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Enhanced pyridoxine supplementation of diets for gilthead seabream (Sparus aurata L.)

Published online by Cambridge University Press:  02 September 2010

P. C. Morris
Affiliation:
Fish Nutrition Unit, Department of Biological Sciences, University of Plymouth, Drake Circus, Plymouth, Devon PL4 8AA
S. J. Davies
Affiliation:
Fish Nutrition Unit, Department of Biological Sciences, University of Plymouth, Drake Circus, Plymouth, Devon PL4 8AA
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Abstract

Using a high protein diet (483 g/kg), two trials were carried out to assess any potential benefit which may result from the supplementation of diets for fingerling and grower class gilthead seabream with pyridoxine at levels which were below, matched and far exceeded the minimum dietary requirement. At the level of supplementation below the minimal requirement (lowest level) the responses from the practical diet almost matched the responses from the diet containing an amount of pyridoxine corresponding to the published minimum requirement for the species and no significant improvement in performance was recorded in response to increasing supplement level. However, despite the absence of a marked effect on overall performance, a small potential for increased activity of alanine aminotransferase was recorded amongst grower class fish given diets containing the lowest supplement. The proximate composition of the grower class fish was unaffected by the level of pyridoxine supplementation though marginal increases in the lipid content of the fingerlings were observed. At the haematological level, haematocrit, total haemoglobin and the plasma concentrations of glucose and protein were also unaffected. However, on the application of an acute stressor (repeated netting), significant alterations in haematocrit and plasma glucose concentration reflecting dietary pyridoxine supplement were recorded.

Type
Research Article
Copyright
Copyright © British Society of Animal Science 1995

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References

Arnadottir, M., Brattström, L., Simonsen, O., Thysell, H., Hultberg, B., Andersson, A. and Nilsson-Ehle, , P. 1993. The effect of high-dose pyridoxine and folic acid supplementation on serum lipid and plasma homocysteine concentrations in dialysis patients. Clinical Nephrology 40: 236240.Google ScholarPubMed
Association of Official Analytical Chemists. 1990. Official methods of analysis, 15th ed. (ed. Herlich, K.). Association of Official Analytical Chemists, Arlington, Virginia.Google Scholar
Avella, M., Young, G., Prunet, P. and Schreck, C. B. 1990. Plasma prolactin and cortisol concentrations during salinity challenges of coho salmon (Oncorhynchus kisutch) at smolt and post-smolt stages. Aquaculture 91: 359372.CrossRefGoogle Scholar
Baker, R. T. M. and Davies, S. J. 1995. The effect of pyridoxine supplementation on dietary protein utilisation in gilthead seabream fry. Animal Science 60: 157162.CrossRefGoogle Scholar
Barnes, H. and Blackstock, J. 1973. Estimation of lipids in marine animals and tissues: detailed investigation of the sulphophosphovanillin method for ‘total’ lipids. Journal of Experimental Marine Biology and Ecology 12: 103118.CrossRefGoogle Scholar
Bender, D. A. 1992. Nutritional biochemistry of vitamins. Cambridge Univeristy Press.Google Scholar
Brooksbank, M. A. 1993. Tissue activity profile of alanine aminotransferase, with respect to the level of vitamin B6 in diets for the gilthead seabream (Sparus aurata). M.Sc. thesis, University of Plymouth.Google Scholar
Chandra, R. and Sudhakaran, L. 1990. Regulation of immune responses by vitamin B6. Annals of the New York Academy of Sciences 585: 404423.CrossRefGoogle ScholarPubMed
Cohen, M. and Bendich, A. 1986. Safety of pyridoxine — a review of human and animal studies. Toxicology Letters 34: 129139.CrossRefGoogle ScholarPubMed
Cowey, C. B. and Sargent, J. R. 1979. Nutrition. In Fish physiology, vol. VIII, pp. 10169. Academic Press, London.Google Scholar
Cowey, C. B. and Walton, M. J. 1989. Intermediary metabolism. In Fish nutrition. 2nd ed. (ed. Halver, J. E.), pp. 260321. Academic Press, London.Google Scholar
Davis, K. B. and Parker, N. C. 1983. Plasma corticosteroid and chloride dynamics in rainbow trout, Atlantic salmon and lake trout during and after stress. Aquaculture 32: 189194.CrossRefGoogle Scholar
Duncan, D. 1955. Multiple range tests and multiple F tests. Biometrics 11: 142.CrossRefGoogle Scholar
Fletcher, G. L. 1975. Effects of capture stress and storage of whole blood on the red cells, plasma proteins, glucose and electrolytes of winter flounder, Pseudopleuronectes americanus. Canadian journal of Zoology 53: 197206.CrossRefGoogle ScholarPubMed
Flos, R., Reig, L., Torres, P. and Tort, L. 1988. Primary and secondary stress responses to grading and hauling in rainbow trout, Salmo gairdneri. Aquaculture 71: 99106.CrossRefGoogle Scholar
Foo, J. T. W. and Lam, T. J. 1993. Serum cortisol response to handling stress and the effect of cortisol implantation on testosterone level in the tilapia, Oreochromis mossambicus. Aquaculture 115: 145158.CrossRefGoogle Scholar
Halver, J. E. 1989. Vitamins. In Fish nutrition. 2nd ed. (ed. Halver, J. E.), pp. 32102. Academic Press, London.Google Scholar
Hardy, R. W., Halver, J. E. and Brannon, E. L. 1979. Effect of dietary protein on the pyridoxine requirement and disease resistance of chinook salmon. In Finfish nutrition and fishfeed technology, vol. 1 (ed. Halver, J. E. and Tiews, K.), proceedings of the world symposium on finfish nutrition and fishfeed technology, Hamburg, June 1978, pp. 253260.Google Scholar
Hunn, J. B. and Greer, I. E. 1991. Influence of sampling on the blood chemistry of Atlantic salmon. Progressive Fish-Culturist 53: 184187.2.3.CO;2>CrossRefGoogle Scholar
Hopkins, T. E. and Cech, J. J. 1992. Physiological effects of capturing striped bass in gill nets and fyke traps. Transactions of the American Fisheries Society 121: 819822.2.3.CO;2>CrossRefGoogle Scholar
Kissil, G. Wim., Cowey, C. B., Adron, J. W. and Richards, R. H. 1981. Pyridoxine requirements of the gilthead seabream, Sparus aurata. Aquaculture 23: 243245.CrossRefGoogle Scholar
Lakshimi, R., Lakshimi, A. V., Divan, P. V. and Bamji, M. S. 1991. Effect of riboflavin or pyridoxine deficiency on inflammatory response. Indian Journal of Biochemistry and Biophysics 28: 481484.Google Scholar
Leklem, J. E. 1991. Vitamin B6 In Handbook of vitamins (ed. Machlin, L. J.), pp. 341392. Dekker, N.Y.Google Scholar
McDonald, D. G., Goldstein, M. D. and Mitton, C. 1993. Responses of hatchery-reared brook trout, lake trout and splake to transport stress. Transactions of the American Fisheries Society 122: 11271138.2.3.CO;2>CrossRefGoogle Scholar
Morris, P. C., Davies, S. J. and Lowe, D. M. 1995. Qualitative requirement for B vitamins in diets for the gilthead seabream (Sparus aurata L.). Animal Science 61: 419426.CrossRefGoogle Scholar
Palm, D., Klein, H. W., Schinzel, R., Buehner, M. and Helmreich, E. J. M. 1990. The role of pyridoxal 5'-phosphate in glycogen phosphorylase catalysis. Biochemistry 29: 10991106.CrossRefGoogle ScholarPubMed
Selvam, R. and Ravichandran, V. 1991. Lipid peroxidation in liver of vitamin B-6 deficient rats, journal of Nutritional Biochemistry 2: 245250.CrossRefGoogle Scholar
Shearer, K. D. 1994. Factors affecting the proximate composition of cultured fishes with emphasis on salmonids. Aquaculture 119: 6388.CrossRefGoogle Scholar
Soengas, J. L., Rey, P., Rozas, G., Andres, M. D. and Aldegunde, M. 1992. Effects of cortisol and thyroid hormone treatment on the glycogen metabolism of selected tissues of domesticated rainbow trout, Oncorhynchus mykiss. Aquaculture 101: 317328.CrossRefGoogle Scholar
Steffens, W. 1989. Principles offish nutrition. Ellis Harwood, Chichester.Google Scholar
Tacon, A. G. J. 1991. Vitamin nutrition in shrimp and fish. Proceedings of the aquaculture and feed processing workshop, Thailand and Indonesia (ed. Akiyama, D. M. and Tan, R.K.H.), pp. 1041.Google Scholar
Tacon, A. G. J. and Cowey, C. B. 1985. Protein and amino acid requirements. In Fish energetics — new perspectives (ed. Tytler, P. and Calow, P.), pp. 155183. Croom Helm, London.CrossRefGoogle Scholar
Thomas, P. and Robertson, L. 1991. Plasma cortisol and glucose stress responses of red drum (Sciaenops ocellatus) to handling and shallow water stressors and anaesthesia with MS-222, quinaldene sulfate and metomidate. Aquaculture 96: 6986.CrossRefGoogle Scholar
Wedemeyer, G. A. and Yasutake, W. T. 1977. Clinical methods for the assessment of the effects of environmental stress on fish health. Technical papers of the US Fish and Wildlife Service, no. 89. US Department of the Interior, Fish and Wildlife Service, Washington DC.Google Scholar
Wilson, R. P. 1989. Amino acids and proteins. In Fish nutrition (ed. Halver, J. E.), pp. 111151. Academic Press, London.Google Scholar