Hostname: page-component-586b7cd67f-r5fsc Total loading time: 0 Render date: 2024-11-27T23:59:34.510Z Has data issue: false hasContentIssue false
  • English
  • Français

Differential mesenteric fat deposition in bovines fed on silage or concentrate is independent of glycerol membrane permeability

Published online by Cambridge University Press:  15 July 2011

A. P. Martins ,
P. A. Lopes ,
A. S. H. Costa ,
S. V. Martins ,
N. C. Santos ,
J. A. M. Prates ,
T. F. Moura  and
G. Soveral
A. P. Martins
Affiliation:
REQUIMTE, Dep. Química, FCT-UNL, 2829-516 Caparica, Portugal
P. A. Lopes
Affiliation:
CIISA, Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, 1300-477 Lisboa, Portugal
A. S. H. Costa
Affiliation:
CIISA, Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, 1300-477 Lisboa, Portugal
S. V. Martins
Affiliation:
CIISA, Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, 1300-477 Lisboa, Portugal
N. C. Santos
Affiliation:
Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal
J. A. M. Prates
Affiliation:
CIISA, Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, 1300-477 Lisboa, Portugal
T. F. Moura
Affiliation:
REQUIMTE, Dep. Química, FCT-UNL, 2829-516 Caparica, Portugal
G. Soveral*
Affiliation:
REQUIMTE, Dep. Química, FCT-UNL, 2829-516 Caparica, Portugal Faculdade de Farmácia, Universidade de Lisboa, 1649-003 Lisboa, Portugal
*
†E-mail: [email protected]; [email protected]
Get access

Abstract

In the meat industry, the manipulation of fat deposition in cattle is of pivotal importance to improve production efficiency, carcass composition and ultimately meat quality. There is an increasing interest in the identification of key factors and molecular mechanisms responsible for the development of specific fat depots. This study aimed at elucidating the influence of breed and diet on adipose tissue membrane permeability and fluidity and their interplay on fat deposition in bovines. Two Portuguese autochthonous breeds, Alentejana and Barrosã, recognized as late- and early-maturing breeds, respectively, were chosen to examine the effects of breed and diet on fat deposition and on adipose membrane composition and permeability. Twenty-four male bovines from these breeds were fed on silage-based or concentrate-based diets for 11 months. Animals were slaughtered to determine their live slaughter and hot carcass weights, as well as weights of subcutaneous and visceral adipose depots. Mesenteric fat depots were excised and used to isolate adipocyte membrane vesicles where cholesterol content, fatty acid profile as well as permeability and fluidity were determined. Total accumulation of neither subcutaneous nor visceral fat was influenced by breed. In contrast, mesenteric and omental fat depots weights were higher in concentrate-fed bulls relative to silage-fed animals. Membrane fluidity and permeability to water and glycerol in mesenteric adipose tissue were found to be independent of breed and diet. Moreover, the deposition of cholesterol and unsaturated fatty acids, which may influence membrane properties, were unchanged among experimental groups. Adipose membrane lipids from the mesenteric fat depot of ruminants were rich in saturated fatty acids, and unaffected by polyunsaturated fatty acids dietary levels. Our results provide evidence against the involvement of cellular membrane permeability to glycerol on fat accumulation in mesenteric fat tissue of concentrate-fed bovines, which is consistent with the unchanged membrane lipid profile found among experimental groups.

Keywords

adipose membraneglycerol permeabilitymembrane fluiditylipid compositionbovine breeds
Type
Full Paper
Information
animal , Volume 5 , Issue 12 , 10 November 2011 , pp. 1949 - 1956
Copyright
Copyright © The Animal Consortium 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

a

These authors have contributed equally to this article.

References

Alfaia, CMM, Castro, MLF, Martins, SIV, Portugal, APV, Alves, SPA, Fontes, CMGA, Bessa, RJB, Prates, JAM 2007. Effect of slaughter season on fatty acid composition, conjugated linoleic acid isomers and nutritional value of intramuscular fat in Barrosã-PDO veal. Meat Science 75, 44–52.CrossRefGoogle ScholarPubMed
Alfaia, CPM, Alves, SP, Martins, SIV, Costa, ASH, Fontes, CMGA, Lemos, JPC, Bessa, RJB, Prates, JAM 2009. Effect of the feeding system on intramuscular fatty acids and conjugated linoleic acid isomers of beef cattle, with emphasis on their nutritional value and discriminatory ability. Food Chemistry 114, 939–946.CrossRefGoogle Scholar
Alves, SP, Bessa, RJ 2009. Comparison of two gas–liquid chromatograph columns for the analysis of fatty acids in ruminant meat. Journal of Chromatography A 1216, 5130–5139.CrossRefGoogle ScholarPubMed
Azain, MJ 2004. Role of fatty acids in adipocyte growth and development. Journal of Animal Science 82, 916–924.CrossRefGoogle ScholarPubMed
Beja-Pereira, A, Alexandrino, P, Bessa, I, Carretero, Y, Dunner, S, Ferrand, N, Jordana, J, Laloe, D, Moazami-Goudarzi, K, Sanchez, A, Canon, J 2003. Genetic characterization of southwestern European bovine breeds: a historical and biogeographical reassessment with a set of 16 microsatellites. Journal of Heredity 94, 243–250.CrossRefGoogle Scholar
Bhattacharya, A, Banu, J, Rahman, M, Causey, J, Fernandes, G 2006. Biological effects of conjugated linoleic acids in health and disease. Journal of Nutritional Biochemistry 17, 789–810.CrossRefGoogle ScholarPubMed
Bradford, MM 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Analytical Biochemistry 72, 248–254.CrossRefGoogle ScholarPubMed
Christie, WW, Dobson, G, Adlof, RO 2007. A practical guide to the isolation, analysis and identification of conjugated linoleic acid. Lipids 42, 1073–1084.CrossRefGoogle Scholar
Clandinin, MT, Field, CJ, Hargreaves, K, Morson, L, Zsigmond, E 1985. Role of diet fat in subcellular structure and function. Canadian Journal of Physiology and Pharmacology 63, 546–556.CrossRefGoogle ScholarPubMed
da Silva, MF, Lemos, JPC, Monteiro, LS, Portugal, AV 1998. Studies on growth and form: multivariate analysis of distribution of muscle and fat in Portuguese cattle breeds. Livestock Production Science 55, 261–271.CrossRefGoogle Scholar
Daniel, ZCTR, Wynn, RJ, Salter, AM, Buttery, PJ 2004. Differing effects of forage and concentrate diets on the oleic acid and conjugated linoleic acid content of sheep tissues: the role of stearoyl-CoA desaturase. Journal of Animal Science 82, 747–758.CrossRefGoogle ScholarPubMed
De Smet, S, Raes, K, Demeyer, D 2004. Meat fatty acid composition as affected by fatness and genetic factors: a review. Animal Research 53, 81–98.CrossRefGoogle Scholar
Dix, JA, Ausiello, DA, Jung, CY, Verkman, AS 1985. Target analysis studies of red cell water and urea transport. Biochimica et Biophysica Acta 821, 243–252.CrossRefGoogle ScholarPubMed
Enser, M, Hallett, K, Hewitt, B, Fursey, GAJ, Wood, JD 1996. Fatty acid content and composition of English beef, lamb and pork at retail. Meat Science 42, 443–456.CrossRefGoogle Scholar
Field, CJ, Clandinin, MT 1984. Modulation of adipose-tissue fat composition by diet – a review. Nutrition Research 4, 743–755.CrossRefGoogle Scholar
Field, CJ, Ryan, EA, Thomson, ABR, Clandinin, MT 1988. Dietary-fat and the diabetic state alter insulin binding and the fatty acyl composition of the adipocyte plasma-membrane. Biochemical Journal 253, 417–424.CrossRefGoogle ScholarPubMed
Hocquette, JF, Gondret, F, Baeza, E, Medale, F, Jurie, C, Pethick, DW 2010. Intramuscular fat content in meat-producing animals: development, genetic and nutritional control, and identification of putative markers. Animal 4, 303–319.CrossRefGoogle ScholarPubMed
Jambrenghi, AC, Paglialonga, G, Gnoni, A, Zanotti, F, Giannico, F, Vonghia, G, Gnoni, GV 2007. Changes in lipid composition and lipogenic enzyme activities in liver of lambs fed omega-6 polyunsaturated fatty acids. Comparative Biochemistry and Physiology B – Biochemistry & Molecular Biology 147, 498–503.CrossRefGoogle Scholar
Jenkins, TC 1994. Regulation of lipid-metabolism in the rumen. The Journal of Nutrition 124, S1372–S1376.CrossRefGoogle ScholarPubMed
Keating, AF, Kennelly, JJ, Zhao, FQ 2006. Characterization and regulation of the bovine stearoyl-CoA desaturase gene promoter. Biochemical and Biophysical Research Communications 344, 233–240.CrossRefGoogle ScholarPubMed
Lakowicz, JR 1999. Principles of fluorescence spectroscopy. Kluwer Academic/Plenum, New York.CrossRefGoogle Scholar
Lande, MB, Donovan, JM, Zeidel, ML 1995. The relationship between membrane fluidity and permeabilities to water, solutes, ammonia, and protons. Journal of General Physiology 106, 67–84.CrossRefGoogle ScholarPubMed
Lock, AL, Corl, BA, Barbano, DM, Bauman, DE, Ip, C 2004. The anticarcinogenic effect of trans-11 18:1 is dependent on its conversion to cis-9, trans-11 CLA by delta 9-desaturase in rats. The Journal of Nutrition 134, 2698–2704.CrossRefGoogle Scholar
Martins, AP, Lopes, PA, Martins, SV, Madeira, A, Santos, NC, Prates, JA, Moura, TF, Soveral, G 2010. Conjugated linoleic acid reduces permeability and fluidity of adipose plasma membranes from obese Zucker rats. Biochemical and Biophysical Research Communications 398, 199–204.CrossRefGoogle ScholarPubMed
Mukesh, M, Bionaz, M, Graugnard, DE, Drackley, JK, Loor, JJ 2010. Adipose tissue depots of Holstein cows are immune responsive: inflammatory gene expression in vitro. Domestic Animal Endocrinology 38, 168–178.CrossRefGoogle ScholarPubMed
Naeemi, ED, Ahmad, N, al-Sharrah, TK, Behbahani, M 1995. Rapid and simple method for determination of cholesterol in processed food. Journal of AOAC International 78, 1522–1525.CrossRefGoogle ScholarPubMed
Novakofski, J 2004. Adipogenesis: usefulness of in vitro and in vivo experimental models. Journal of Animal Science 82, 905–915.CrossRefGoogle ScholarPubMed
Onuki, Y, Hagiwara, C, Sugibayashi, K, Takayama, K 2008. Specific effect of polyunsaturated fatty acids on the cholesterol-poor membrane domain in a model membrane. Chemical & Pharmaceutical Bulletin 56, 1103–1109.CrossRefGoogle Scholar
Pond, CM 1999. Physiological specialisation of adipose tissue. Progress in Lipid Research 38, 225–248.CrossRefGoogle ScholarPubMed
Raes, K, De Smet, S, Demeyer, D 2001. Effect of double-muscling in Belgian Blue young bulls on the intramuscular fatty acid composition with emphasis on conjugated linoleic acid and polyunsaturated fatty acids. Animal Science 73, 253–260.CrossRefGoogle Scholar
Reis, C, Navas, D, Pereira, M, Cravador, A 2001. Growth hormone ALUI polymorphism analysis in eight Portuguese bovine breeds. Archivos de Zootecnia 50, 41–48.Google Scholar
Sarkkinen, ES, Agren, JJ, Ahola, I, Ovaskainen, ML, Uusitupa, MIJ 1994. Fatty-acid composition of serum-cholesterol esters, and erythrocyte and platelet membranes as indicators of long-term adherence to fat-modified diets. American Journal of Clinical Nutrition 59, 364–370.CrossRefGoogle ScholarPubMed
Simões, JA, Mendes, I 2003. Distribution of tissues in carcasses at the same proportion of total fat in Portuguese cattle breeds. Animal Research 52, 287–298.CrossRefGoogle Scholar
Soveral, G, Martins, AP, Martins, SV, Lopes, PA, Alfaia, CM, Prates, JA, Moura, TF 2009. Effect of dietary conjugated linoleic acid isomers on water and glycerol permeability of kidney membranes. Biochemical and Biophysical Research Communications 383, 108–112.CrossRefGoogle ScholarPubMed
Stillwell, W, Wassall, SR 2003. Docosahexaenoic acid: membrane properties of a unique fatty acid. Chemistry and Physics of Lipids 126, 1–27.CrossRefGoogle ScholarPubMed
Stubbs, CD, Smith, AD 1984. The modification of mammalian membrane poly-unsaturated fatty-acid composition in relation to membrane fluidity and function. Biochimica et Biophysica Acta 779, 89–137.CrossRefGoogle Scholar
van Heeswijk, MP, van Os, CH 1986. Osmotic water permeabilities of brush border and basolateral membrane vesicles from rat renal cortex and small intestine. Journal of Membrane Biology 92, 183–193.CrossRefGoogle ScholarPubMed
Vernon, RG, Houseknecht, KL 1991. Adipose tissue: beyond an energy reserve. In Ruminant physiology: digestion, metabolism, growth and reproduction (ed. PB Cronjé), pp. 171–186. CAB International, London.Google Scholar
Wachira, AM, Sinclair, LA, Wilkinson, RG, Enser, M, Wood, JD, Fisher, AV 2002. Effects of dietary fat source and breed on the carcass composition, n-3 polyunsaturated fatty acid and conjugated linoleic acid content of sheep meat and adipose tissue. British Journal of Nutrition 88, 697–709.CrossRefGoogle ScholarPubMed