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Evolution of the fatty acid profile of subcutaneous back-fat adipose tissue in growing Iberian and Landrace × Large White pigs

Published online by Cambridge University Press:  02 October 2012

R. Barea ,
B. Isabel ,
R. Nieto ,
C. López-Bote  and
J. F. Aguilera
R. Barea
Affiliation:
Departamento de Fisiología y Bioquímica de la Nutrición Animal, Instituto de Nutrición Animal (INAN), Estación Experimental del Zaidín (CSIC), Cno. del Jueves s/n, 18100 Armilla, Granada, Spain
B. Isabel
Affiliation:
Producción Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain
R. Nieto
Affiliation:
Departamento de Fisiología y Bioquímica de la Nutrición Animal, Instituto de Nutrición Animal (INAN), Estación Experimental del Zaidín (CSIC), Cno. del Jueves s/n, 18100 Armilla, Granada, Spain
C. López-Bote
Affiliation:
Producción Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain
J. F. Aguilera*
Affiliation:
Departamento de Fisiología y Bioquímica de la Nutrición Animal, Instituto de Nutrición Animal (INAN), Estación Experimental del Zaidín (CSIC), Cno. del Jueves s/n, 18100 Armilla, Granada, Spain
*
†E-mail: [email protected]
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Abstract

The lipid content and fatty acid (FA) profile in pig tissues are strongly influenced by genotype and nutrient supply, with implications in meat quality. The de novo lipid synthesis and pattern of FA unsaturation could be an important cause of variation in the overall efficiency of energy utilization among breeds. To test the effects of pig genotype and CP supply on the evolution of back-fat tissue FA profile throughout the growing and finishing stages, 32 Iberian (IB) and Landrace × Large White (LR × LW) barrows were offered one of two diets differing in CP content (13% or 17% as fed). A pair-fed procedure (0.8 × ad libitum intake of IB pigs) was used. Subcutaneous fat samples were taken at the dorso-lumbar region at ∼38, 50, 65, 90 and 115 kg BW. Higher proportions of total monounsaturated FA (MUFA; P < 0.01) and lower proportions of total saturated FA (SFA; P < 0.01 to 0.05) were found in the outer back-fat layer of pigs both at 50 and 115 kg BW. Pig genotype affected the FA composition of both subcutaneous back-fat layers. The proportions of C18:0 and SFA in fat tissue were higher in IB than in LR × LW pigs from 38 to 65 kg BW, especially in the outer layer. In addition, MUFA contents were higher in IB pigs at 115 kg BW in both layers (+5% on average; P < 0.01). Increased proportions of C18:2 n-6 and polyunsaturated FA (PUFA) were found in LR × LW pigs, irrespective of the stage of growth and back-fat layer (P⩽0.02). At 50 kg BW, pigs receiving the high-protein diet presented the highest C18:2 n-6, C18:3 n-3, C20:5 n-3 and PUFA contents. A significant genotype × CP content interaction was observed for C18:3 n-3 because of the increased concentration of this FA in LR × LW pigs when offered the 17% CP diet (P < 0.05). Higher C16:0 and SFA contents (+5%; P = 0.03) were found in pigs offered the 13% CP diet and slaughtered at 115 kg BW. There was a genotype × CP interaction for MUFA concentration because of the higher MUFA content observed in IB pigs offered the highest protein content diet (P = 0.03). Our results suggest that genetic variation in de novo lipid synthesis and pattern of FA unsaturation might contribute to explain differences in back-fat FA profile of IB and LR × LW pigs under identical nutritional management. They could be also relevant to explain the low efficiency of nutrient and energy utilization in the IB pig.

Keywords

fatty acid profilesubcutaneous adipose tissuebody weightdietary proteinpig genotype
Type
Product quality, human health and well-being
Information
animal , Volume 7 , Issue 4 , April 2013 , pp. 688 - 698
Copyright
Copyright © The Animal Consortium 2012

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Footnotes

a

Supported by a contract from the JAE-postdoctoral Program (CSIC, Spain).

References

Antequera, T 1990. Evolución del componente lipídico durante la maduración del jamón de cerdo Ibérico. PhD, Extremadura University.Google Scholar
Barea, R, Nieto, R, Aguilera, JF 2007. Effects of the dietary protein content and the feeding level on protein and energy metabolism in IB pigs growing from 50 to 100 kg body weight. Animal 1, 357–365.CrossRefGoogle Scholar
Barea, R, Nieto, R, Vitari, F, Domeneghini, C, Aguilera, JF 2011. Effects of pig genotype (Iberian vs. Landrace × Large White) on nutrient digestibility, relative organ weights and small intestine structure at two stages of growth. Animal 5, 547–557.Google Scholar
Barea, R, Nieto, R, Lara, L, García, MA, Vílchez, MA, Aguilera, JF 2006. Effects of dietary protein content and feeding level on carcass characteristics and organ weights of Iberian pigs growing between 50 and 100 kg live weight. Animal Science 82, 405–413.Google Scholar
Bee, G, Gebert, S, Messikommer, R 2002. Effect of dietary energy supply and fat source on the fatty acid pattern of adipose and lean tissues and lipogenesis in the pig. Journal of Animal Science 80, 1564–1574.Google Scholar
Bligh, EG, Dyer, WJ 1959. A rapid method of total lipid extraction and purification. Canadian Journal of Animal Science 37, 911–917.Google Scholar
Boletín Oficial del Estado (BOE) 2004 . Orden PRE/3844/2004, de 18 de noviembre, por la que se establecen los métodos oficiales de toma de muestras en canales de cerdos ibéricos y el método de análisis para la determinación de la composición de ácidos grasos de los lípidos totales del tejido adiposo subcutáneo de cerdos ibéricos. Boletin Oficial del Estado 283, 38770-38779. Agencia Estatal Boletín Oficial del Estado, Madrid, Spain .Google Scholar
British Society of Animal Science (BSAS) 2003. Nutrient requirement standards for pigs. BSAS, Penicuik, UK.Google Scholar
Campbell, RG, Taverner, MR 1988. Genotype and sex effects on the relationship between energy intake and protein deposition in growing pigs. Journal of Animal Science 66, 676–686.Google Scholar
Conde-Aguilera, JA, Aguinaga, MA, Aguilera, JF, Nieto, R 2011. Nutrient and energy retention in weaned Iberian piglets fed diets with different protein concentrations. Journal of Animal Science 89, 754–763.Google Scholar
Daza, A, Mateos, A, Rey, AI, Ovejero, I, López-Bote, CJ 2007. Effect of duration of feeding under free-range conditions on production results and carcass and fat quality in Iberian pigs. Meat Science 76, 411–416.Google Scholar
Doran, O, Moule, SK, Teye, GA, Whittington, FM, Hallett, KG, Wood, JD 2006. A reduced protein diet induces stearoyl-CoA desaturase protein expression in pig muscle but not in subcutaneous adipose tissue: relationship with intramuscular lipid formation. British Journal of Nutrition 95, 609–617.Google Scholar
Freire, JPB, Mourot, J, Cunha, LF, Almeida, JAA, Aumaitre, A 1998. Effect of the source of dietary fat on postweaning lipogenesis in lean and fat pigs. Annals of Nutrition and Metabolism 42, 90–95.Google Scholar
Kloareg, M, Noblet, J, van Milgen, J 2007. Deposition of dietary fatty acids, de novo synthesis and anatomical partitioning of fatty acids in finishing pigs. British Journal of Nutrition 97, 35–44.Google Scholar
Lizardo, R, Le Bellego, L, Noblet, J, van Milgen, J, Mourot, J 2002a. Effet de la temperature d’élevage et de la nature du régime sur la composition en acides gras du tissu adipeux du porc. Journées de la Recherche Porcine 34, 45–51.Google Scholar
Lizardo, R, van Milgen, J, Mourot, J, Noblet, J, Bonneau, M 2002b. A nutritional model of fatty acid composition in the growing–finishing pig. Livestock Production Science 75, 167–182.Google Scholar
López-Bote, C, Rey, A, Ruiz, J, Isabel, B, Sanz Arias, R 1997. Effect of feeding diets high in monounsaturated fatty acids and α-tocopheryl acetate to rabbits on resulting carcass fatty acid profile and lipid oxidation. Animal Science 64, 177–186.Google Scholar
Martín, L, Timón, ML, Petrón, MJ, Ventanas, J, Antequera, T 2000. Evolution of volatile aldehydes in Iberian hams matured under different processing conditions. Meat Science 54, 333–337.Google Scholar
Morales, J, Pérez, JF, Baucells, MD, Mourot, J, Gasa, J 2002. Comparative digestibility and lipogenic activity in Landrace and Iberian finishing pigs fed ad libitum corn- and corn–sorghum–acorn-based diets. Livestock Production Science 77, 195–205.Google Scholar
Morales, J, Baucells, MD, Pérez, JF, Mourot, J, Gasa, J 2003. Body fat content, composition and distribution in Landrace and Iberian finishing pigs given ad libitum maize- and acorn–sorghum–maize-based diets. Animal Science 77, 215–224.Google Scholar
Mourot, J, Kouba, M, Bonneau, M 1996. Comparative study of in vitro lipogenesis in various adipose tissues in the growing Meishan pig: comparison with the Large White pig (Sus domesticus). Comparative Physiology and Biochemistry 115B, 383–388.Google Scholar
Nelson, DL, Cox, MM 2000. Lipid biosynthesis. In Lehninger principles of biochemistry (ed. Ryan M, Strange L, Neal V and Geller E), 3rd edition. Worth Publishers, New York, pp. 770–817.Google Scholar
Nieto, R, Miranda, A, García, MA, Aguilera, JF 2002. The effect of dietary protein content and feeding level on the rate of protein deposition and energy utilization in growing Iberian pigs from 15 to 50 kg body weight. British Journal of Nutrition 88, 39–49.Google Scholar
Nieto, R, Lara, L, García, MA, Vílchez, MA, Aguilera, JF 2003. Effects of dietary protein content and food intake on carcass characteristics and organ weights of growing Iberian pigs. Animal Science 77, 47–56.CrossRefGoogle Scholar
Nieto, R, Lara, L, García, MA, Gómez, F, Zalvide, M, Cruz, M, Pariente, JM, Moreno, A, Aguilera, JF 2001. Evaluation of an integrated feeding system in the Iberian pig. Study of food consumption and productive parameters. Sólo Cerdo Ibérico 6, 57–69.Google Scholar
Noblet, J, Karege, C, Dubois, S, van Milgen, J 1999. Metabolic utilization of energy and maintenance requirements in growing pigs: effects of sex and genotype. Journal of Animal Science 77, 1208–1216.Google Scholar
O'Hea, EK, Leveille, GA 1969. Significance of adipose tissue and liver as sites of fatty acid synthesis in the pig and the efficiency of utilization of various substrates for lipogenesis. Journal of Nutrition 99, 338–344.Google Scholar
Rivera-Ferre, MG, Aguilera, JF, Nieto, R 2006. Differences in whole-body protein turnover between Iberian and Landrace pigs fed adequate or lysine-deficient diets. Journal of Animal Science 84, 3346–3355.Google Scholar
Sauvant, D, Perez, JM, Tran, G 2004. Tables of composition and nutritional value of feed materials. Pigs, poultry, cattle, sheep, goats, rabbits, horses, fish. INRA Editions and AFZ, Paris, France.Google Scholar
Scott, RA, Cornelius, SG, Mersmann, HJ 1981. Fatty acid composition of adipose tissue from lean and obese swine. Journal of Animal Science 53, 977–981.Google Scholar
Serra, X, Gil, F, Pérez-Enciso, M, Oliver, MA, Vázquez, JM, Gispert, M, Díaz, I, Moreno, F, Latorre, R, Noguera, JL 1998. A comparison of carcass, meat quality and histochemical characteristics of Iberian (Guadyerbas line) and Landrace pigs. Livestock Production Science 56, 215–223.Google Scholar
Sukhija, PS, Palmquist, DL 1988. Rapid method for determination of total fatty acid content and composition of feedstuffs and feces. Journal of Agricultural and Food Chemistry 36, 1202–1206.Google Scholar
Teye, GA, Sheard, PR, Whittington, FM, Nute, GR, Stewart, A, Wood, JD 2006. Influence of dietary oils and protein level on pork quality. 1. Effects on muscle fatty acid composition, carcass, meat and eating quality. Meat Science 73, 157–165.Google Scholar
van Milgen, J, Bernier, JF, Lecozler, Y, Dubois, S, Noblet, J 1998. Major determinants of fasting heat production and energetic cost of activity in growing pigs of different body weight and breed/castration combination. British Journal of Nutrition 79, 509–517.Google Scholar
Wood, JD, Enser, M, Whittington, FM, Moncrieff, CB, Kempster, AJ 1989. Backfat composition in pigs: differences between fat thickness groups and sexes. Livestock Production Science 22, 351–362.Google Scholar
Wood, JD, Enser, M, Fisher, AV, Nute, GR, Sheard, PR, Richardson, RI, Hughes, SI, Whittington, FM 2008. Fat deposition, fatty acid composition and meat quality: a review. Meat Science 78, 343–358.CrossRefGoogle ScholarPubMed
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