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Injection of neonatal piglets with dexamethasone stimulates leptin mRNA expression and reduces the stress response in market weight pigs

Published online by Cambridge University Press:  18 August 2016

W.J. Meadus ,
R. MacInnis ,
J.L. Aalhus  and
N.J. Cook
W.J. Meadus*
Affiliation:
Meat Research Section, Agriculture and Agri-Food Canada, Lacombe Research Centre, 6000 C&E Trail, Lacombe, Alberta T4L 1W1, Canada
R. MacInnis
Affiliation:
Meat Research Section, Agriculture and Agri-Food Canada, Lacombe Research Centre, 6000 C&E Trail, Lacombe, Alberta T4L 1W1, Canada
J.L. Aalhus
Affiliation:
Meat Research Section, Agriculture and Agri-Food Canada, Lacombe Research Centre, 6000 C&E Trail, Lacombe, Alberta T4L 1W1, Canada
N.J. Cook
Affiliation:
Meat Research Section, Agriculture and Agri-Food Canada, Lacombe Research Centre, 6000 C&E Trail, Lacombe, Alberta T4L 1W1, Canada
*
†E-mail [email protected]
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Abstract

Neonatal piglets were given three daily injections of dexamethasone at 1 mg/kg body weight to examine if their stress response and carcass fat distribution at a 120-kg market weight could be altered. The daily acute injections tended to increase the amount of muscle and adrenal leptin mRNA expression (P < 0·1) for 1 and 3 days after the end of the treatment but had no effect on the amount of glucocorticoid receptor mRNA. The treated animals had a 19% lower adult stress response to transport than the controls as measured by salivary cortisol levels. There was no significant change in the treated animals’ carcass and meat quality traits at market weight. However, a trend towards more marbling fat and carcass backfat were seen in the dexamethasone treated pigs at market weight.

Keywords

cortisoldexamethasoneleptinmeat qualitystress response
Type
Growth, development and meat science
Information
Animal Science , Volume 74 , Issue 1 , February 2002 , pp. 81 - 87
Copyright
Copyright © British Society of Animal Science 2002

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References

American Meat Science Association. 1990. Recommended procedures for beef carcass evaluation and carcass contest, third edition. American Meat Science Association, Chicago, IL.Google Scholar
Brethour, J. R. 1972. Effects of acute injections of dexamethasone on selective deposition of bovine intramuscular fat. Journal of Animal Science 35: 351–356.Google Scholar
Canadian Council on Animal Care. 1993. Guide to the care and use of experimental animals, vol. 1 (ed. Olfert, E.D., Cross, B.M. and McWilliam, A.A.). CCAC, Ottawa, ON.Google Scholar
Chapple, R. P., Cuaron, J. A. and Easter, R. A. 1989. Effect of glucocorticoids and limiting nursing on the carbohydrate digestive capacity and growth rate of piglets. Journal of Animal Science 67: 2956.Google Scholar
Clow, A., Vellucci, S. V., Parrott, R. F., Hucklebridge, F., Sen, S. and Evans, P. 2000. Endogenous monoamine oxidase inhibitory activity and HPA activation in the pig. Life Sciences 66: 35–41.Google Scholar
Cook, N. J., Schaefer, A. L., Lepage, P. and Morgan-Jones, S. 1996. Salivary vs serum cortisol for the assessment of adrenal activity in swine. Canadian Journal of Animal Science 76: 329–335.Google Scholar
Corah, T. J., Tatum, J. D., Morgan, J. B., Mortimer, R. G. and Smith, G. C. 1995. Effects of a dexamethasone implant on deposition of intramuscular fat in genetically identical cattle. Journal of Animal Science 73: 3310–3316.Google Scholar
Dugan, M. E. R., Aalhus, J. L., Jeremiah, L. E., Kramer, J. K. G. and Schaefer, A. L. 1999. The effects of feeding conjugated linoleic acid on subsequent pork quality. Canadian Journal of Animal Science 79: 45–51.CrossRefGoogle Scholar
Dvorak, M. 1972. Adrenocortical function in foetal, neonatal and young pigs. Journal of Endocrinology 54: 473–481.Google Scholar
Eikelenboom, G., Hoving-Bolink, A. H. and Wal, P. G.van der. 1996. The eating quality of pork: the influence of intramuscular fat. Fleischwirschaft 76: 517–518.Google Scholar
Gomez, S., Angeles, M. L. and Cuaron, J. A. 1997. Growth performance and enzyme development in weanling pigs injected with dexamethasone. Journal of Animal Science 75: 993–1000.Google Scholar
Hausman, G. J. and Richardson, R. L. 1998. Newly recruited and pre-existing preadipocytes in cultures of porcine stomal-vascular cells: morphology, expression of extracellular matrix components, and lipid accretion. Journal of Animal Science 76: 48–60.Google Scholar
Hay, M., Meunier-Salaun, M.-C., Brulaud, F., Monnier, M. and Mormede, P. 2000. Assesment of hypothalamic-pituitary-adrenal axis and sympathetic nervous system activity in pregnant sows through the measurement of glucocorticoids and catecholamines in urine. Journal of Animal Science 78: 420–428.Google Scholar
Hollenberg, S. M., Weinberger, C., Ong, E. S., Cerelli, G., Oro, A., Lebo, R., Thompson, E. B., Rosenfeld, M. G. and Evans, R. M. 1985. Primary structure and expression of a functional human glucocorticoid receptor cDNA. Nature 318: 635–641.Google Scholar
Hwang, C.-S., Loftus, T. M., Mandrup, S. and Lane, M. D. 1997. Adipocyte differentiation and leptin expression. Annual Review of Cellular and Developmental Biology 13: 231–259.Google Scholar
Hyun, Y., Ellis, M., Riskowski, G. and Johnson, R. W. 1998. Growth performance of pigs subjected to multiple concurrent environmental stressors. Journal of Animal Science 76: 721–727.Google Scholar
Janss, L. L. G., Arendonk, J. A. M.van and Brascamp, E. W. 1994. Identification of a single gene affecting intramuscular fat in Meishan crossbreds using Gibbs sampling. Proceedings of the fifth world congress on genetics applied to livestock production, Guelph, vol. 18, pp. 361–364.Google Scholar
Jones, S. D. M., Tong, A. K. W., Campbell, C. and Dyck, R. 1994. The effects of fat thickness and degree of marbling on pork colour and structure. Canadian Journal of Animal Science 74: 155–157.Google Scholar
Laferrere, B., Fried, S. K., Osborne, T. and Pi-Sunyer, F.X. 2000. Effect of one morning meal and a bolus of dexamethasone on 24-hour variation of serum leptin levels in humans. Obesity Research 8: 481–486.Google Scholar
McNeel, R. L., Ding, S.-T., O’Brian Smith, E. and Mersmann, H. J. 2000. Effect of feed restriction on adipose tissue transcript concentrations in genetically lean and obese pigs. Journal of Animal Science 78: 934–942.Google Scholar
Malkoski, S. P. and Dorin, R. I. 1999. Composite glucocorticoid regulation at a functionally defined negative glucocorticoid response element of the human corticotropin-releasing hormone gene. Molecular Endocrinology 13: 1629–1644.CrossRefGoogle Scholar
May, S. G., Savell, J. W., Lunt, D. K., Wilson, J. J., Laurenz, J. C. and Smith, S. B. 1994. Evidence for preadipocyte proliferation during culture of subcutaneous and intramuscular adipose tissues from Angus and Wagyu crossbred steers. Journal of Animal Science 72: 3110–3117.Google Scholar
Meany, M. J., Aitken, D. H., Viau, V., Sharma, S. and Sarrieau, A. 1989. Neonatal handling alters adrenocortical negative feedback sensitivity and hippocampal type II glucocorticoid receptor binding in the rat. Neuroendocrinology 50: 597–604.CrossRefGoogle Scholar
Melton, B. E. and Huffman, W. E. 1995. Economics of the NGEP: relating the genetic merit of terminal sire lines to their profit potential. In Genetic evaluation: Terminal Line Program results, pp. 269–308. National Pork Producers Council, Des Moines IA.Google Scholar
Mersmann, H. J. 1990. Metabolic and endocrine control of adipose tissue accretion. In Reducing fat in meat animals (ed. Wood, J. D. and Fisher, A. V.), pp. 101–144. Elsevier Applied Science, London.Google Scholar
Moller, A. J. 1980-81. Analysis of Warner-Bratzler shear pattern with regard to myofibrillar and connective tissue components of tenderness. Meat Science 5: 247–260.Google Scholar
Nakai, Y., Usui, T., Tsukada, T., Takahashi, H., Fukata, J., Fukushima, M., Senoo, K. and Imura, H. 1991. Molecular mechanisms of glucocorticoid inhibition of human proopiomelanocortin gene transcription. Journal of Steroid Biochemistry and Molecular Biology 40: 301–306.Google Scholar
National Research Council. 1988. Nutrient requirements of swine, ninth edition. National Academy Press, Washington, DC.Google Scholar
Oakley, R. H. and Cidowski, J. A. 1993. Homologous down regulation of the glucocorticoid receptor: the molecular machinery. Critical Reviews of Eukaryotic Gene Expression 3: 63–88.Google Scholar
Ohyama, M., Matsuda, K., Torii, S., Matsui, T., Yano, H., Kawada, T. and Ishihara, T. 1998. The interaction between vitamin A and thiozolinedione on bovine adipocyte differentiation in primary culture. Journal of Animal Science 76: 61–65.Google Scholar
Rosmond, R., Chagnon, Y. C., Holm, G., Chagnon, M., Perusse, L., Lindell, K., Carlsson, B., Bouchard, C. and Bjorntorp, P. 2000. A glucocorticoid receptor gene marker is associated with abdominal obesity, leptin, and dysregulation of the hypothalamic-pituitary-adrenal axis. Obesity Research 8: 211–218.Google Scholar
Seckl, J. R., Cleasby, M. and Nyirenda, M. J. 2000. Glucocorticoids, 11b-hydroxysteroid dehydrogenase, and fetal programming. Kidney International 57: 1412–1417.Google Scholar
Shi, X. M., Blair, H. C., Yang, X., McDonald, J. M. and Cao, X. 2000. Tandem repeat of C/EBP binding sites mediates PPARgamma2 gene transcription in glucocorticoid-induced adipocyte differentiation. Journal of Cellular Biochemistry 76: 518–527.Google Scholar
Singleton, J. R., Baker, B. L. and Thorburn, A. 2000. Dexamethasone inhibits insulin-like growth factor signaling and potentiates myoblast apotosis. Endocrinology 141: 2945–2950.Google Scholar
Smith, J. T. and Waddell, B. J. 2000. Increased fetal glucocorticoid exposure delays puberty onset in postnatal life. Endocrinology 141: 2422–2428.Google Scholar
Statistical Analysis Systems Institute. 1999. SAS user’s guide: statistics, eighth edition. SAS Institute Inc., Cary NC.Google Scholar
Student, A. K., Hsu, R. Y. and Lane, M. D. 1980. Induction of fatty acid synthetase synthesis in differentiating 3T3-L1 preadipocytes. Journal of Biological Chemistry 255: 4745–4750.Google Scholar
Tong, A. K. W. and Jones, S. D. M. 1992. National pork carcass cutout project (1992) part 7: verification of quadratic prediction equations for the Hennessy and Destron grading probes to predict carcass value. Lacombe Research Station, Agriculture and Agri-Food Canada.Google Scholar
Trut, L. N. 1999. Early canid domestication: the farm fox experiment. American Scientist 87: 160–169.Google Scholar
Wang, J., Liu, R., Hawkins, M., Barzilai, B. and Rossetti, L. 1998. A nutrient sensing pathway regulates leptin gene expression in muscle and fat. Nature 393: 684–688.Google Scholar
Weaver, S. A., Aherne, F. X., Meaney, M. J., Schaefer, A. L. and Dixon, W. T. 2000. Neonatal handling permanently alters hypothalamic-pituitary-adrenal axis function, behavior, and body weight in boars. Journal of Endocrinology 164: 349–359.Google Scholar
Williams, L. B., Fawcett, R. L., Waechter, A. S., Zhang, P., Kogon, B. E., Jones, R., Inman, M., Huse, J., and Considine, R. V. 2000. Leptin production in adipocytes from morbidly obese subjects: stimulation by dexamethasone, inhibition with troglitazone, and influence of gender. Journal of Clinical Endocrine Metabolism 85: 2678–2684.Google ScholarPubMed