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Are sainfoin or protein supplements alternatives to control small strongyle infection in horses?

Published online by Cambridge University Press:  24 May 2017

C. Collas ,
G. Sallé ,
B. Dumont ,
J. Cabaret ,
J. Cortet ,
W. Martin-Rosset ,
L. Wimel  and
G. Fleurance
C. Collas*
Affiliation:
Direction des Connaissances et de l’Innovation, IFCE, 49400 Saumur, France UMR1213 Herbivores, INRA et Vetagro Sup, 63122 Saint-Genès-Champanelle, France
G. Sallé
Affiliation:
UMR1282 Infectiologie et Santé Publique, INRA et Université François Rabelais Tours, 37380 Nouzilly, France
B. Dumont
Affiliation:
UMR1213 Herbivores, INRA et Vetagro Sup, 63122 Saint-Genès-Champanelle, France
J. Cabaret
Affiliation:
UMR1282 Infectiologie et Santé Publique, INRA et Université François Rabelais Tours, 37380 Nouzilly, France
J. Cortet
Affiliation:
UMR1282 Infectiologie et Santé Publique, INRA et Université François Rabelais Tours, 37380 Nouzilly, France
W. Martin-Rosset
Affiliation:
UMR1213 Herbivores, INRA et Vetagro Sup, 63122 Saint-Genès-Champanelle, France
L. Wimel
Affiliation:
Direction des Connaissances et de l’Innovation, IFCE, 49400 Saumur, France
G. Fleurance
Affiliation:
Direction des Connaissances et de l’Innovation, IFCE, 49400 Saumur, France UMR1213 Herbivores, INRA et Vetagro Sup, 63122 Saint-Genès-Champanelle, France
*
†E-mail: [email protected]
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Abstract

The spread of anthelmintic resistance in equine strongyle nematodes has become a major problem, advocating for the development of alternative control for strongyles. Our study consisted of both in vivo and in vitro experiments. We investigate for the first time the efficacy of a short-term consumption of tannin-rich sainfoin (Onobrychis viciifolia) or extra proteins in naturally infected horses. We used 30 horses allocated into three groups of 10 individuals that received for 18 days either (i) a tannin-rich diet with 70% DM sainfoin pellets (Sd), (ii) a protein-rich diet with 52% DM Italian rye-grass pellets and 18% DM grinded linseed expeller (Pd), or (iii) a control diet with 45% DM barley and 25% DM cereal-based pellets (Cd). The three diets were isoenergetic, covering 94% of animal energy requirements on average, and the Sd and Pd diets were isoproteic and provided extra proteins (227% of protein requirements v. 93% for the Cd diet). Pd and Cd were compared to test for benefits of receiving extra proteins, while Sd and Pd were compared to account for the effect of sainfoin secondary metabolites. There were no between-diet differences in faecal egg counts (FEC) or in worm burden evaluated from worm counts in faeces of drenched horses at the end of the experiment. However, coprocultures from the faeces collected in each group at the beginning and at the end of the experiment suggested a lower rate of strongyle larval development in the Sd group at the end of the experiment (Sd=8.1%, Pd=30.5%, Cd=22.6%). In vitro tests using sainfoin solutions evidenced the influence of sainfoin on strongyle larval development: adding 29% of sainfoin pellets to faeces reduced the strongyle egg development into infective larvae by 82% (P<0.001) and using solutions with sainfoin concentrations higher than 7.5 mg/ml reduced egg hatching by 37% (P<0.05). The short-term use of tannin-rich plants in horse diet could thus constitute a promising strategy to reduce the risk of infection by strongyles at pasture.

Keywords

equidtanninnitrogennematodenutrition
Type
Research Article
Information
animal , Volume 12 , Issue 2 , February 2018 , pp. 359 - 365
Copyright
© The Animal Consortium 2017 

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Footnotes

a

Present address: UR Animal et Fonctionnalités des Produits Animaux (USC340), INRA-Université de Lorraine, 54505 Vandœuvre-lès-Nancy Cedex, France.

References

Brunet, S, Jackson, F and Hoste, H 2008. Effects of sainfoin (Onobrychis viciifolia) extract and monomers of condensed tannins on the association of abomasal nematode larvae with fundic explants. International Journal for Parasitology 38, 783–790.CrossRefGoogle ScholarPubMed
Coles, GC, Bauer, C, Borgsteede, FHM, Geerts, S, Klei, TR, Taylor, MA and Waller, PJ 1992. World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Veterinary Parasitology 44, 35–44.CrossRefGoogle ScholarPubMed
Collas, C, Fleurance, G, Cabaret, J, Martin-Rosset, W, Wimel, L, Cortet, J and Dumont, B 2014. How does the suppression of energy supplementation affect herbage intake, performance and parasitism in lactating saddle mares? Animal 8, 1290–1297.Google Scholar
Coop, RL and Kyriazakis, I 1999. Nutrition-parasite interaction. Veterinary Parasitology 84, 187–204.Google Scholar
Couto, M, Santos, AS, Laborda, J, Nóvoa, M, Ferreira, LM and Madeira de Carvalho, LM 2016. Grazing behaviour of Miranda donkeys in a natural mountain pasture and parasitic level changes. Livestock Science 186, 16–21.Google Scholar
Desrues, O, Peña-Espinoza, M, Hansen, TVA, Enemark, HL and Thamsborg, SM 2016. Anti-parasitic activity of pelleted sainfoin (Onobrychis viciifolia) against Ostertagia ostertagi and Cooperia oncophora in calves. Parasites and Vectors 9, 329.Google Scholar
Donaldson, J, van Houtert, MFJ and Sykes, AR 2001. The effect of dietary fish-meal supplementation on parasite burdens of periparturient sheep. Animal Science 72, 149–158.Google Scholar
Etter, E, Hoste, H, Chartier, C, Pors, I, Koch, C, Broqua, C and Coutineau, H 2000. The effect of two levels of dietary protein on resistance and resilience of dairy goats experimentally infected with Trichostrongylus colubriformis: comparison between high and low producers. Veterinary Research 31, 247–258.Google Scholar
Feboli, A, Laurentiz, AC, Soares, SCS, Augusto, JG, Anjos, LA, Magalhães, LG, Filardi, RS and Laurentiz, RS 2016. Ovicidal and larvicidal activity of extracts of Opuntia ficus-indica against gastrointestinal nematodes of naturally infected sheep. Veterinary Parasitology 226, 65–68.Google Scholar
Grabber, JH, Zeller, WE and Mueller-Harvey, I 2013. Acetone enhances the direct analysis of procyanidin- and prodelphinidin-based condensed tannins in lotus species by the butanol-HCl-iron assay. Journal of Agricultural and Food Chemistry 61, 2669–2678.Google Scholar
Heckendorn, F, Häring, DA, Maurer, V, Zinsstag, J, Langhans, W and Hertzberg, H 2006. Effect of sainfoin (Onobrychis viciifolia) silage and hay on established populations of Haemonchus contortus and Cooperia curticei in lambs. Veterinary Parasitology 142, 293–300.CrossRefGoogle ScholarPubMed
Hoste, H, Jackson, F, Athanasiadou, S, Thamsborg, SM and Hoskin, SO 2006. The effects of tannin-rich plants on parasitic nematodes in ruminants. Trends in Parasitology 22, 253–261.Google Scholar
Houdijk, JGM, Kyriazakis, I, Jackson, F, Huntley, JF and Coop, RL 2003. Is the allocation of metabolisable protein prioritised to milk production rather than to immune functions in Teladorsagia circumcincta-infected lactating ewes? International Journal for Parasitology 33, 327–338.CrossRefGoogle ScholarPubMed
Hubert, J and Kerboeuf, D 1992. A microlarval development assay for the detection of anthelmintic resistance in sheep nematodes. Veterinary Record 130, 442–446.CrossRefGoogle ScholarPubMed
INRA 2015. Equine nutrition: INRA nutrient requirements, recommended allowances and feed tables. Wageningen Academic Publisher, Wageningen, the Netherlands.Google Scholar
Kanojiva, D, Shanker, D, Sudan, V, Jaiswal, AK and Parashar, R 2015. In vitro and in vivo efficacy of extracts of leaves of Eucalyptus globulus on ovine gastrointestinal nematodes. Parasitology Research 114, 141–148.Google Scholar
Kuzmina, TA, Kharchenko, VA, Starovir, AI and Dvojnos, GM 2005. Analysis of the strongylid nematodes (Nematoda: Strongylidae) community after deworming of brood horses in Ukraine. Veterinary Parasitology 131, 283–290.CrossRefGoogle ScholarPubMed
Legendre, H, Hoste, H and Gidenne, T 2017. Nutritive value and anthelmintic effect of sainfoin pellets fed to experimentally infected growing rabbits. Animal, first published online 20 February 2017. doi 10.1017/S1751731117000209.Google Scholar
Lind, EO, Eysker, M, Nilsson, O, Uggla, A and Höglund, J 2003. Expulsion of small strongyle nematodes (cyathostomin spp) following deworming of horses on a stud farm in Sweden. Veterinary Parasitology 115, 289–299.CrossRefGoogle Scholar
Love, S, Murphy, D and Mellor, D 1999. Pathogenicity of cyathostome infection. Veterinary Parasitology 85, 113–121.Google Scholar
Min, BR, Pomroy, WE, Hart, SP and Sahlu, T 2004. The effect of short-term consumption of a forage containing condensed tannins on gastro-intestinal nematode parasite infections in grazing wether goats. Small Ruminant Research 51, 279–283.Google Scholar
Molan, AL, Waghorn, GC and McNabb, WC 2002. Effect of condensed tannins on egg hatching and larval development of Trichostrongylus colubriformis in vitro . Veterinary Record 19, 65–69.CrossRefGoogle Scholar
Molento, MB 2009. Parasite control in the age of drug resistance and changing agricultural practices. Veterinary Parasitology 163, 229–234.Google Scholar
Morais-Costa, F, Bastos, GA, Soares, ACM, Costa, EGL, Vasconcelos, VO, Oliveira, NJF, Braga, FC, Duarte, ER and Lima, WS 2016. In vitro and in vivo action of Piptadenia viridiflora (Kunth) Benth against Haemonchus contortus in sheep. Veterinary Parasitology 223, 43–49.Google Scholar
Mueller-Harvey 2006. . Review – unravelling the conundrum of tannins in animal nutrition and health. Journal of the Science of Food and Agriculture 86, 2010–2037.CrossRefGoogle Scholar
Nogueira, FA, Fonseca, FD, da Silva, RB, de Paiva Ferreira, AV, Nery, PS, Geraseev, LC and Duarte, ER 2012. In vitro and in vivo efficacy of aqueous extract of Caryocar brasiliense Camb. to control gastrointestinal nematodes in sheep. Parasitology Research 111, 325–330.Google Scholar
Novobilský, A, Stringano, E, Hayot Carbonero, C, Smith, LMJ, Enemark, HL, Mueller-Harvey, I and Thamsborg, SM 2013. In vitro effects of extracts and purified tannins of sainfoin (Onobrychis viciifolia) against two cattle nematodes. Veterinary Parasitology 196, 532–537.CrossRefGoogle ScholarPubMed
Ojeda-Robertos, NF, Manolaraki, F, Theodoridou, K, Aufrere, J, Halbwirth, H, Stich, K, Regos, I, Treutter, D, Mueller-Harvey, I and Hoste, H 2010. The anthelmintic effect of sainfoin (silage, hay, fresh) and the role of flavonoid glycosides. In 61st Annual Meeting of the European Association for Animal Production, Wageningen Academic Publishers, Wageningen, the Netherlands.Google Scholar
Paolini, V, De La Farge, F, Prevot, F, Dorchies, P and Hoste, H 2005. Effects of the repeated distribution of sainfoin hay on the resistance and the resilience of goats naturally infected with gastrointestinal nematodes. Veterinary Parasitology 127, 277–283.Google Scholar
Paolini, V, Fouraste, I and Hoste, H 2004. In vitro effects of three woody plant and sainfoin extracts on 3rd-stage larvae and adult worms of three gastrointestinal nematodes. Parasitology 129, 69–77.Google Scholar
Payne, SE, Kotze, AC, Durmic, Z and Vercoe, PE 2013. Australian plants show anthelmintic activity toward equine cyathostomins in vitro . Veterinary Parasitology 196, 153–160.Google Scholar
Peachey, LE, Pinchbeck, GL, Matthews, JB, Burden, FA, Mulugeta, G, Scantlebury, CE and Hodgkinson, JE 2015. An evidence-based approach to the evaluation of ethnoveterinary medicines against strongyle nematodes of equids. Veterinary Parasitology 210, 40–52.CrossRefGoogle Scholar
Pedersen, S, Saeed, I, Michaelsen, KF, Friis, H and Murrell, KD 2002. Impact of protein energy malnutrition on Trichuris suis infection in pigs concomitantly infected with Ascaris suum . Parasitology 124, 561–568.CrossRefGoogle ScholarPubMed
Sakkas, P, Houdijk, JGM, Athanasiadou, S and Kyriazakis, I 2012. Sensitivity of periparturient breakdown of immunity to parasites to dietary protein source. Journal of Animal Science 90, 3954–3962.Google Scholar
Valderrábano, J, Calvete, C and Uriarte, J 2010. Effect of feeding bioactive forages on infection and subsequent development of Haemonchus contortus in lamb faeces. Veterinary Parasitology 172, 89–94.Google Scholar