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Serum somatotropin concentrations in Holstein heifers administered growth hormone-releasing factor and somatostatin

Published online by Cambridge University Press:  02 September 2010

G. W. Kazmer  and
S. A. Zinn
G. W. Kazmer
Affiliation:
Department of Animal Science, Box U–40, University of Connecticut, Storrs, CT 06269–4040, USA
S. A. Zinn
Affiliation:
Department of Animal Science, Box U–40, University of Connecticut, Storrs, CT 06269–4040, USA
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Abstract

Dose-response experiments were designed to determine the dosage of somatostatin (SRIF) necessary to alter serum growth hormone (GH) response to administration of GH-releasing factor (1·44; GRF) in cattle. The objective of this experimentation was to develop a GRF/SRIF challenge model that might be useful in early identification of animals which are genetically superior for milk production traits. In experiment 1, calves received either 0, 5 or 10 /JLg GRF per 100 kg body weight (BW) or GRF 1·29 at 1 ug per 100 kg BW. Both the 5 and 10 ug GRF per 100 kg BW dosages increased GH concentrations (P < 0·05). In experiment 2, animals received 3 ug GRF per 100 kg BW concurrent with administration of 0, 0·5, 1 or 2 ug SRIF per 100 kg BW at 0 min, with second dosage of GRF at +120 min. Administration of SRIF in those dosages did not alter GH response to GRF. In experiment 3, animals received 5 ug SRIF per 100 kg BW at -2, -1, 0 or +1 min relative to 3 ug GRF per 100 kg BW, with a second GRF injection at +120 min. No differences (P < 0·05) were found in response due to timing of SRIF administration. In experiment 4, animals were administered 0, 5, 10 or 20 Xg SRIF per 100 kg BW concurrent with 3 ug GRF per 100 kg BW. SRIF delayed (P < 0·05) the occurrence of maximum GH concentrations but did not affect the maximum concentration nor the area under the GH response curve (AUC) following either the first or second GRF injection. Utilizing data from experiment 4, individual animal response to GRF was reasonably consistent, as repeatabilities of AUCs for 1 h following first and second GRF injections were 0·80 and 0·65, respectively. Furthermore, sufficient among-animal variation existed so that animals could be distinguished from each other. Thus we conclude the method described herein might be useful in identifying superior dairy calves but accuracy would potentially be enhanced by collecting blood samples more frequently during the 20 min immediately after GRF injection.

Keywords

heiferssomatoliberinsomatotropinsomatostatin
Type
Research Article
Information
Animal Science , Volume 66 , Issue 3 , June 1998 , pp. 551 - 556
Copyright
Copyright © British Society of Animal Science 1998

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References

Results

Campbell, R. M., Lee, Y., Riviera, J., Heimer, E. P., Felix, A. M. and Mowles, T. F. 1991. GRF analogs and fragments: correlation between receptor binding, activity and structure. Peptides 12: 569–574.CrossRefGoogle ScholarPubMed
Davis, S. L. and Anfinson, M. 1975. Dose-response influence of prostaglandin Ej and somatostatin on plasma levels of growth hormone, prolactin and thyrotropin in sheep. Journal of Animal Science 41: 172–177.CrossRefGoogle Scholar
Enright, W. J., Chapin, L. T., Moseley, W. M., Zinn, S. A., Kamdar, M. B., Krabill, L. F. and Tucker, H. A. 1989. Effects of infusions of various doses of bovine growth hormone-releasing factor on blood hormones and metabolites in lactating Holstein cows. Journal of Endocrinology 122: 671–679.CrossRefGoogle ScholarPubMed
Hafs, H. D., Kiser, T. E., Haynes, N. B., Kesner, J. S. and Stellflug, J. N. 1977. Release of pituitary hormones, cortisol, testosterone and insulin in response to prostaglandin F2c (given during intracarotid infusion of somatostatin in bulls. Journal of Animal Science 44: 1061–1066.CrossRefGoogle Scholar
Harvey, S., Scanes, C. G. and Daughaday, W. H. 1995. Growth hormone. CRC Press, Boca Raton, FL.Google Scholar
Kazmer, G. W., Canfield, R. W. and Bean, B. 1991. Somatotropin and prolactin profile characteristics in proven Al dairy sires. Journal of Animal Science 69: 1601–1606.CrossRefGoogle Scholar
Kazmer, G. W., Zinn, S. A., Rycroft, H. and Campbell, R. M. 1992. Serum growth hormone in and semen characteristics of proven AI dairy sires after administration of growth hormone-releasing factor. Canadian Journal of Animal Science 72: 959–963.CrossRefGoogle Scholar
Kiddy, C. A. 1979. A review of research on genetic variation in physiological characteristics related to performance in dairy cattle. Journal of Dairy Science 62: 818–824.CrossRefGoogle ScholarPubMed
Klindt, J. 1988. Relationship between growth hormone and prolactin secretory parameter estimates in Holstein bulls and their predicted differences for lactational traits. Journal of Animal Science 66: 2784–2790.CrossRefGoogle ScholarPubMed
Lapierre, H., Petitclerc, D., Pelletier, G., Delorme, L., Dubreuil, P., Morisset, J., Gaudreau, P., Couture, Y. and Brazeau, P. 1990. Dose effect of human growth hormonereleasing factor and thyrotropin-releasing factor on hormone concentrations in lactating dairy cows. Domestic Animal Endocrinology 7: 485–496.CrossRefGoogle ScholarPubMed
McCutcheon, S. N., Bauman, D. E., Murphy, W. A., Lance, V. A. and Coy, D. H. 1984. Effect of synthetic human pancreatic growth hormone-releasing factor on plasma growth hormone concentrations in lactating cows. Journal of Dairy Science 67: 2881–2886.CrossRefGoogle ScholarPubMed
Moseley, W. M., Krabill, L. F., Friedman, A. R. and Olsen, R. F. 1984. Growth hormone response of steers injected with synthetic human pancreatic growth hormonereleasing factor. Journal of Animal Science 58: 430–435.CrossRefGoogle Scholar
Padmanabhan, V., Enright, W. J., Zinn, S. A., Convey, E. M. and Tucker, H. A. 1987. Modulation of growth hormone-releasing factor-induced release of growth hormone from bovine pituitary cells. Domestic Animal Endocrinology 4: 243–252.CrossRefGoogle ScholarPubMed
Petitclerc, D., Pelletier, G., Lapierre, H., Gaudreau, P., Couture, Y., Dubreuil, P., Morisset, J. and Brazeau, P. 1987. Dose response of two synthetic human growth hormone-releasing factors on growth hormone release in heifers and pigs. Journal of Animal Science 65: 996–1005.CrossRefGoogle Scholar
Plouzek, C. A., Molina, J. R., Hard, D. L., Vale, W. W., Rivier, J., Trenkle, A. and Anderson, L. L. 1988. Effects ofgrowth hormone-releasing factor and somatostatin on growth hormone secretion in hypophysial stalk-transected beef calves. Proceedings of the Society for Experimental Biology and Medicine 189: 158–167.CrossRefGoogle Scholar
Sartin, J. L., Pierce, A. C., Kemppainen, R. J., Bartol, F. F., Cummins, K. A., Marple, D. N. and Williams, J. C. 1989. Differential growth hormone responsiveness to glucose and growth hormone-releasing factor in lactating dairy cows. Ada Endocrinologica 120: 319–325.Google ScholarPubMed
Statistical Analysis Systems Institute. 1982. SAS user's guide: statistics. Statistical Analysis Systems Institute, Cary, NC.Google Scholar
Woolliams, J. A. and Lovendahl, P. 1991. Physiological attributes of male and juvenile cattle differing in genetic merit for milk yield: a review. Livestock Production Science 29: 1–12.CrossRefGoogle Scholar
Zinn, S. A., Kazmer, G. W., Rycroft, H. and Campbell, R. M. 1994. Growth hormone response after administration of growth hormone-releasing factor to proven dairy sires. Livestock Production Science 40: 157–164.CrossRefGoogle Scholar