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Size distribution of bovine steroidogenic luteal cells during pregnancy

Published online by Cambridge University Press:  18 August 2016

Ş Arikan  and
A. Yigit
Ş Arikan*
Affiliation:
University of Kirikkale, Faculty of Veterinary Medicine, 71100 Kirikkale, Turkey
A. Yigit
Affiliation:
University of Kirikkale, Faculty of Veterinary Medicine, 71100 Kirikkale, Turkey
*
†E-mail:[email protected]
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Abstract

This study was designed to investigate the size distribution of bovine steroidogenic luteal cells throughout pregnancy. Corpora lutea collected from three different stages of pregnancy were used. Luteal tissue was dissociated into single-cell suspension by enzyme treatments. Cells were stained for 3β-hydroxysteroid dehydrogenase (HSD) activity a marker for steroidogenic cells. The steroidogenic cells covered a wide spectrum of size ranging from 10 to 60 µm in diameter. There was a significant increase in mean cell diameter (P > 0·05) as pregnancy progressed. Mean diameter of 3β-HSD positive cells increased from 17·03 (s.e. 1·3) µm in the corpus luteum of early pregnancy to 33·38 (s.e. 2·4) µm in the corpus luteum of advanced pregnancy. The ratio of large (>22 µm in diameter) to small (10 to 22 µm in diameter) luteal cells was 0·32 : 1·0 in the early pregnancy, with the 10 to 22 µm cell size class predominant. However, the ratio of large to small luteal cells was increased to 6·49 : 1·0 µm as pregnancy advanced and 23 to 42 µm cell sizes become predominant. It is likely that small luteal cells develop into large cells as gestation progresses. Development of pregnancy is associated with an increase in size of steroidogenic luteal cells.

Keywords

corpus luteum, cowshydroxysteroid dehydrogenaseluteal cellspregnancy
Type
Reproduction
Information
Animal Science , Volume 73 , Issue 2 , October 2001 , pp. 323 - 327
Copyright
Copyright © British Society of Animal Science 2001

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