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Lipid metabolism in finishing bulls and steers implanted with oestradiol-17 β-dipropionate

Published online by Cambridge University Press:  02 September 2010

R. L. Prior ,
S. B. Smith ,
B. D. Schanbacher  and
H. J. Mersmann
R. L. Prior
Affiliation:
Roman L. Hruska US Meat Animal Research Centre, US Department of Agriculture, Clay Center, Nebraska 68933, USA
S. B. Smith
Affiliation:
Roman L. Hruska US Meat Animal Research Centre, US Department of Agriculture, Clay Center, Nebraska 68933, USA
B. D. Schanbacher
Affiliation:
Roman L. Hruska US Meat Animal Research Centre, US Department of Agriculture, Clay Center, Nebraska 68933, USA
H. J. Mersmann
Affiliation:
Roman L. Hruska US Meat Animal Research Centre, US Department of Agriculture, Clay Center, Nebraska 68933, USA
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Abstract

Twenty bull calves were assigned to one of four treatment groups: 1) intact bulls; 2) intact bulls implanted with oestradiol dipropionate; 3) steers, and 4) steers implanted with oestradiol dipropionate. Subcutaneous adipose tissue was obtained by biopsy 5 months after implantation. Experimental treatment did not alter (P > 0·05) mean adipose cell volume, diameter or cell number per g adipose tissue. Soluble protein content of adipose cells from bulls was increased by implantation, but not in steers. In vitro rates of fatty acid synthesis were higher in oestrogen-implanted cattle compared with nonimplanted cattle when expressed per g adipose tissue, but not when expressed per cell. Maximal activities of the lipogenic-related enzymes, acetyl coenzyme A carboxylase, adenosine triphosphate-citrate lyase, aconitate hydratase and NADP-isocitrate dehydrogenase were higher in implanted compared with nonimplanted cattle when the activities were expressed per g tissue and acetyl coenzyme A carboxylase and aconitase were also higher when expressed per adipose cell. Bulls and steers had the same basal and stimulated lipolytic rates and implantation of oestradiol dipropionate had no effect on either basal or stimulated lipolytic rate. In this experiment, the oestrogenic implant appeared to have a stimulatory effect on in vitro estimates of lipogenesis.

Type
Research Article
Information
Animal Production , Volume 37 , Issue 1 , August 1983 , pp. 81 - 88
Copyright
Copyright © British Society of Animal Science 1983

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References

REFERENCES

Bailey, C. M., Probert, C. L. and Bohman, V. R. 1966. Growth rate, feed utilization and body composition of young bulls and steers. J. Anim. Sci. 25: 132–137.CrossRefGoogle ScholarPubMed
Bauman, D. E. 1976. Intermediary metabolism of adipose tissue. Fedn Proc. Fedn Am. Socs exp. Biol. 35: 2308–2313.Google ScholarPubMed
Champagne, J. R., Carpenter, J. W., Hentges, J. F. Jr, Palmer, A. Z. and Koger, M. 1969. Feedlot performance and carcass characteristics of young bulls and steers castrated at four ages. J. Anim. Sci. 29: 887–890.CrossRefGoogle Scholar
Dole, V. P. and Meinertz, H. 1960. Microdetermination of long-chain fatty acids in plasma and tissues. J. biol. Chem. 235: 2595–2599.CrossRefGoogle ScholarPubMed
Etherton, T. D., Bauman, D. E. and Romans, J. R. 1977. Lipolysis in subcutaneous and perirenal adipose tissue from sheep and dairy steers. J. Anim. Sci. 44: 1100–1106.CrossRefGoogle ScholarPubMed
Etherton, T. D., Thompson, E. H. and Allen, C. E. 1977. Improved techniques for studies of adipocyte cellularity and metabolism. J. Lipid Res. 18: 552–557.CrossRefGoogle ScholarPubMed
Galbraith, H. and Topps, J. H. 1981. Effects of hormones on the growth and body composition of animals. Nutr. Abstr. Rev. Ser. B. 51: 521–540.Google Scholar
Hanson, R. W. and Ballard, F. J. 1967. The relative significance of acetate and glucose as precursors for lipid synthesis in liver and adipose tissue from ruminants. Biochem. J. 105: 524–528.CrossRefGoogle ScholarPubMed
Harvey, W. R. 1975. Least squares analyses of data with unequal subclass numbers. U.S. Dep. Agric. ARS H-4.Google Scholar
Ingle, D. L., Bauman, D. E., Mellenberoer, R. W. and Johnson, D. E. 1973. Lipogenesis in the ruminant: effect of fasting and refeeding on fatty acid synthesis and enzymatic activity of sheep adipose tissue. J. Nutr. 103: 1479–1488.CrossRefGoogle ScholarPubMed
Kelly, T. F. 1965. Improved method for microtitration of fatty acids. Analyt. Chem. 37: 1078–1079.CrossRefGoogle Scholar
Klosterman, E. W., Cahill, V. R., Kunkle, L. E. and Moxon, A. L. 1955. The subcutaneous implantation of stilbestrol in fattening bulls and steers. J. Anim. Sci. 14: 1050–1058.CrossRefGoogle Scholar
Kral, J. G. and Tisell, L. E. 1976. The effects of castration on body composition, adipose tissue cellularity, and lipid and carbohydrate metabolism in adult male rats. Ada Endocr., Copnh, 81: 644–654.Google ScholarPubMed
Mears, G. J. and Mendel, V. E. 1974. Correlation of food intake in lambs with adipocyte glucose metabolism and NEFA release. J. Physiol, Lond. 240: 625–637.CrossRefGoogle ScholarPubMed
Metz, S. H. M. and Van den Bergh, S. G. 1977. Regulation of fat mobilization in adipose tissue of dairy cows in the period around parturition. Neth. J. agric. Sci. 25: 198–211.Google Scholar
Mokrasch, C. C. and McGilvery, R. W. 1956. Purification and properties of fructose-1,6-diphosphatase. J. biol. Chem. 221: 909–917.CrossRefGoogle Scholar
Ogilvie, M. L., Faltin, E. C., Hauser, E. R., Bray, R. W. and Hoekstra, W. G. 1960. Effects of stilbestrol in altering carcass composition and feed lot performance of beef steers. J. Anim. Sci. 19: 991–1001.CrossRefGoogle Scholar
Pothoven, M. A. and Beitz, D. C. 1975. Changes in fatty acid synthesis and lipogenic enzymes in adipose tissue from fasted and fasted-refed steers. J. Nutr. 105: 1055–1061.CrossRefGoogle ScholarPubMed
Pothoven, M. A., Beitz, D. C. and Thornton, J. H. 1975. Lipogenesis and lipoiysis in adipose tissue of ad libitum and restricted-fed beef cattle during growth J. Anim. Sci. 40: 957–962.CrossRefGoogle ScholarPubMed
Prescott, J. H. D. and Lamming, G. E. 1964. The effects of castration on meat production in cattle, sheep and pigs. J. agric. Sci., Camb. 63: 341–357.CrossRefGoogle Scholar
Preston, R. L. 1975. Biological responses to estrogen additives in meat producing cattle and lambs. J. Anim. Sci. 41: 1414–1430.CrossRefGoogle Scholar
Prior, R. L. 1978. Effect of level of feed intake on lactate and acetate metabolism and lipogenesis in vivo in sheep. J. Nutr. 108: 926–935.CrossRefGoogle ScholarPubMed
Prior, R. L., Crouse, J. D., Harrison, V. L. and Baile, C. A. 1978. Elfazepam and Synovex-S influences on growth and carcass characteristics of steers fed two dietary energy levels. J. Anim. Sci. 47: 1225–1232.CrossRefGoogle Scholar
Prior, R. L. and Jacobson, J. J. 1979a. Effects of fasting and refeeding and intravenous glucose infusion on in vitro lipogenesis in bovine adipose tissue. J. Nutr. 109: 1279–1284.CrossRefGoogle ScholarPubMed
Prior, R. L. and Jacobson, J. J. 1979b. Lactate, pyruvate and acetate intractions during in vitro lipogenesis in bovine adipose tissue. J. Anim. Sci. 49: 1410–1416.CrossRefGoogle Scholar
Prior, R. L. and Scott, R. A. 1980. Effect of intravenous infusions of glucose, lactate, propionate or acetate on the induction of lipogenesis in bovine adipose tissue. J. Nutr. 110: 2011–2019.CrossRefGoogle ScholarPubMed
Prior, R. L., Smith, S. B. and Jacobson, J. J. 1981. Metabolic pathways involved in lipogenesis from lactate and acetate in bovine adipose tissue: effects of metabolic inhibitors. Archs Biochem. Biophys. 211: 202–210.CrossRefGoogle ScholarPubMed
Schanbacher, B. D., Prior, R. L. and Smith, S. B. 1983. Effects of castration and subdermal silastic implants containing oestradiol-17 β-dipropionate on feedlot performance and carcass characteristics of male cattle. Anim. Prod. 37: 73–80.Google Scholar
Smith, S. B. and Prior, R. L. 1981. Evidence for a functional ATP-citrate lyase: NADP-malic dehydrogenase pathway in ruminant adipose tissue: enzyme and metabolite levels. Archs Biochem. Biophys. 211: 192–201.CrossRefGoogle Scholar
Trenkle, A. H. 1969. The mechanisms of action of estrogens in feeds on mammalian and avian growth. In The Uses of Drugs in Animal Feeds, Nat. Acad. Sci. Publ. No. 1679, p. 150. National Academy of Sciences, Washington, DC.Google Scholar
Turner, H. A., Phillips, R. L., Vavra, M. and Young, D. C. 1981. The efficacy of an estradiolsilicone rubber removeable implant in suckling, growing and finishing steers. J. Anim. Sci. 52: 939–944.CrossRefGoogle Scholar
Umbrett, W. W., Burns, R. H. and Stauffer, J. F. 1957. Manametric Techniques, p. 149. Burgess, Minneapolis.Google Scholar
Utley, P. R., Murphy, C. N., Merchant, C. E. and McCormick, W. C. 1980. Evaluation of estradiol removeable implants for growing and finishing steer calves. J. Anim. Sci. 50: 221–225.CrossRefGoogle Scholar
Whitehurst, G. B., Beitz, D. C., Pothoven, M. H., Ellison, W. R. and Crump, M. H. 1978. Lactate as a precursor of fatty acids in bovine adipose tissue. J. Nutr. 108: 1806–1811.CrossRefGoogle ScholarPubMed
Yang, Y. T. and Baldwin, R. L. 1973. Lipolysis in isolated cow adipose cells. J. Dairy Sci. 56: 366–374.CrossRefGoogle ScholarPubMed