Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-23T22:03:13.649Z Has data issue: false hasContentIssue false
  • English
  • Français

Expression of insulin-like growth factor-1 (IGF-1) and growth hormone-receptor (GHR) mRNA in liver, skeletal muscle and adipose tissue of different breeds of pig

Published online by Cambridge University Press:  02 September 2010

J. M. Brameld ,
J. L. Atkinson ,
T. J. Budd ,
J. C. Saunders ,
J. M. Pell ,
A. M. Salter ,
R. S. Gilmour  and
P. J. Buttery
J. M. Brameld
Affiliation:
University of Nottingham, Department of Applied Biochemistry and Food Science, Sutton Bonington Campus, Loughborough LE12 5RD
J. L. Atkinson
Affiliation:
University of Nottingham, Department of Applied Biochemistry and Food Science, Sutton Bonington Campus, Loughborough LE12 5RD
T. J. Budd
Affiliation:
University of Nottingham, Department of Applied Biochemistry and Food Science, Sutton Bonington Campus, Loughborough LE12 5RD
J. C. Saunders
Affiliation:
Department of Cellular Physiology, The Babraham Institute, Babraham, Cambridge CB2 4AT
J. M. Pell
Affiliation:
Department of Cellular Physiology, The Babraham Institute, Babraham, Cambridge CB2 4AT
A. M. Salter
Affiliation:
University of Nottingham, Department of Applied Biochemistry and Food Science, Sutton Bonington Campus, Loughborough LE12 5RD
R. S. Gilmour
Affiliation:
Department of Cellular Physiology, The Babraham Institute, Babraham, Cambridge CB2 4AT
P. J. Buttery
Affiliation:
University of Nottingham, Department of Applied Biochemistry and Food Science, Sutton Bonington Campus, Loughborough LE12 5RD
Get access

Abstract

The work described was carried out to study the expression of insulin-like growth factor 1 (IGF-1) and growth hormone-receptor (GHR) mRNA in liver, skeletal muscle and adipose tissue from three breeds of pig with varying growth characteristics. The three breeds studied were the Large Wlrite, noted for its lean tissue; the Duroc, characterized by its high intramuscular fat content; and the Meishan × Landrace (0·5 Meishan), noted for its fat, poorly conformed carcass and slower growth rate. The probes used were designed to monitor promoter usage for IGF-1 expression and also expression of the extra-cellular domain of the GHR. Eighteen gilts, six of each breed, were given a barley/wheat diet (158 g crude protein, 10·7 g lysine and 13·9 MJ energy per kg), to appetite, for 1 to 2 weeks until they reached about 85 kg. Samples of liver, longissimu s dorsi (LD) muscle and three adipose tissue depots (subcutaneous (SC), perirenal (PR) and omental (OM)) were collected immediately after slaughter and frozen in liquid nitrogen (total time of sample collection to plunging of sample into liquid nitrogen was <3 min), prior to extraction of total RNA and ribonuclease protection assays. Individual serum samples collected at exsanguination were frozen prior to IGF-1 radioimmunoassay. There were no breed differences in the serum IGF-1 concentrations (range 49 to 134 μg/l), or in expression of the GHR gene or either class of IGF-1 transcript in the liver. However, there was a significant difference between the breeds in expression of IGF-1 mRNA in the LD muscle (P < 0·001), the order being Duroc > Wliite > Meishan, with only class 1 transcripts of IGF-1 being found. GHR expression in LD muscle was lower in White than in the other tivo breeds (P = 0·022). There was a significant difference between the breeds in expression of IGF-1 mRNA (only class 1 transcripts present) in the adipose tissue (P = 0-006), the order being Wliite · Duroc · Meishan, and also a significant depot difference, with expression being highest in the SC depot (P < 0·001). There were no differences between the breeds or depots in expression of GHR mRNA in adipose tissue. The observed differences in muscle and adipose tissue IGF-1 expression may relate to the overall growth of the animal.

Keywords

gene expressiongenotypespigssomatomedinsomatotropin receptor
Type
Research Article
Information
Animal Science , Volume 62 , Issue 3 , June 1996 , pp. 555 - 559
Copyright
Copyright © British Society of Animal Science 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Althen, T. G. and Gerrits, R. J. 1976. Pituitary and serum growth hormone levels in duroc and yorkshire swine genetically selected for high and low backfat. Journal of Animal Science 42:1490–1497.CrossRefGoogle ScholarPubMed
Brameld, J. M., Atkinson, J. L., Saunders, J. C., Pell, J. M., Buttery, P. J. and Gilmour, R. S. 1995b. Effects of growth hormone administration and dietary protein intake on insulin-like growth factor-1 (IGF-1) and growth hormone receptor (GHR) mRNA expression in porcine liver, skeletal muscle and adipose tissue. Journal of Animal Science (in press).CrossRefGoogle Scholar
Brameld, J. M., Buttery, P. J., Weller, P. A. and Gilmour, R. S. 1993. Ontogenic study of expression of IGF-1 and GH-receptor (GHR) mRNA in pig liver and skeletal muscle. Animal Production 56:457 (abstr.).Google Scholar
Brameld, J. M., Weller, P. A., Pell, J. M., Buttery, P. J. and Gilmour, R. S. 1995a. Ontogenic study of insulin-like growth factor-1 and growth hormone receptor mRNA expression in porcine liver and skeletal muscle. Animal Science 61:333–339.CrossRefGoogle Scholar
Buonomo, F. C. and Klindt, J. 1993. Ontogeny of growth hormone (GH), insulin-like growth factors (IGF-1 and IGF-2) and IGF binding protein-2 (IGFBP-2) in genetically lean and obese swine. Domestic Animal Endocrinology 4:257–265.CrossRefGoogle Scholar
Buonomo, F. C., Lauterio, T. J., Baile, C. A. and Campion, D. R. 1987. Determination of insulin-like growth factor-1 (IGF1) and IGF binding protein levels in swine. Domestic Animal Endocrinology 4:23–31.CrossRefGoogle ScholarPubMed
Chomczynski, P. and Sacchi, N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–169.CrossRefGoogle ScholarPubMed
Coleman, M. E., Russell, L. and Etherton, T. D. 1994. Porcine somatotropin (pST) increases IGF-1 mRNA abundance in liver and subcutaneous adipose tissue but not in skeletal muscle of growing pigs. Journal of Animal Science 72:918–924.CrossRefGoogle Scholar
Haley, C. S., d'Agaro, E. and Ellis, M. 1992. Genetic components of growth and ultrasonic fat depth traits in meishan and Large White pigs and their reciprocal crosses. Animal Production 54:105–115.Google Scholar
Kasser, T. R., Martin, R. J., Gahagan, J. H. and Wangsness, P. J. 1981. Fasting plasma hormones and metabolites in feral and domestic new born pigs. Journal of Animal Science 53:420–426.CrossRefGoogle Scholar
Krick, B. J., Roneker, K. R., Boyd, R. D., Beermann, D. H., David, P. J. and Meisinger, D. J. 1992. Influence of genotype and sex on the response of growing pigs to recombinant porcine somatotropin. Journal of Animal Science 70:3024–3034.CrossRefGoogle ScholarPubMed
Kyriazakis, I., Dotas, D. and Emmans, G. C. 1994. The effect of breed on the relationship between feed composition and the efficiency of protein utilization in pigs. British Journal of Nutrition 71:849–859.CrossRefGoogle ScholarPubMed
Kyriazakis, I., Leus, K., Emmans, G. C., Haley, C. S. and Oldham, J. D. 1993. The effect of breed (Large White × Landrace v. purebred Meishan) on the diets selected by pigs given a choice between two foods that differ in their crude protein contents. Animal Production 56:121–128.Google Scholar
Lauteric, T. J., Trivedi, B., Kapadia, M. and Daughaday, W. H. 1988. Reduced 125-1-hGH binding by serum of dwarf pigs but not by serum of dwarfed poodles. Comparative Biochemistry and Physiology 91A:15–19.CrossRefGoogle Scholar
Lee, C. Y., Chung, C. S. and Simmen, F. A. 1993. Ontogeny of the porcine insulin-like growth factor system. Molecular and Cellular Endocrinology 93:71–80.CrossRefGoogle ScholarPubMed
Louveau, I., Bonneau, M. and Salter, D. N. 1991. Age-related changes in plasma porcine growth hormone (GH) profiles and insulin-like growth factor-1 (IGF-1) concentrations in Large White and Meishan pigs. Reproduction Nutrition Development 31:205–216.CrossRefGoogle ScholarPubMed
Martin, R. J., Ramsay, T. G., Campion, D. R. and Hausman, G. J. 1985. Fetal hormone and metabolite levels in lean and obese pigs. Growth 49:400–407.Google ScholarPubMed
Möller, C., Arner, P., Sonnenfeld, T. and Norstedt, G. 1991. Quantitative comparison of insulin-like growth factor mRNA levels in human and rat tissues analysed by a solution hybridization assay. Journal of Molecular Endocrinology 7:213–222.CrossRefGoogle ScholarPubMed
Pell, J. M., Saunders, J. C. and Gilmour, R. S. 1993. Differential regulation of transcription initiation from insulin-like growth factor-1 (IGF-1) leader exons and of tissue IGF-1 expression in response to changed growth hormone and nutritional status in sheep. Endocrinology 132:1797–1807.CrossRefGoogle ScholarPubMed
Saunders, J. C., Dickson, M. C., Pell, J. M. and Gilmour, R. S. 1991. Expression of a growth hormone-responsive exon of the ovine insulin-like growth factor-I gene. Journal of Molecular Endocrinology 7:233–240.CrossRefGoogle ScholarPubMed
Serra, J. J., Ellis, M. and Haley, C. S. 1992. Genetic components of carcass and meat quality traits in Meishan and Large White pigs and their reciprocal crosses. Animal Production 54:117–127.Google Scholar
Wangsness, P. J., Martin, R. J. and Gahagan, J. H. 1977. Insulin and growth hormone in lean and obese pigs. American Journal of Physiology 233:E104–E108.Google ScholarPubMed
Weller, P. A., Dauncey, M. J., Bates, P. C., Brameld, J. M., Buttery, P. J. and Gilmour, R. S. 1994. Regulation of porcine insulin-like growth factor 1 and growth hormone receptor mRNA expression by energy status. American Journal of Physiology 266:E776–E785.Google ScholarPubMed
Weller, P. A., Dickson, M. C., Huskisson, N. S., Dauncey, M. J., Buttery, P. J. and Gilmour, R. S. 1993. The porcine insulin-like growth factor-1 gene: characterization and expression of alternate transcription sites. Journal of Molecular Endocrinology 11:201–211.CrossRefGoogle ScholarPubMed
White, B. R., Lan, Y. -H., McKeith, F. K., McLaren, D. G., Novakofski, J., Wheeler, M. B. and Kasser, T. R. 1993. Effects of porcine somatotropin on growth and carcass composition of meishan and yorkshire barrows. Journal of Animal Science 71:3226–3238.CrossRefGoogle ScholarPubMed
Wolverton, C. K., Azain, M. J., Duffy, J. Y., White, M. E. and Ramsay, T. G. 1992. Influence of somatotropin on lipid metabolism and IGF gene expression in porcine adipose tissue. American Journal of Physiology 263:E637–E645.Google ScholarPubMed