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Estimation of roughage intake in sheep using a known daily intake of a labelled supplement

Published online by Cambridge University Press:  09 March 2007

C. Elwert*
Affiliation:
Institut für Ernährungswissenschaften, Martin-Luther-Universität Halle-Wittenberg, 06099 Halle, Germany
H. Dove
Affiliation:
CSIRO Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia
*
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Abstract

A feeding trial with growing sheep was conducted on mixed roughage-supplement diets, to test whether roughage intake could be estimated from a known intake of a supplement and an alkane-based estimate of the supplement and roughage proportions in the diet (diet composition) without the need for separate dosing with external marker alkanes. Diets consisted of chaffed Trifolium subterraneum hay (SC), or a mix of SC and beeswax-labelled cottonseed meal (CSM) in the proportions of 7: 1, 6: 2, 5: 3 and 4: 4 (air-dry matter basis). Six sheep were given the pure SC diet and three sheep were given each of the mixed diets. Additionally, the CSM was also labelled with octatriacontane (C38) to investigate its use for labelling supplements. Due to unexpectedly low recoveries, C38 was not included in any estimation of diet composition or digestibility.

The inclusion of beeswax-labelled CSM in a SC diet resulted in lower faecal alkane recoveries (as proportions of alkane ingested; P ≤ 0·05). Within mixed diets, recoveries decreased significantly with increasing proportion of CSM, but this effect was significant (P < 0·05) only for alkanes consisting of 25, 26, 27 and 29 carbon-atoms. Estimates of diet composition were close to measured values. Daily SC intake and organic matter digestibility (OMD) differed by −3·7 to 7·2% and −2·0 to 5·7% from measured values if a mean faecal recovery, across diets, was applied for each alkane. Accuracy increased greatly if estimates were based upon faecal alkane recoveries for individual diets, instead of mean recoveries across diets (−0·3 to 0·9% and −0·2 to 0·0% for SC intake and OMD, respectively).

The results indicate that roughage intake can be estimated from a combination of known supplement intake and an estimate of diet composition, which obviates the need for separate alkane dosing to estimate intake. The method should be applicable in situations in which animals either normally receive supplements (e.g., dairy cows) or could readily be given supplements.

Type
Research Article
Copyright
Copyright © British Society of Animal Science 2005

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References

Bibby, J. and Toutenburg, H. 1977. Prediction and improved estimation in linear models. John Wiley and Sons, Chichester.Google Scholar
Brosh, A., Henkin, Z., Rothman, S. J., Aharoni, Y., Orlov, A. and Arieli, A. 2003. Effects of faecal n-alkane recovery in estimates of diet composition. Journal of Agricultural Science, Cambridge 140: 93100.CrossRefGoogle Scholar
Dillon, P. G. 1993. The use of n-alkanes as markers to determine herbage intake, botanical composition of available or consumed herbage and in studies of digesta kinetics with dairy cows. Ph. D. thesis, National University of Ireland, Dublin.Google Scholar
Dove, H. and Coombe, J. B. 1992. A comparison of methods for estimating supplement intake and diet digestibility in sheep. Animal Production in Australia 19: 239241.Google Scholar
Dove, H. and Mayes, R. W. 1991. The use of plant wax alkanes as marker substances in studies of the nutrition of herbivores: a review. Australian Journal of Agricultural Research 42: 913952.CrossRefGoogle Scholar
Dove, H. and Mayes, R. W. 1996. Plant wax components: a new approach to estimating intake and diet composition in herbivores. Journal of Nutrition 126: 1326.CrossRefGoogle ScholarPubMed
Dove, H. and Mayes, R. W. 1999. Developments in the use of plant wax markers for estimating diet selection in herbivores. In Emerging techniques for studying the nutrition of free ranging herbivores (ed. Dove, H. and Coleman, S. W.), satellite meeting of the fifth international symposium on the nutrition of herbivores, San Antonio, Texas (CD-ROM).Google Scholar
Dove, H., Mayes, R. W. and Freer, M. 1995. Using cuticular wax alkanes to estimate herbage intake in animals fed supplements. Annales de Zootechnie 44: (suppl.) 237.CrossRefGoogle Scholar
Dove, H., Mayes, R. W., Lamb, C. S. and Ellis, K. J. 2002a. Factors influencing the release rate of alkanes from an intra-ruminal, controlled-release device, and the resultant accuracy of intake estimation in sheep. Australian Journal of Agricultural Research 53: 681696.CrossRefGoogle Scholar
Dove, H. and Moore, A. D. 1995. Using a least-squares optimization procedure to estimate botanical composition based on the alkanes of plant cuticular wax. Australian Journal of Agricultural Research 46: 15351544.CrossRefGoogle Scholar
Dove, H. and Oliván, M. 1998. Using synthetic or beeswax alkanes for estimating supplement intake in sheep. Animal Production in Australia 22: 189192.Google Scholar
Dove, H., Scharch, C., Oliván, M. and Mayes, R. W. 2002b. Using n-alkanes and known supplement intake to estimate roughage intake in sheep. Animal Production in Australia 24: 5760.Google Scholar
Dove, H., Wood, J. T., Simpson, R. J., Leury, B. J., Ciavarella, T. A., Gatford, K. L. and Siever-Kelly, C. 1999. Spray-topping annual grass pasture with glyphosate to delay loss of feeding value during summer. III. Quantitative basis of the alkane-based procedures for estimating diet selection and herbage intake by grazing sheep. Australian Journal of Agricultural Research 50: 475485.CrossRefGoogle Scholar
Dufner, J., Jensen, U. and Schumacher, E. 2002. Statistik mit SAS. B. G. Teubner, StuttgartCrossRefGoogle Scholar
Elwert, C. 2004. Studies on the use of alkanes to estimate diet composition, intake, and digestibility in sheep. Ph. D. thesis, Martin-Luther Universität Halle-Wittenberg, Halle.Google Scholar
Elwert, C., Kluth, H. and Rodehutscord, M. 2004. Effect of variable intake of alfalfa and wheat on faecal alkane recoveries and estimates of roughage intake in sheep. Journal of Agricultural Science, Cambridge 142: 213223.CrossRefGoogle Scholar
Elwert, C. and Rodehutscord, M. 2004. Notes on the use of beeswax as a source of alkanes for the alkane technique. Grassland Science in Europe 9: 10521054.Google Scholar
Jeffree, C. E. 1986. The cuticle, epicuticular waxes and trichomes of plants, with reference to their structure, functions and evolution. In Insects and the plant surface (ed. Juniper, B and Southwood, T. R. E.), pp. 2364. Edward Arnold, London.Google Scholar
Jetter, R., Schaeffer, S. and Riederer, M. 2000. Leaf cuticular waxes are arranged in chemically and mechanically distinct layers: evidence from Prunus laurocerasus L. Plant Cell and Environment 23: 619628.CrossRefGoogle Scholar
Mayes, R. W., Beresford, N. A., Lamb, C. S., Barnett, C. L., Howard, B. J., Jones, B. -E., Eriksson, O., Hove, K., Pedersen, Ø. and Staines, B. W. 1994. Novel approaches to the estimation of intake and bioavailability of radiocaesium in ruminants grazing forested areas. The Science of the Total Environment 157: 289300.CrossRefGoogle Scholar
Mayes, R. W. and Duncan, A. J. 1999. New developments in the use of plant-wax markers to determine intake. In Emerging techniques for studying the nutrition of free ranging herbivores (ed. Dove, H. and Coleman, S. W.), satellite meeting of the fifth international symposium on the nutrition of herbivores, San Antonio, Texas (CD-ROM).Google Scholar
Mayes, R. W., Lamb, C. S. and Colgrove, P. M. 1986. The use of dosed and herbage n-alkanes as markers for the determination of herbage intake. Journal of Agricultural Science, Cambridge 107: 161170.CrossRefGoogle Scholar
Molina, D. O., Matamoros, I. and Pell, A. N. 2004. Accuracy of estimates of herbage intake of lactating cows using alkanes: comparison of two types of capsules. Animal Feed Science and Technology 114: 241260.CrossRefGoogle Scholar
Newman, J. A., Cribari-Neto, F. and Jensen, M. J. 1998. The sensitivity of n-alkane analysis to measurement error: implications for use in the study of diet composition. Journal of Agricultural Science, Cambridge 131: 465476.CrossRefGoogle Scholar
Oliván, M., Dove, H., Mayes, R. W. and Hoebee, S. E. 1999. Recent developments in the use of alkanes and other plant wax components to estimate intake and diet composition in herbivores. Revista Portuguesa de Zootecnia 6: 126.Google Scholar
Oliván, M. and Osoro, K. 1995. The use of n-alkanes for estimating feed intake in beef cows. Annales de Zootechnie 44: (suppl.) 239.CrossRefGoogle Scholar
Oliván, M. and Osoro, K. 1999. Effect of temperature on alkane extraction from faeces and herbage. Journal of Agricultural Science, Cambridge 132: 305312.CrossRefGoogle Scholar
Statistical Analysis Systems Institute. 2001. SAS version 8·2. SAS Institute Inc., Cary, NC.Google Scholar
Valiente, O. L., Delgado, P., de Vega, A. and Guada, J. A. 2003. Validation of the n-alkane technique to estimate intake, digestibility, and diet composition in sheep consuming mixed grain:roughage diets. Australian Journal of Agricultural Research 54: 693702.CrossRefGoogle Scholar