatography methods for vitamin E in tissues. Methods in Enzymology 105, 131138.Google Scholar
Ching, S, Mahan, DC, Wiseman, TG, Fastinger, ND 2002. Evaluating the antioxidant status of weanling pigs fed dietary vitamins A and E. Journal of Animal Science 80, 23962401.Google ScholarPubMed
Chung, YK, Mahan, DC, Lepine, AJ 1992. Efficacy of dietary d-alpha-tocopherol and dl-alpha-tocopheryl acetate for weanling pigs. Journal of Animal Science 70, 24852492.Google Scholar
D’Souza, DN, Pethick, DW, Dunshea, FR, Pluske, JR, Mullan, BP 2003. Nutritional manipulation increases intramuscular fat levels in the Longissimus muscle of female finisher pigs. Australian Journal of Agricultural Research 54, 745749.CrossRefGoogle Scholar
Drott, P, Ewald, U, Meurling, S 1993. Plasma levels of fat-soluble vitamin A and vitamin E in neonates, after administration of two different vitamin solutions. Clinical Nutrition 12, 96102.Google Scholar
Fraga, MJ, Villamide, MJ 2000. The composition of vitamin supplements in Spanish pig diets. Pigs News and Information 21, 6772.Google Scholar
Hogberg, A, Pickova, J, Babol, J, Andersson, K, Dutta, PC 2002. Muscle lipids, vitamins E and A, and lipid oxidation as affected by diet and RN genotype in female and castrated male Hampshire crossbreed pigs. Meat Science 60, 411420.Google Scholar
Hoppe, PP, Schoner, FJ, Frigg, M 1992. Effects of dietary retinol on hepatic retinol storage and on plasma and tissue alpha-tocopherol in pigs. International Journal for Vitamin and Nutrition Research 62, 121129.Google Scholar
Hosotani, K, Kitagawa, M 2005. Effects of dietary protein, fat and beta-carotene levels on beta-carotene absorption in rats. International Journal for Vitamin and Nutrition Research 75, 274280.Google Scholar
Kornbrust, DJ, Mavis, RD 1980. Relative susceptibility of microsomes from lung, heart, liver, kidney, brain, and testes to lipid-peroxidation: correlation with vitamin E content. Lipids 15, 315322.CrossRefGoogle ScholarPubMed
Lopez-Bote, CJ, Isabel, B, Ruiz, J, Daza, A 2003. Effect of vitamin E supplementation and partial substitution of poly-with mono-unsaturated fatty acids in pigs diets on muscle, and microsome extract α-tocopherol concentration and lipid oxidation. Archives of Animal Nutrition 57, 1125.Google Scholar
López-Bote, CJ, Rey, A, Ruiz, J, Isabel, B, Sanz-Arias, R 1997. Effect of feeding diets high in monounsaturated fatty acids and α-tocopheryl acetate to rabbits on resulting carcass fatty acid profile and lipid oxidation. Animal Science 64, 177186.CrossRefGoogle Scholar
Miller, CV, Waters, KM, Ntambi, JM 1997. Regulation of hepatic stearoyl-CoA desaturase gene 1 by vitamin A. Biochemical and Biophysical Research Communication 231, 206210.Google Scholar
Morrissey, PA, Buckley, DA, Galvin, K 2000. Vitamin E and the oxidative stability of pork and poultry. In Antioxidants in muscle foods: nutritional strategies to improve quality (ed. EA Decker, C Faustman and CJ López-Bote), pp. 263287. Wiley interscience, New York, USA.Google Scholar
Muhilal, H, Glover, J 1974. Effects of dietary deficiencies of protein and retinol on the plasma level of retinol-binding protein in rat. British Journal of Nutrition 32, 549558.Google Scholar
National Research Council 1998. Nutrient requirement of swine, 10th edition. NRC, National Academy Press, Washington, DC, USA.Google Scholar
Olivares, A, Daza, A, Rey, AI, Lopez-Bote, CJ 2009. Dietary vitamin A concentration alters fatty acid composition in pigs. Meat Science 81, 295299.Google Scholar
Pieszka, M 2003. Effect of vitamin A level in fat-added feed mixtures for broiler chickens on oxidative stability of meat lipids. Annals of Animal Science 3, 101108.Google Scholar
Pudelkiewicz, WJ, Webster, L, Matterson, LDS 1964. Effects of high levels of dietary vitamin A acetate on tissue tocopherol and some related analytical observations. Journal of Nutrition 84, 113117.CrossRefGoogle ScholarPubMed
Raghuramulu, N, Underwood, B, Bhaskaram, P, Arunjyothi, , Chennaiah, S, Reddy, V 1998. Vitamin A relative dose response test in undernourished children. Nutrition Research 18, 533541.Google Scholar
Rey, A, Daza, A, López-Carrasco, C, López-Bote, CJ 2006. Quantitative study of the alpha- and gamma-tocopherols accumulation in muscle and backfat from Iberian pigs kept free-range as affected by time of free-range feeding or weight gain. Animal Science 82, 901908.Google Scholar
Rey, A, Lopez-Bote, CJ 2001. Effect of dietary copper and vitamin E supplementation, and extensive feeding with acorns and grass on longissimus muscle composition and susceptibility to oxidation in Iberian pigs. Journal of Animal Physiology and Animal Nutrition 85, 281292.Google Scholar
Rey, AI, Lopez-Bote, CJ, Kerry, JP, Lynch, PB, Buckley, DJ, Morrissey, P 2001. Effects of dietary vegetable oil inclusion and composition on the susceptibility of pig meat to oxidation. Animal Science 72, 457463.Google Scholar
Siebert, BD, Kruck, ZA, Davis, J, Pitchford, WS, Harper, GS, Bottema, CDK 2006. Effect of low vitamin A status on fat deposition and fatty acid desaturation in beef cattle. Lipids 41, 365370.Google Scholar
Statistical Analysis System Institute (SAS) 1999. SAS user’s guide. SAS Institute Inc., Cary, NC, USA.Google Scholar
Sukhija, PS, Palmquist, DL 1988. Rapid method for determination of total fatty acid content and composition of feedstuffs and feces. Journal of Agricultural and Food Chemistry 36, 12021206.Google Scholar
Surai, PF, Kuklenko, TV 2000. Effects of vitamin A on the antioxidant systems of the growing chicken. Asian-Australasian Journal of Animal Science 13, 12901295.CrossRefGoogle Scholar
Surles, RL, Mills, JP, Valentine, AR, Tanumihardjo, SA 2007. One-time graded doses of vitamin A to weanling piglets enhance hepatic retinol but do not always prevent vitamin A deficiency. American Journal of Clinical Nutrition 86, 10451053.CrossRefGoogle Scholar
Tiftik, AM 1992. Conversion of carotenes into vitamin A and vitamin A metabolism. The mechanism of conversion of beta-carotene into vitamin A: a review. Veteriner Fakültesi Dergisi, Selcuk Üniversitesi 8, 35.Google Scholar
Villaverde, C, Baucells, MD, Manzanilla, EG, Barroeta, AC 2008. High levels of dietary unsaturated fat decrease alpha-tocopherol content of whole body, liver, and plasma of chickens without variations in intestinal apparent absorption. Poultry Science 87, 497505.CrossRefGoogle ScholarPubMed
Villaverde, C, Cortinas, L, Barroeta, AC, Martin-Orue, SM, Baucells, MD 2004. Relationship between dietary unsaturation and vitamin E in poultry. Journal of Animal Physiology and Animal Nutrition 88, 143149.Google Scholar