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Validation of use of purine bases as a microbial marker by 15N labelling in growing lambs given high-concentrate diets: effects of grain processing, animal age and digesta sampling site

Published online by Cambridge University Press:  09 March 2007

A. R. Askar ,
J. A. Guada ,
J. Balcells ,
A. de Vega  and
C. Castrillo
A. R. Askar
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain
J. A. Guada*
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain
J. Balcells
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain
A. de Vega
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain
C. Castrillo
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain
*
‡E-mail: [email protected]
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Abstract

The origin of post-ruminal purine bases (PB) was studied in 24 growing lambs that were given a pelleted concentrate plus barley straw (C) or whole barley grain plus protein supplement (WB). Six lambs from each treatment were slaughtered at 10 and 30 days post weaning after 15N labelling of microbial nitrogen (N) and PB. Microbial contribution to digesta non-ammonia N (NAN) and PB was lower (P < 0·01) when estimated from duodenal rather than abomasal samples (0·36 v. 0·52 (s.e.d. 0·021) for NAN and 0·47 v. 0·77 (s.e.d. 0·029) for PB) as a result of endogenous contamination. In comparison with 15N, total PB/N led to higher estimates (P < 0·01) of microbial contribution to abomasal NAN in WB treatment (0·62 v. 0·46 s.e.d. 0·049). The difference was removed after correcting for microbial PB, while this effect was not observed with < the C diet, resulting in a marker by diet interaction (P < 0·05). Abomasal PB flow increased (P < 0·1) from 10 to 30 days after weaning mainly due to the higher proportion of microbial PB (0·70 v. 0·81 (s.e.d. 0·047)). Rumen apparent PB degradation did not differ between diets in older lambs, but it was proportionally 0·39 lower for WB treatment (P < 0·05) in younger lambs. When the microbial PB flow was estimated indirectly from labelled microbial N and the PB/N ratio of bacterial extracts the estimates were in agreement with those derived from PB-15N in the WB treatment but resulted in unrealistic values in lambs on diet C. Results suggest that significant proportions of dietary PB can escape rumen degradation which may lead to overestimation of microbial contribution to abomasal NAN when the PB/N ratio is used as marker. The extent of the overestimation is affected by the lamb age and grain processing.

Keywords

barleylambsmicrobial markers15Npurines
Type
Research Article
Information
Animal Science , Volume 81 , Issue 1 , August 2005 , pp. 57 - 65
Copyright
Copyright © British Society of Animal Science 2005

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Footnotes

†Animal and Poultry Nutrition Department, Desert Research Center, El-Matareya, Cairo, Egypt.

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