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Evaluation of a novel rodenticide: welfare assessment of fatal methaemoglobinaemia in adult rats (Rattus norvegicus)

Published online by Cambridge University Press:  01 January 2023

TJ Gibson*
Affiliation:
Department of Production and Population Health, Royal Veterinary College, University of London, UK
RJ Quy
Affiliation:
Animal and Plant Health Agency, York, UK
CT Eason
Affiliation:
Lincoln University and Connovation Research Ltd, Auckland, New Zealand
NG Gregory
Affiliation:
Department of Production and Population Health, Royal Veterinary College, University of London, UK
*
* Contact for correspondence and requests for reprints: [email protected]
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Abstract

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This study assessed the welfare of rats (Rattus norvegicus) poisoned with a lethal dose of the methaemoglobin (MetHb) inducing compound para-aminovalerophenone (PAVP). Twenty rats were orally gavaged with either PAVP (treated) or the vehicle only (control). Spontaneous and evoked behaviours were recorded and blood samples collected post mortem for analysis of MetHb%. Female and male rats received a mean (± SEM) dose of 263 (± 3) and 199 (± 6) mg PAVP kg−1, respectively. Mean (± SEM) time to death was 67 (± 16) and 354 (± 71) min for female and male rats, respectively. Control animals did not show any signs of intoxication. The time to death from methaemoglobinaemia in rats was significantly shorter than that reported for anticoagulants and there were no obvious signs of distress or pain.

Type
Research Article
Copyright
© 2015 Universities Federation for Animal Welfare

References

Anonymous 2010 Assessing the humaneness and efficacy of a new feral pig bait in domestic pigs. Institute of Medical and Veterinary Science. Report for the Australian Government Department of the Enviornment, Water, Heritage and the Arts: Canberra, AustraliaGoogle Scholar
Blackie, HM, MacKay, JWB, Allen, WJ, Smith, DHV, Barrett, B, Whyte, BI, Murphy, EC, Ross, J, Shapiro, L, Ogilvie, S, Sam, S, MacMorran, D, Inder, S and Eason, CT 2014 Innovative developments for long-term mammalian pest control. Pest Management Science 70: 345351. http://dx.doi.org/10.1002/ps.3627CrossRefGoogle ScholarPubMed
Bright, JE, Woodman, AC, Marrs, TC and Wood, SG 1987 Sex differences in the production of methaemoglobinaemia by 4-aminopropiophenone. Xenobiotica 17: 7983. http://dx.doi.org/10.3109/00498258709047177CrossRefGoogle ScholarPubMed
Chadha, RK, Bryce, F, Lawrence, JF, Conacher, HB and Arnold, D 1991 Toxicity of potassium cyanide added to fresh fruit and juice. Food and Chemical Toxicology 29: 681684. http://dx.doi.org/10.1016/0278-6915(91)90125-QCrossRefGoogle ScholarPubMed
Coleman, MD, Winn, MJ, Breckenridge, AM and Park, BK 1990 Sex-dependent sensitivity to dapsone-induced methaemo-globinaemia in the rat. Biochemical Pharmacology 39: 805809. http://dx.doi.org/10.1016/0006-2952(90)90165-HCrossRefGoogle ScholarPubMed
Dilks, P, Shapiro, L, Greene, T, Kavermann, MJ, Eason, CT and Murphy, EC 2011 Field evaluation of para-aminopropiophe-none (PAPP) for controlling stoats (Mustela erminea) in New Zealand. New Zealand Journal of Zoology 38: 143150. http://dx.doi.org/10.1080/03014223.2010.537668CrossRefGoogle Scholar
Eason, CT, Henderson, R, Murphy, E, Shapiro, L, MacMorran, D, Blackie, H, Brimble, M, Conole, D, Rennison, D, Gibson, TJ and Gregory, NG 2011 Retrieving and retaining older and advancing novel rodenticides-as alternatives to anticoagu-lants. Julius Kühn Institut: Bundesforschungsinstitut für Kulturpflanzen, GermanyGoogle Scholar
Eason, CT, Miller, A, MacMorran, DB and Murphy, EC 2014 Toxicology and ecotoxicology of para-aminopropiophenone (PAPP): a new predator control tool for stoats and feral cats in New Zealand. New Zealand Journal of Ecology 38: 177188Google Scholar
Eason, CT, Murphy, EC, Hix, S, and Macmorran, DB 2010 Development of a new humane toxin for predator control in New Zealand. Integrative Zoology 5: 3136. http://dx.doi.org/10.1111/j.1749-4877.2010.00183.xCrossRefGoogle ScholarPubMed
Eason, CT, Murphy, EC, Wright, GRG and Spurr, EB 2002 Assessment of risks of brodifacoum to non-target birds and mam-mals in New Zealand. Ecotoxicology 11: 3548. http://dx.doi.org/10.1023/A:1013793029831CrossRefGoogle ScholarPubMed
Edwards, RJ and Ujma, J 1995 Extreme methaemoglobinaemia secondary to recreational use of amyl nitrite. Journal of Accident & Emergency Medicine 12: 138142. http://dx.doi.org/10.1136/emj.12.2.138CrossRefGoogle ScholarPubMed
Fisher, P, Beaulsoeil, NJ, Warburton, B and Mellor, DJ 2010 How humane are our pest control tools? MAF Biosecurity New Zealand Technical Paper No 2011/01. Ministry of Agriculture and Forestry: Wellington, New ZealandGoogle Scholar
Fisher, P and O’Connor, C 2007 Oral toxicity of p-aminopro-piophenone to ferrets. Wildlife Research 34: 1924. http://dx.doi.org/10.1071/WR06125CrossRefGoogle Scholar
Fisher, P, O’Connor, CE and Morriss, G 2008 Oral toxicity of p-aminopropiophenone to brushtail possums (Trichosurus vulpecu-la), dama wallabies (Macropus eugenii), and mallards (Anas platyrhynchos). Journal of Wildlilfe Diseases 44: 655663. http://dx.doi.org/10.7589/0090-3558-44.3.655CrossRefGoogle ScholarPubMed
Fisher, PM, O’Connor, CE and Murphy, EC 2005 Acute oral toxicity of p-aminopropiophenone to stoats (Mustela erminea). New Zealand Journal of Zoology 32: 163169. http://dx.doi.org/10.1080/03014223.2005.9518409CrossRefGoogle Scholar
Gould, EM and Holmes, SJ 2008 The effect of dextromethor-phan in preventing cholecalciferol-induced poison shyness and sickness-induced anorexia in the laboratory Norway rat. Pest Management Science 64: 197202. http://dx.doi.org/10.1002/ps.1468CrossRefGoogle ScholarPubMed
Gowans, WJ 1990 Fatal methaemoglobinaemia in a dental nurse. A case of sodium nitrite poisoning. British Journal of General Practice 40: 470471Google Scholar
Greaves, JH, Redfern, R and Tinworth, H 1974 Laboratory tests of 5-Para-Chlorophenyl Silatrane as a rodenticide. Journal of Hygiene 73: 3943. http://dx.doi.org/10.1017/S0022172400023810CrossRefGoogle ScholarPubMed
Gregory, NG 2004a Poisoning. Physiology and Behaviour of Animal Suffering pp 203206. Blackwell Science: Oxford, UK. http://dx.doi.org/10.1002/9780470752494CrossRefGoogle Scholar
Gregory, NG 2004b Respiratory system. Physiology and Behaviour of Animal Sufferieng pp 215217. Blackwell Science: Oxford, UK. http://dx.doi.org/10.1002/9780470752494CrossRefGoogle Scholar
Hagler, L, Coppes, RI Jr and Herman, RH 1979 Metmyoglobin reductase. Identification and purification of a reduced nicoti-namide adenine dinucleotide-dependent enzyme from bovine heart which reduces metmyoglobin. Journal of Biological Chemistry 254: 65056514CrossRefGoogle Scholar
Hall, AH, Kulig, KW and Rumack, BH 1986 Drug- and chem-ical-induced methaemoglobinaemia. Clinical features and manage-ment. Medical Toxicology and Adverse Drug Experience 1: 253260Google Scholar
Harvey, M, Cave, G and Chanwai, G 2010 Fatal methaemoglo-binaemia induced by self-poisoning with sodium nitrite. Emergency Medicine Australasia 22: 463465. http://dx.doi.org/10.1111/j.1742-6723.2010.01335.xCrossRefGoogle Scholar
Kohn, MC, Melnick, RL, Ye, F and Portier, CJ 2002 Pharmacokinetics of sodium nitrite-induced methemoglobinemia in the rat. Drug Metabolism Disposition 30: 676683. http://dx.doi.org/10.1124/dmd.30.6.676CrossRefGoogle ScholarPubMed
Kornerup, A and Wanscher, JH 1978 Methuen Handbook of Colour. Eyre Methuen: London, UKGoogle Scholar
Langford, KH, Reid, M and Thomas, KV 2013 The occur-rence of second generation anticoagulant rodenticides in non-target raptor species in Norway. Science of The Total Environment 450-451: 205-208. http://dx.doi.org/10.1016/j.sci-totenv.2013.01.100CrossRefGoogle Scholar
Littin, KE, O’Connor, CE and Eason, CT 2000 Comparative effects of brodifacoum on rats and possums. New Zealand Plant Protection 53: 310315CrossRefGoogle Scholar
Mason, G and Littin, KE 2003 The humaneness of rodent pest control. Animal Welfare 12: 137Google Scholar
Matteucci, O, Diletti, G, Prencipe, V, Di Giannatale, E, Marconi, MM and Migliorati, G 2008 Two cases of methemo-globinaemia caused by suspected sodium nitrite poisoning. Veterinaria Italiana 44: 439453Google ScholarPubMed
Mayer, J 2007 Use of behavior analysis to recognize pain in small mammals. Lab Animal 36: 4348. http://dx.doi.org/10.1038/laban0607-43CrossRefGoogle ScholarPubMed
Murphy, EC, Eason, CT, Hix, S and MacMorran, DB 2007 Developing a new toxin for potential control of feral cats, stoats, and wild dogs in new zealand, In: Witmer, GW, Pitt, WC and Fagerstone, KA (eds) Managing Vertebrate Invasive Species pp 469473. National Wildlife Research Center: Fort Collins, USAGoogle Scholar
Niel, L and Weary, DM 2007 Rats avoid exposure to carbon dioxide and argon. Applied Animal Behaviour Science 107: 100109. http://dx.doi.org/10.1016/j.applanim.2006.08.002CrossRefGoogle Scholar
O’Connor, CE and Matthews, LR 1997 Duration of cyanide-induced conditioned food aversions in possums. Physiology & Behavior 62: 931933. http://dx.doi.org/10.1016/S0031-9384(97)00175-3CrossRefGoogle ScholarPubMed
Pan, HP, Savarie, PJ, Elias, DJ and Felton, RR 1983 Alkyl chain-length and acute oral toxicity of para-aminophenones. General Pharmacology 14: 465467. http://dx.doi.org/10.1016/0306-3623(83)90032-0CrossRefGoogle Scholar
PSD 1997 Assessment of Humaneness of Vertebrate Control Agents -Evaluation of Fully Approved or Provisionally Approved Products. Pesticides Safety Directorate: York, UKGoogle Scholar
Quy, RJ, Gibson, TJ, Lambert, MS, Eason, CT and Gregory, NG 2015 Evaluation of a novel rodenticide: acute sub-lethal effects of a methaemoglobin-inducing agent. Animal Welfare 24:427436. http://dx.doi.org/10.7120/09627286.24.4.427CrossRefGoogle Scholar
Raj, ABM and Gregory, NG 1995 Welfare implications of the gas stunning of pigs 1. Determination of aversion to the initial inhalation of carbon dioxide or argon. Animal Welfare 4: 273280Google Scholar
Rennison, D, Conole, D, Tingle, MD, Yang, J, Eason, CT and Brimble, MA 2013 Synthesis and methemoglobinemia-inducing properties of analogues of para-aminopropiophenone designed as humane rodenticides. Bioorganic & Medicinal Chemistry Letters 23:66296635. http://dx.doi.org/10.1016/j.bmcl.2013.10.046CrossRefGoogle ScholarPubMed
Rowsell, HD, Ritcey, J and Cox, F 1979 Assessment of humane-ness of vertebrate pesticides Proceedings of the Canadian Association for Laboratory Animal Science 1978-1979 pp 159249.25-28 June 1979, Calgary, CanadaGoogle Scholar
Sachdeva, R, Pugeda, JG, Casale, LR, Meizlish, JL and Zarich, SW 2003 Benzoicaine-induced methemoglobinemia. A potential-ly fatal complication of transesophageal echocardiography. Texas Heart Institute Journal 30: 308310Google ScholarPubMed
Sanchez-Barbudo, IS, Camarero, PR and Mateo, R 2012 Primary and secondary poisoning by anticoagulant rodenticides of non-target animals in Spain. Science of The Total Environment 420:280288. http://dx.doi.org/10.1016/j.scitotenv.2012.01.028CrossRefGoogle ScholarPubMed
Scawin, JW, Swanston, DW and Marrs, TC 1984 The acute oral and intravenous toxicity of p-aminopropiophenone (PAPP) to laboratory rodents. Toxicology Letters 23: 359365. http://dx.doi.org/10.1016/0378-4274(84)90034-1CrossRefGoogle ScholarPubMed
Sridhara, S and Srihari, K 1980 Bait shyness towards zinc phos-phide and vacor in the larger bandicoot rat bandicota-indica (Bechstein). Indian Journal of Experimental Biology 18: 10291031Google ScholarPubMed
Stolk, JM and Smith, RP 1966 Species differences in methemo-globin reductase activity. Biochemical Pharmacology 15: 343351. http://dx.doi.org/10.1016/0006-2952(66)90305-4CrossRefGoogle Scholar
Vanderbelt, JM, Pfeiffer, C, Kaiser, M and Sibert, M 1944 Methemoglobinemia after administration of p-aminoacetophe-none and p-aminopropiophenone. Journal of Pharmacology and Experimental Therapeutics 80: 3138Google Scholar