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Reducing the number of animals used in behavioural genetic experiments using chromosome substitution strains

Published online by Cambridge University Press:  11 January 2023

MC Laarakker ,
F Ohl  and
HA van Lith
MC Laarakker*
Affiliation:
Department of Animals, Science & Society, Division of Laboratory Animal Science, Faculty of Veterinary Medicine, Utrecht University, PO Box 80.166, 3508 TD Utrecht, The Netherlands
F Ohl
Affiliation:
Department of Animals, Science & Society, Division of Laboratory Animal Science, Faculty of Veterinary Medicine, Utrecht University, PO Box 80.166, 3508 TD Utrecht, The Netherlands
HA van Lith
Affiliation:
Department of Animals, Science & Society, Division of Laboratory Animal Science, Faculty of Veterinary Medicine, Utrecht University, PO Box 80.166, 3508 TD Utrecht, The Netherlands
*
* Contact for correspondence and requests for reprints: [email protected]
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Abstract

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Chromosome substitution strains (also called consomic lines or strains) are strains in which a single, full-length chromosome from one inbred strain — the donor strain — has been transferred onto the genetic background of a second inbred strain — the host strain. Based on the results obtained from behavioural tests with the two parental strains, the minimum number of animals from each of the host and consomic strains that are required for a successful behavioural genetic analysis can be estimated. Correct application of statistical knowledge can lead to a further reduction in the number of animals used in behavioural genetic experiments using chromosome substitution strains.

Keywords

animal welfarebehavioural geneticschromosome substitution strainmiceQTLreduction
Type
Research Article
Information
Animal Welfare , Volume 15 , Issue 1 , February 2006 , pp. 49 - 54
Copyright
© 2006 Universities Federation for Animal Welfare

References

Belknap, JK 2003 Chromosome substitution strains: some quantitative considerations for genome scans and fine mapping. Mammalian Genome 14: 723–732Google ScholarPubMed
Dunnett, CW 1955 A multiple comparison procedure for comparing several treatments with a control. Journal of the American Statistical Association 50: 1096–1121CrossRefGoogle Scholar
Ohl, F 2003 Testing for anxiety. Clinical Neuroscience Research 3: 233–238CrossRefGoogle Scholar
Ohl, F, Sillaber, I, Binder, E, Keck, ME and Holsboer, F 2001 Differential analysis of behavior and diazepam-induced alterations in C57BL/6N and BALB/c mice using the modified hole board test. Journal of Psychiatric Research 35: 147–154Google ScholarPubMed
Russell, WMS and Burch, RL 1959 (reprinted 1992) The Principles of Humane Experimental Technique. Universities Federation for Animal Welfare: Wheathampstead, UKGoogle Scholar
Singer, JB, Hill, AE, Burrage, LC, Olszens, KR, Song, J, Justice, M, O'Brien, WE, Conti, DV, Witte, JS, Lander, ES and Nadeau, JH 2004 Genetic dissection of complex traits with chromosome substitution strains of mice. Science 304: 445–448CrossRefGoogle ScholarPubMed
Singer, JB, Hill, AE, Nadeau, JH and Lander, ES 2005 Mapping quantitative trait loci for anxiety in chromosome substitution strains of mice. Genetics 169: 855–862CrossRefGoogle ScholarPubMed
Turri, MG, DeFries, JC, Henderson, ND and Flint, J 2004 Multivariate analysis of quantitative trait loci influencing variation in anxiety-related behaviour in laboratory mice. Mammalian Genome 15: 69–76CrossRefGoogle ScholarPubMed
van Lith, HA 2005 Mapping of quantitative trait loci underlying strain differences in treatment susceptibility in rodents. http://www.vet.uu.nl/site/viavet_english/viavet_skeleton/Framework/main_frame_dtml?path=viavet_english/faculty/departments&skeleton_url=http://www.vet.uu.nl/site/viavet_english/viavet_skeleton (accessed 2 November 2005)Google Scholar