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Effect of ferrous sulphate and ascorbic acid on motility, viability and lipid peroxidation of crossbred cattle bull spermatozoa

Published online by Cambridge University Press:  01 January 2008

A. K. Bansal  and
G. S. Bilaspuri
A. K. Bansal*
Affiliation:
Department of Zoology, Punjab Agricultural University, Ludhiana 141004, India
G. S. Bilaspuri
Affiliation:
Department of Zoology, Punjab Agricultural University, Ludhiana 141004, India
*
E-mail: [email protected]
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Abstract

Numerous factors influence male fertility, one of these being the oxidative stress, which has elicited enormous interest recently. In sperm, induction of oxidation decreases motility and viability but increases lipid peroxidation (LPO). The optimum dose of ferrous ascorbate (FeAA: FeSO4 + ascorbic acid) for inducing oxidative stress by affecting motility, viability and LPO has been ascertained in local crossbred cattle bull spermatozoa. The fractions of spermatozoa suspended in 2.9% sodium citrate were subjected to three doses of FeAA (100 : 500, 150 : 750, 200 : 1000; μmol/l FeSO4 : μmol/l ascorbic acid). These fractions were assessed for various parameters. Increase in the incubation period and promoter concentration induced a decrease in motility and viability, but an increase in LPO. Among three doses of FeAA, 150 : 750 μmol/l ascorbic acid is suggested to be the optimum/best dose as it induces the oxidative stress/LPO to a significant extent and also maintains better motility and viability as compared with the other two doses, and such conditions may enhance the fertilising potential of bull spermatozoa.

Keywords

ferrous ascorbatelipid peroxidationreactive oxygen speciessperm motilityviability
Type
Full Paper
Information
animal , Volume 2 , Issue 1 , January 2008 , pp. 100 - 104
Copyright
Copyright © The Animal Consortium 2008

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References

Agarwal, A, Prabakaran, SA 2005. Mechanism, measurement and prevention of oxidative stress in male reproductive physiology. Indian Journal of Experimental Biology 43, 963–974.Google ScholarPubMed
Aitken, RJ, Clarkson, JS, Fishel, S 1989. Generation of reactive oxygen species, lipid peroxidation and human sperm function. Biology of Reproduction 41, 183–197.CrossRefGoogle ScholarPubMed
Aitken, RJ, Harkiss, D, Buckingham, D 1993. Relationship between iron-catalysed lipid peroxidation potential and human sperm function. Journal of Reproduction and Fertility 98, 257–265.Google Scholar
Blom, E 1977. The evaluation of bull semen with special reference to its use in artificial insemination. Animal Breeding Abstracts 19, 648.Google Scholar
Buege, JA, Aust, SD 1978. Microsomal lipid peroxidation. Methods in Enzymology 52, 302–310.CrossRefGoogle ScholarPubMed
De Lamirande, E, Jiang, H, Zini, A, Kodama, H, Gagnon, C 1997. Reactive oxygen species and sperm physiology. Reviews of Reproduction 2, 48–54.Google Scholar
Jones, R, Mann, T 1977. Damage to ram spermatozoa by peroxidation of endogenous phospholipids. Journal of Reproduction and Fertility 50, 261–268.Google Scholar
Jones, R, Mann, T, Sherin, R 1979. Peroxidative breakdown of phospholipids in human spermatozoa, spermicidal properties of fatty acids peroxides and prospective action of seminal plasma. Fertility and Sterility 31, 531–537.CrossRefGoogle Scholar
Kalla, NR, Vasudev, M 1981. Effect of gossypol acetic acid in the motility and ATPase activity of human spermatozoa. Andrologia 13, 95–98.CrossRefGoogle ScholarPubMed
Kodama, H, Kribayashi, Y, Gagnon, C 1996. Effect of sperm lipid peroxidation on fertilization. Journal of Andrology 17, 151–157.Google Scholar
Lees, MB, Paxman, S 1972. Modification Lowry procedure for the analysis of proteolipid protein. Analytical Biochemistry 47, 184–192.CrossRefGoogle ScholarPubMed
Mann, T, Mann, CL 1981. Male reproductive function and semen. Springer-Verlag, New York, USA.Google Scholar
Rao, B, Soufir, JC, Martin, M, David, G 1989. Lipid peroxidation in human spermatozoa as related to mid piece abnormalities and motility. Gamete Research 24, 127–134.CrossRefGoogle Scholar
Sharma, RK, Agarwal, A 1996. Role of reactive oxygen species in male infertility. Urology 48, 835–850.CrossRefGoogle ScholarPubMed
Singh, P, Chand, D, Georgie, GC 1989. Effect of vitamin E on lipid peroxidation in buffalo Bubalis bubalus L. Indian Journal of Experimental Biology 27, 14–16.Google Scholar
Storey, BT 1997. Biochemistry of the induction and prevention of lipoperoxidative damage in human spermatozoa. Molecular Human Reproduction 3, 203–213.Google Scholar
Verma, A, Kanwar, KC 1999. Effect of vitamin E on human sperm motility and lipid peroxidation in vitro. Asian Journal of Andrology 1, 151–154.Google ScholarPubMed