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Effect of Transport Stress on Serum Haptoglobin and Pig-Map in Pigs

Published online by Cambridge University Press:  11 January 2023

Y Saco ,
M J Docampo ,
E Fàbrega ,
X Manteca ,
A Diestre ,
F Lampreave  and
A Bassols
Y Saco
Affiliation:
Departament de Bioquímica i Biologia Molecular i Servei de Bioquímica Clínica Veterinaria, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
M J Docampo
Affiliation:
Departament de Bioquímica i Biologia Molecular i Servei de Bioquímica Clínica Veterinaria, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
E Fàbrega
Affiliation:
Departament de Biología Cel.lular, Fisiología i Immunologia, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
X Manteca
Affiliation:
Departament de Biología Cel.lular, Fisiología i Immunologia, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
A Diestre
Affiliation:
Institut de Recerca i Tecnologia Agroalimentàries, Centre de Tecnologia de la Carn, Granja Camps i Armet, 17121 Monells, Girona, Spain
F Lampreave
Affiliation:
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Zaragoza, Spain
A Bassols*
Affiliation:
Departament de Bioquímica i Biologia Molecular i Servei de Bioquímica Clínica Veterinaria, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
*
# Contact for correspondence and requests for reprints: [email protected]
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Abstract

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This study was undertaken in order to determine the variation of the acute phase proteins haptoglobin and Pig-MAP (major acute phase protein) in the serum of pigs affected by transport-related stress. Pigs were subjected to one of two pre-slaughter treatments: a) short-duration transport (1 h 15 min transport and 2 h lairage); or b) long-duration transport (6 h transport and 14 h lairage). There were 10 individuals in each treatment group, belonging to the NN (n = 5) or Nn (n = 5) genotypes for halothane susceptibility. Samples were taken before transport, just after transport, and on stunning at slaughter. We measured levels of serum haptoglobin, Pig-MAP and cortisol. Our results showed that the short-duration transport did not modify the levels of haptoglobin or of Pig-MAP in any of the three samples, whereas cortisol was increased just after transport. In contrast, there was an increase in haptoglobin and Pig-MAP in serum from animals after long-duration transport, as observed in the post-mortem samples (20-21 h after the beginning of transport); cortisol levels were not increased in these conditions. In this experiment, homozygotes for the halothane gene tended to have higher values of haptoglobin after slaughter than did heterozygotes. In conclusion, combined determination of acute phase proteins and cortisol levels could provide valuable information on welfare problems related to transport.

Keywords

acute phase proteinsanimal welfarehaptoglobinPig-MAPpigstransport stress
Type
Short Communication
Information
Animal Welfare , Volume 12 , Issue 3 , August 2003 , pp. 403 - 409
Copyright
© 2003 Universities Federation for Animal Welfare

References

Eckersall, P D, Saini, P K and McComb, C 1996 The acute-phase response of acid-soluble glycoprotein, alpha(1)-acid glycoprotein, ceruloplasmin, haptoglobin and C-reactive protein, in the pig. Veterinary Immunology and Immunopathology 51: 377–385CrossRefGoogle ScholarPubMed
Eurell, T E, Bane, D P, Hall, W F and Schaeffer, D J 1992 Serum haptoglobin concentration as an indicator of weight gain in pigs. Canadian Journal of Veterinary Research 56: 6–9Google ScholarPubMed
Fàbrega, E, Diestre, A, Carrión, D, Font, J and Manteca, X 2002 Impact of the halothane gene on pre-slaughter mortality in two Spanish commercial pig abattoirs. Animal Welfare 11: 449–452Google Scholar
Fujii, J, Otsu, K, Zorzato, F, De Leon, S, Khanna, V K, Weilter, J E, O'Brien, P J and MacLennan, D H 1991 Identification of a mutation in porcine ryanodine receptor associated with malignant hyperthermia. Science 253: 448–451CrossRefGoogle ScholarPubMed
González-Ramón, N, Alava, M A, Sarsa, J A, Piñeiro, M, Escartín, A, Garcia-Gil, A, Lampreave, F and Piñeiro, A 1995 The major acute phase serum protein in pigs is homologous to human plasma kallikrein sensitive PK-120. FEBS Letters 371: 227–230Google ScholarPubMed
González-Ramón, N, Hoebe, K, Alava, M A, van Leengoed, L, Lampreave, F and Piñeiro, A 2000 Pig MAP/ITIH4 and haptoglobin are interleukin-6 dependent acute-phase plasma proteins in porcine primary cultured hepatocytes. European Journal of Biochemistry 267: 1–9CrossRefGoogle ScholarPubMed
Grandin, T and Deesing, M J 1998 Genetics and behaviour during handling, restraint and herding. In: Grandin, T (ed) Genetics and the Behaviour of Domestic Animals pp 113–145. Academic Press: San Diego, USAGoogle Scholar
Gruys, E, Obwolo, M J and Toussaint, M J M 1994 Diagnostic significance of the major acute phase proteins in veterinary clinical chemistry: a review. Veterinary Bulletin 64: 1009–1018Google Scholar
Hall, W F, Eurell, T E, Hansen, R D and Herr, L 1992 Serum haptoglobin concentration in swine naturally or experimentally infected with Actinobacillus pleuropneumoniae. Journal of the American Veterinary Medical Association 201: 1730–1733Google ScholarPubMed
Heegaard, P M H, Klausen, J, Nielsen, J P, González-Ramón, N, Piñeiro, M, Lampreave, F and Alava, M 1998 The porcine acute phase response to infection with Actinobacillus pleuropneumoniae. Haptoglobin, C-reactive protein, major acute phase protein and serum amyloid A protein are sensitive indicators of infection. Comparative Biochemistry and Physiology 119B: 365–373CrossRefGoogle Scholar
Kaneko, J J 1997 Serum proteins and the dysproteinemias. In: Kaneko, J J, Harvey, J W and Bruss, M L (eds) Clinical Biochemistry of Domestic Animals pp 132–134. Academic Press: New York, USAGoogle Scholar
Kent, J 1992 Acute phase proteins: their use in veterinary diagnosis. British Veterinary Journal 148: 279–282CrossRefGoogle ScholarPubMed
Lampreave, F, González-Ramón, N, Martínez-Ayensa, S, Hernández, M A, Lorenzo, H K, García-Gil, A and Piñeiro, A 1994 Characterisation of the acute phase serum protein response in pigs. Electrophoresis 15: 672–676CrossRefGoogle ScholarPubMed
Murata, H and Miyamoto, T 1993 Bovine haptoglobin as a possible immunomodulator in the sera of transported calves. British Veterinary Journal 149: 277–283CrossRefGoogle ScholarPubMed
Murray, A C and Johnson, C P 1998 Impact of halothane gene on muscle quality and pre-slaughter deaths in Western Canadian pigs. Canadian Journal of Animal Science 78: 543–548CrossRefGoogle Scholar
Piñeiro, C, Lorenzo, E, Piñeiro, M, Lampreave, F and Alava, M A 2001a Effect of transport and farm sanitary conditions in the concentration of acute phase plasma proteins Pig-MAP and haptoglobin. Proceedings of the Second European Colloquium on Animal Acute Phase Proteins p 6. University of Bonn: Bonn, GermanyGoogle Scholar
Piñeiro, M, Alava, M A, Lorenzo, E, Piñeiro, C, Piñeiro, A and Lampreave, F 2001b Characterisation of the acute phase protein response in pigs affected by transport related stress. Proceedings of the Second European Colloquium on Animal Acute Phase Proteins p 5. University of Bonn: Bonn, GermanyGoogle Scholar
Ruis, M A W, Te Brake, J H P, Engel, B, Ekkel, E D, Buist, W G, Blokhuis, H J and Koolhaas, J M 1997 The circadian rhythm of salivary cortisol in growing pigs: effects of age, gender and stress. Physiology & Behavior 62: 623–630CrossRefGoogle ScholarPubMed
Toussaint, M J M, Van Ederen, A M and Gruys, E 1995 Implication of clinical pathology in assessment of animal health and in animal production and meat inspection. Comparative Haematology International 5: 149–157CrossRefGoogle Scholar
Toussaint, M J M, Lipperheide, C, Eckersall, P D, Alava, M, Jobert, J L, Heegard, P M H, Meloen, R H and Madec, F 2000 Assessment of health in pigs by acute phase protein assays. Proceedings of the Tenth International Congress on Animal Hygiene, Vol I pp 139–143. Animal Health Service: Boxtel, The NetherlandsGoogle Scholar
Von Borrell, E and Ladewig, J 1992 Relationship between behaviour and adreno-cortical response pattern in domestic pigs. Applied Animal Behaviour Science 34: 299–309CrossRefGoogle Scholar