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Effect of pregnancy and feeding level on cell turnover and expression of related genes in the mammary tissue of lactating dairy cows

Published online by Cambridge University Press:  01 April 2008

J. V. Nørgaard ,
M. T. Sørensen ,
P. K. Theil ,
J. Sehested  and
K. Sejrsen
J. V. Nørgaard
Affiliation:
Department of Animal Health, Welfare and Nutrition, University of Aarhus, P.O. Box 50, DK-8830 Tjele, Denmark
M. T. Sørensen*
Affiliation:
Department of Animal Health, Welfare and Nutrition, University of Aarhus, P.O. Box 50, DK-8830 Tjele, Denmark
P. K. Theil
Affiliation:
Department of Animal Health, Welfare and Nutrition, University of Aarhus, P.O. Box 50, DK-8830 Tjele, Denmark
J. Sehested
Affiliation:
Department of Animal Health, Welfare and Nutrition, University of Aarhus, P.O. Box 50, DK-8830 Tjele, Denmark
K. Sejrsen
Affiliation:
Department of Animal Health, Welfare and Nutrition, University of Aarhus, P.O. Box 50, DK-8830 Tjele, Denmark
*
E-mail: [email protected]
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Abstract

Milk yield is reduced by pregnancy, and the present experiment was conducted to study the biological basis for the negative effect of pregnancy on milk yield. A total of 16 dairy cows were fed at either a normal or a low feeding level (eight cows per treatment), and half of them were inseminated after approximately 3 months of lactation and the other half were not inseminated. Mammary biopsies were taken at approximately 9 months of lactation. The milk yield of pregnant cows was reduced by 2.6 kg/day, and lactation persistency was reduced already from the time of insemination. Low feeding level reduced the milk yield by 9.8 kg/day from week 8 to week 39 of lactation, whereas no interaction between pregnancy and feeding level was found. Cell proliferation (Ki-67) and apoptosis (terminal deoxynucleotidyl transferase dUTP nick end labeling, TUNEL) were unaffected by feeding level, and pregnancy tended to reduce cell proliferation but had no effect on apoptosis. Reduced cell proliferation may explain the reduced lactation persistency in pregnant cows. Transcription of oestrogen receptor α, progesterone receptor A and B, and long and short isoforms of the prolactin receptor were higher in pregnant cows compared with non-pregnant cows. Feeding level did not mediate changes in transcription of genes. Transcription of other cell-turnover-related genes (IGF-I, IGF binding protein-5, caspase-3) as well as genes related to the secretory activity of the cells (α-lactalbumin and acetyl CoA carboxylase α) was not affected by pregnancy or by feeding level.

Keywords

mammary cell turnoverpregnancyfeeding levelgene expressiondiary cows
Type
Full Paper
Information
animal , Volume 2 , Issue 4 , April 2008 , pp. 588 - 594
Copyright
Copyright © The Animal Consortium 2008

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References

Akers, RM 2002. Lactation and the mammary gland. Iowa State Press, Ames.Google Scholar
Anderson, E, Clarke, RB 2004. Steroid receptors and cell cycle in normal mammary epithelium. Journal of Mammary Gland Biology and Neoplasia 9, 3–13.CrossRefGoogle ScholarPubMed
Auchtung, TL, Rius, AG, Kendall, PE, McFadden, TB, Dahl, GE 2005. Effects of photoperiod during the dry period on prolactin, prolactin receptor, and milk production of dairy cows. Journal of Dairy Science 88, 121–127.CrossRefGoogle ScholarPubMed
Bertilsson, J, Berglund, B, Ratnayake, G, SvennerstenSjaunja, K, Wiktorsson, H 1997. Optimising lactation cycles for the high-yielding dairy cow. A European perspective. Livestock Production Science 50, 5–13.CrossRefGoogle Scholar
Byatt, JC, Eppard, PJ, Veenhuizen, JJ, Curran, TL, Curran, DF, McGrath, MF, Collier, RJ 1994. Stimulation of mammogenesis and lactogenesis by recombinant bovine placental lactogen in steroid-primed dairy heifers. Journal of Endocrinology 140, 33–43.CrossRefGoogle ScholarPubMed
Cassy, S, Charlier, M, Bélair, L, Guillomot, M, Charron, G, Bloch, B, Djiane, J 1998. Developmental expression and localization of the prolactin receptor (PRL-R) gene in ewe mammary gland during pregnancy and lactation: estimation of the ratio of the two forms of PRL-R messenger ribonucleic acid. Biology of Reproduction 58, 1290–1296.CrossRefGoogle ScholarPubMed
Clarke, R 2000. Introduction and overview: sex steroids in the mammary gland. Journal of Mammary Gland Biology and Neoplasia 5, 245–250.CrossRefGoogle ScholarPubMed
Connor, EE, Wood, DL, Sonstegard, TS, da Mota, AF, Bennett, GL, Williams, JL, Capuco, AV 2005. Chromosomal mapping and quantitative analysis of estrogen-related receptor alpha-1, estrogen receptors alpha and beta and progesterone receptor in the bovine mammary gland. Journal of Endocrinology 185, 593–603.CrossRefGoogle ScholarPubMed
Connor, EE, Meyer, MJ, Li, RW, van Amburgh, ME, Boisclair, YR, Capuco, AV 2007. Regulation of gene expression in the bovine mammary gland by ovarian steroids. Journal of Dairy Science 90, E55–E65.CrossRefGoogle ScholarPubMed
Hanstein, B, Djahansouzi, S, Dall, P, Beckmann, MW, Bender, HG 2004. Insights into the molecular biology of the estrogen receptor define novel therapeutic targets for breast cancer. European Journal of Endocrinology 150, 243–255.CrossRefGoogle ScholarPubMed
Knight, CH, Wilde, CJ 1993. Mammary cell changes during pregnancy and lactation. Livestock Production Science 35, 3–19.CrossRefGoogle Scholar
Lacasse, P, Block, E, Petitclerc, D 1994. Effect of plane of nutrition before and during gestation on the concentration of hormones in dairy Heifers. Journal of Dairy Science 77, 439–445.CrossRefGoogle ScholarPubMed
Lamote, I, Meyer, E, Massart-Leen, AM, Burvenich, C 2004. Sex steroids and growth factors in the regulation of mammary gland proliferation, differentiation, and involution. Steroids 69, 145–159.CrossRefGoogle ScholarPubMed
Mulac-Jericevic, B, Conneely, OM 2004. Reproductive tissue selective actions of progesterone receptors. Reproduction 128, 139–146.CrossRefGoogle ScholarPubMed
Nørgaard, J, Sorensen, A, Sørensen, MT, Andersen, JB, Sejrsen, K 2005. Mammary cell turnover and enzyme activity in dairy cows: effects of milking frequency and diet energy density. Journal of Dairy Science 88, 975–982.CrossRefGoogle ScholarPubMed
Olazabal, I, Munoz, J, Ogueta, S, Obregon, E, Garcia-Ruiz, JP 2000. Prolactin (PRL)-PRL receptor system increases cell proliferation involving JNK (c-Jun amino terminal kinase) and AP-1 activation: inhibition by glucocorticoids. Molecular Endocrinology 14, 564–575.CrossRefGoogle ScholarPubMed
Palmieri, C, Cheng, GJ, Saji, S, Zelada-Hedman, M, Warri, A, Weihua, Z, Van Noorden, S, Wahlstrom, T, Coombes, RC, Warner, M, Gustafsson, JA 2002. Estrogen receptor beta in breast cancer. Endocrine-Related Cancer 9, 1–13.CrossRefGoogle ScholarPubMed
Pfaffl, MW 2001. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research 29, article no. e45.CrossRefGoogle ScholarPubMed
Pfaffl, MW, Lange, IG, Daxenberger, A, Meyer, HHD 2001. Tissue-specific expression pattern of estrogen receptors (ER): quantification of ER alpha and ER beta mRNA with real-time RT-PCR. Acta Pathologica, Microbiologica, et Immunologica Scandinavica (APMIS) 109, 345–355.CrossRefGoogle ScholarPubMed
Rasmussen, RP 2001. Quantification on the LightCycler. In Rapid cycle real-time PCR, methods and applications (ed. S Meuer, CT Wittwer and K Nakagawara), pp. 21–34. Springer Press, Heidelberg.CrossRefGoogle Scholar
SAS Institute 1999. Release 8.2. SAS Institute Inc., Cary, NC.Google Scholar
Schams, D, Russe, I, Schallenberger, E, Prokopp, S, Chan, JS 1984. The role of steroid hormones, prolactin and placental lactogen on mammary gland development in ewes and heifers. Journal of Endocrinology 102, 121–130.CrossRefGoogle ScholarPubMed
Schams, D, Kohlenberg, S, Amselgruber, W, Berisha, B, Pfaffl, MW, Sinowatz, F 2003. Expression and localisation of oestrogen and progesterone receptors in the bovine mammary gland during development, function and involution. Journal of Endocrinology 177, 305–317.CrossRefGoogle ScholarPubMed
Schuler, LA, Nagel, RJ, Gao, J, Horseman, ND, Kessler, MA 1997. Prolactin receptor heterogeneity in bovine fetal and maternal tissues. Endocrinology 138, 3187–3194.CrossRefGoogle ScholarPubMed
Sehested, J, Danfær, A 2003. Prolonged calving interval and reduced supplementation in an organic dairy herd. In Book of abstracts of the 54th annual meeting of the European Association for Animal Production (ed. A Hofer, G Zervas, E von Borell, C Knight, C Lazzaroni, M Sneeberger, C Wenk and W Martin-Rosset), p. 279. Wageningen Academic Publishers, Wageningen, The Netherlands.Google Scholar
Smith, JJ, Capuco, AV, Akers, RM 1987. Quantification of progesterone binding in mammary tissue of pregnant ewes. Journal of Dairy Science 70, 1178–1185.CrossRefGoogle ScholarPubMed
Sorensen, A, Knight, CH 2002. Endocrine profiles of cows undergoing extended lactation in relation to the control of lactation persistency. Domestic Animal Endocrinology 23, 111–123.CrossRefGoogle Scholar
Sørensen, MT, Nørgaard, JV, Theil, PK, Vestergaard, M, Sejrsen, K 2006. Cell turnover and activity in mammary tissue during lactation and the dry period in dairy cows. Journal of Dairy Science 89, 4632–4639.CrossRefGoogle ScholarPubMed
Takahashi, T 2006. Biology of the prolactin family in bovine placenta. I. Bovine placental lactogen: expression, structure and proposed roles. Animal Science Journal 77, 10–17.CrossRefGoogle Scholar
Theil, PK, Sorensen, IL, Therkildsen, M, Oksbjerg, N 2006. Changes in proteolytic enzyme mRNAs relevant for meat quality during myogenesis of primary porcine satellite cells. Meat Science 73, 335–343.CrossRefGoogle ScholarPubMed
van Amburgh, ME, Galton, DM, Bauman, DE, Everett, RW 1997. Management and economics of extended calving intervals with use of bovine somatotropin. Livestock Production Science 50, 15–28.CrossRefGoogle Scholar
Warner, M, Saji, S, Gustafsson, JA 2000. The normal and malignant mammary gland: a fresh look with ER beta onboard. Journal of Mammary Gland Biology and Neoplasia 5, 289–294.CrossRefGoogle ScholarPubMed