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Evaluation of the VP22 gene adjuvant for enhancement of DNA vaccine against somatostatin in mice

Published online by Cambridge University Press:  01 November 2008

Li. Han ,
A. X. Liang ,
J. Zhang ,
M. Fang ,
G. H. Hua ,
L. Sang ,
L. Y. Geng ,
H. R. Wang  and
L. G. Yang
Li. Han
Affiliation:
Key lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 PRChina
A. X. Liang
Affiliation:
Key lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 PRChina
J. Zhang
Affiliation:
Key lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 PRChina
M. Fang
Affiliation:
Key lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 PRChina
G. H. Hua
Affiliation:
Key lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 PRChina
L. Sang
Affiliation:
Key lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 PRChina
L. Y. Geng
Affiliation:
Key lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 PRChina
H. R. Wang
Affiliation:
Key lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 PRChina
L. G. Yang*
Affiliation:
Key lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 PRChina
*
E-mail: [email protected]
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Abstract

Somatostatin (SS) is a hormone that inhibits the secretion of growth hormone. Immunization against SS can promote the growth of animals. A novel SS-VP22 fused vaccine, pEGS2SS-V, was constructed from pEGS2SS plasmid with a VP22 gene fragment. Two times of immunization with pEGS2SS-V-induced anti-SS antibodies in mice. Compared with mice immunized with pEGS2SS and 0.85% saline, the growth performance of mice immunized with pEGS2SS-V was increased by 14.1% (P < 0.05) and 48.4% (P < 0.01) on the 2nd week after the first vaccination, respectively. The results indicated that the effects of the somatostatin DNA vaccine could be improved effectively by VP22 gene adjuvant.

Keywords

BHV-1 VP22somatostatinDNA vaccine
Type
Full Paper
Information
animal , Volume 2 , Issue 11 , November 2008 , pp. 1569 - 1574
Copyright
Copyright © The Animal Consortium 2008

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