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Vitamin A regulation of stearoyl-CoA desaturase mRNA levels and fatty acid composition in sheep tissues

Published online by Cambridge University Press:  18 August 2016

Z.C.T.R. Daniel ,
A.M. Salter  and
P.J. Buttery
Z.C.T.R. Daniel*
Affiliation:
Division of Nutritional Biochemistry, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK
A.M. Salter
Affiliation:
Division of Nutritional Biochemistry, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK
P.J. Buttery
Affiliation:
Division of Nutritional Biochemistry, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK
*
†E-mail:[email protected]
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Abstract

The effect of vitamin A (retinol) on ovine stearoyl-CoA desaturase (SCD) mRNA levels and fatty acid composition was investigated. Sheep adipose tissue explants were maintained in culture for 24 h in the presence of all-trans retinoic acid (RA). Tissue SCD mRNA levels were increased with 25 μmol/l RA but the levels of SCD mRNA in tissue treated with 100 μmol/l RA were not different from control. The effect of vitamin A supplement on SCD mRNA levels in vivo was then characterized: growing lambs were given a concentrate diet (2 kg/day) containing 0, 0.225, 1.125 and 3.375 mg vitamin A per kg diet for 21 days. Treatment resulted in a concentration-dependent increase in adipose tissue and liver SCD mRNA levels, although the greatest effect was seen in the liver. SCD mRNA levels were highest in tissue from animals given 0.225 mg vitamin A per kg diet and further increases in vitamin A supplementation were not accompanied by corresponding increases in SCD gene expression. Fatty acid composition was also determined. Overall, tissue from animals given vitamin A had greater levels of both palmitoleic and oleic acid relative to their precursors. These data clearly show that SCD gene expression in adipose tissue and liver is regulated by retinoic acid and the liver appears to be most responsive. However, although significant, the change in the proportion of oleic acid was only small indicating that dietary manipulation with vitamin A is not a suitable method for increasing the unsaturated fat content of sheep meat.

Keywords

oleic acidretinolsheepstearoyl-CoA desaturase
Type
Growth, development and meat science
Information
Animal Science , Volume 78 , Issue 2 , April 2004 , pp. 237 - 243
Copyright
Copyright © British Society of Animal Science 2004

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References

Barasi, M. E. 1997. Human nutrition: health perspective. Arnold, London.Google Scholar
Barber, M. C., Ward, R. J., Richards, S. E., Salter, A. M., Buttery, P. J., Vernon, R. G. and Travers, M. T. 2000. Ovine adipose tissue monounsaturated fat content is correlated to depot-specific expression of the stearoyl-CoA desaturase gene. Journal of Animal Science 78: 62–68.Google Scholar
Casimir, D. A., Miller, C. W. and Ntambi, J. M. 1996. Preadipocyte differentiation blocked by prostaglandin stimulation of prostanoid FP-2 receptor in murine 3T3-L1 cells. Differentiation 60: 203–210.Google Scholar
Choi, Y. J., Kim, Y. C., Han, Y. B., Park, Y., Pariza, M. W. and Ntambi, J. M. 2000. The trans-10, cis-12 isomer of conjugated linoleic acid down regulates stearoyl-CoA desaturase 1 gene expression in 3T3-L1 adipocytes. Journal of Nutrition 130: 1920–1924.CrossRefGoogle Scholar
Chomcynski, P. and Sacchi, N. 1987. Single step method of RNA isolation by acid guanidinium thiocyanate phenol chloroform extraction. Analytical Biochemistry 162: 156–159.Google Scholar
Christie, W. W., Sebedio, J. L. and Juaneda, P. 2001. A practical guide to the analysis of conjugated linoleic acid. Inform 12: 147–152.Google Scholar
Daniel, Z. C. T. R., Richards, S. E., Salter, A. M. and Buttery, P. J. 2004. Insulin and dexamethasone regulate stearoyl-CoA desaturase mRNA levels and fatty acid synthesis in ovine adipose tissue explants. Journal of Animal Science 82: 231–237.CrossRefGoogle ScholarPubMed
Department of Health. 1994. Nutritional aspects of cardiovascular disease. Report on health and social subjects (46), Her Majesty’s Stationery Office Publications, London.Google Scholar
Enoch, H. G., Catala, A. and Strittmater, P. 1976. Mechanism of rat liver microsomal stearoyl-CoA desaturase. Journal of Biological Chemistry 251: 5095–5103.Google Scholar
Enser, M., Hallett, K., Hewitt, B., Fursey, G. A. and Wood, J. D. 1996. Fatty acid content and consumption of English beef, lamb and pork at retail. Meat Science 42: 443–456.Google Scholar
European Agency for the Evaluation of Medicinal Products. 1998. Veterinary medicines and inspections. Committee for Veterinary Medicinal Products — Vitamin A summary report. EMEA/MRL/365/98-FINAL.Google Scholar
Folch, J., Lee, M. and Sloan Stanley, G. H. 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226: 497–509.Google Scholar
Kaestner, K. H., Ntambi, J. M., Kelly, T. J. and Lane, M. D. 1989. Differentiation-induced gene expression in 3T3-L1 preadipocytes: a second differentially expressed gene encoding stearoyl-CoA desaturase. Journal of Biological Chemistry 264: 14755–14761.Google Scholar
Kramer, J. K. G., Fellner, V., Dugan, M. E. R., Sauer, F. D., Mossoba, M. M. and Yurawecz, M. P. 1997. Evaluating acid and base catalysts in the methylation of milk and rumen fatty acids with special emphasis on conjugated dienes and total trans fatty acids. Lipids 32: 1210–1228.Google Scholar
Landschulz, K. T., Jump, D. B., Macdougald, O. A. and Lane, M. D. 1994. Transcriptional control of the stearoyl CoA desaturase 1 gene by polyunsaturated fatty acids. Biochemical and Biophysical Research Communications 200: 763–768.Google Scholar
Lawes Agricultural Trust. 20002. Genstat release 6.1 for Windows . Rothamsted Experimental Station, Harpenden.Google Scholar
Lee, K. N., Pariza, M. W. and Ntambi, J. M. 1998. Conjugated linoleic acid decreases hepatic stearoyl-CoA desaturase mRNA expression. Biochemical and Biophysical Research Communications 248: 817–821.CrossRefGoogle ScholarPubMed
McDowell, L.R. 1989. Vitamins in animal nutrition. Academic Press Inc., London.Google Scholar
Miller, C. W., Waters, K. M. and Ntambi, J. M. 1997. Regulation of hepatic stearoyl-CoA desaturase gene 1 by vitamin A. Biochemical and Biophysical Research Communications 231: 206–210.Google Scholar
Ntambi, J. M. 1992. Dietary regulation of stearoyl-CoA desaturase 1 gene expression in mouse liver. Journal of Biological Chemistry 267: 10925–10930.Google Scholar
Ntambi, J. M., Buhrow, S. A., Kaestner, K. H., Christy, R. J., Sibley, E., Kelly, T. J. and Lane, M. D. 1988. Differentiation-induced gene expression in 3T3-L1 preadipocytes: characterization of a differentially expressed gene encoding stearoyl-CoA desaturase. Journal of Biological Chemistry 263: 17291–17300.Google Scholar
Robertson, J. P., Faulkner, A. and Vernon, R. G. 1982. Regulation of glycolysis and fatty acid synthesis from glucose in sheep adipose tissue. Biochemical Journal 206: 577–586.Google Scholar
Samuel, W., Kutty, R. K., Nagineni, S., Gordon, J. S., Prouty, S. M., Chandraratna, R. A. S. and Wiggert, B. 2001. Regulation of stearoyl coenzyme A desaturase expression in human retinal pigment epithelia cells by retinoic acid. Journal of Biological Chemistry 276: 28744–28750.Google Scholar
Tebbey, P. W. and Buttke, T. M. 1992. Arachidonic acid regulates unsaturated fatty acid synthesis in lymphocytes by inhibiting stearoyl-CoA desaturase gene expression. Biochimica et Biophysica Acta 1171: 27–34.CrossRefGoogle ScholarPubMed
Ward, R. J., Travers, M. T., Richards, S.E., Vernon, R. G., Salter, A. M., Buttery, P. J. and Barber, M. C. 1998. Stearoyl-CoA desaturase mRNA is transcribed from a single gene in the ovine genome. Biochimica et Biophysica Acta 1391: 145–156.CrossRefGoogle ScholarPubMed
Waters, K. and Ntambi, J. M. 1994. Insulin and dietary fructose induce stearoyl-CoA desaturase 1 gene expression in liver of diabetic mice. Journal of Biological Chemistry 269: 27773–27777.Google Scholar
Westerling, D. B. and Hedrick, H. B. 1979. Fatty acid composition of bovine lipids as influenced by diet, sex and anatomical location and relationship to sensory characteristics. Journal of Animal Science 48: 1343–1348.Google Scholar
Zhang, L., Ge, L., Parimoo, D., Stenn, K. and Prouty, S. M. 1999. Human stearoyl-CoA desaturase: alternative transcripts generated from a single gene by usage of tandem polyadenylation sites. Biochemical Journal 340: 255–264.Google Scholar