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Endogenous ileal nitrogen and amino acid flows in the growing pig receiving a protein-free diet and diets containing enzymically hydrolysed casein or graded levels of meat and bone meal

Published online by Cambridge University Press:  18 August 2016

A. Donkoh  and
P. J. Moughan
A. Donkoh*
Affiliation:
Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand
P. J. Moughan
Affiliation:
Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand
*
† Present address: Animal Science Department, University of Science and Technology, Kumasi, Ghana.
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Abstract

Endogenous ileal amino acid flows were determined in pigs fitted with simple T-cannulas using either the regression method (meat and bone meal (MBM) as the protein source at five levels of inclusion) or following protein-free alimentation. Amino acid flows were compared with those determined by feeding animals a diet the sole nitrogen source of which was enzyme-hydrolysed casein (EHC), followed by centrifugation and ultrafiltration of the ileal digesta. The EHC was a mixture of free amino acids and oligopeptides (molecular weight 5000 Da). For the EHC treatment, the ileal digesta precipitate plus retentate was used to determine the endogenous flows. The ultrafiltration step excludes unabsorbed dietary amino acids from the measure of endogenous loss. Chromium III oxide was the reference marker in all diets. Estimates of endogenous nitrogen and amino acid flows determined under protein-free alimentation and the comparable flows determined using the regression method were similar. However, endogenous flows of amino acids for the EHC-fed pigs were generally significantly higher (F < 0·01) than values found for pigs on the protein-free diet and were higher than values obtained after extrapolation for pigs given the MBM-based diets. Mean endogenous ileal nitrogen flow for the EHC-fed animals was 2526 (s.e. 33.9) compared with 1711 (s.e. 25.5) mg/kg dry-matter intake for pigs receiving the protein-free diet.

Keywords

amino acidscaseinileumnitrogenpigs
Type
Research Article
Information
Animal Science , Volume 68 , Issue 3 , April 1999 , pp. 511 - 518
Copyright
Copyright © British Society of Animal Science 1999

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