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Evaluation of swine fertilisation medium (SFM) efficiency in preserving spermatozoa quality during long-term storage in comparison to four commercial swine extenders

Published online by Cambridge University Press:  28 October 2008

P. Fantinati ,
A. Zannoni ,
C. Bernardini ,
M. Forni ,
A. Tattini ,
E. Seren  and
M. L. Bacci
P. Fantinati
Affiliation:
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia 40064 (BO), Italy
A. Zannoni
Affiliation:
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia 40064 (BO), Italy
C. Bernardini
Affiliation:
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia 40064 (BO), Italy
M. Forni
Affiliation:
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia 40064 (BO), Italy
A. Tattini
Affiliation:
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia 40064 (BO), Italy
E. Seren
Affiliation:
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia 40064 (BO), Italy
M. L. Bacci*
Affiliation:
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia 40064 (BO), Italy
*
E-mail: [email protected]
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Abstract

In pig production, artificial insemination is widely carried out and the use of fresh diluted semen is predominant. For this reason, there are increasing interests in developing new extenders and in establishing the optimal storage conditions for diluted spermatozoa. In the last few decades, we utilised a homemade diluent (swine fertilisation medium (SFM)) for spermatozoa manipulation and biotechnological application as the production of transgenic pigs utilising the sperm-mediated gene transfer technique. The purpose of the present study is therefore to analyse the ability of SFM, in comparison to four commercial extenders, in preserving the quality of diluted boar semen stored at 16.5°C till 15 days. We utilised some of the main predictive tests as objectively measured motility, acrosome and sperm membrane integrity, high mitochondrial membrane potential and pH. Based on our in vitro study, SFM could be declared as a good long-term extender, able to preserve spermatozoa quality as well as Androhep Enduraguard for up to 6 to 9 days and more.

Keywords

boarspermatozoa qualityextendersemen storage
Type
Full Paper
Information
animal , Volume 3 , Issue 2 , February 2009 , pp. 269 - 274
Copyright
Copyright © The Animal Consortium 2008

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