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Effects of post-ruminal fermentation on the faecal and urinary excretion of purines

Published online by Cambridge University Press:  02 September 2010

J. C. Surra ,
J. A. Guada ,
J. Balcells  and
C. Castrillo
J. C. Surra
Affiliation:
Departamento de Production Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain
J. A. Guada
Affiliation:
Departamento de Production Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain
J. Balcells
Affiliation:
Departamento de Production Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain
C. Castrillo
Affiliation:
Departamento de Production Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain
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Abstract

The effect of post-ruminal fermentation on faecal output of purine bases (PB) and urinary excretion of their derivatives (PD) was studied by the infusion of yeast RNA (2·9 g/day), corn starch (100 g/day), cellulose (200 g/day) and saline solutions into the gastro-intestinal tract offour sheep (35·4 kg live weight) fitted with caecal and duodenal catheters and given 0-80 kg/day alfalfa hay. All substrates were infused into the caecum except cellulose that was infused into the proximal duodenum.

The infusion of RNA did not affect either the faecal excretion of PB or the urinary excretion of allantoin or total PD, although xanthine excretion increased significantly from <0·01 to 0·12 mmol/day.

Starch and cellulose infusions promoted a significant increase in the faecal excretion of diaminopimelic acid (206 and 159 v. 131 mg/day) and PB (4·55 and 3·62 v. 2·29 mmol/day) and modified the partitioning of total nitrogen losses between faeces and urine. The urinary excretion of allantoin and total PD were not affected by the caecal infusion of starch (6·60 and 22·9 v. 7·02 and 22·8 mmol/day) but both tended to increase with the duodenal infusion of cellulose (7·75 and 26·4 mmol/day).

It is concluded that the urinary excretion ofPD is independent of either the supply of nucleic acids to the caecum or the extent of hind gut fermentation although it may be affected by variations in the flow of undigested fibre along the small intestine.

Keywords

caecum fermentationpurinessheep
Type
Research Article
Information
Animal Science , Volume 65 , Issue 3 , December 1997 , pp. 383 - 390
Copyright
Copyright © British Society of Animal Science 1997

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References

Association of Official Analytical Chemists. 1980. Official methods of analysis of the Association of Official Chemists, 13th edition. Association of Official Analytical Chemists, Washington, DC.Google Scholar
Balcells, J., Guada, J. A., Castrillo, C. and Gasa, J. 1991. Urinary excretion of allantoin precursors by sheep after different rates of purine infusion into the duodenum. Journal of Agricultural Science, Cambridge 116: 309–317.CrossRefGoogle Scholar
Balcells, J., Guada, J. A., Peiró, J. M. and Parker, D. S. 1992. Simultaneous determination of allantoin and oxipurines in biological fluids by high-performance liquid chromatography. journal of Chromatography 575:153–157.CrossRefGoogle Scholar
Beckers, Y., Théwis, A., Sohy, C. and François, E. 1995. Measurement of microbial N flow to the duodenum and urinary excretion of purine derivatives in bulls. IVth international symposium on the nutrition of herbivores. Annales de Zootechnie 44:(suppl. 1) 177.CrossRefGoogle Scholar
Bergner, H., Bartelt, J., Kijora, C. and Götz, K. P. 1991. [Demonstration of microbial protein synthesis in the small intestine of sheep with an intraduodenal infusion of 15N-urea.] Archives of Animal Nutrition 41: 765–768.Google Scholar
Bergner, H., Simon, O. and Zimmer, M. 1975. [Content of crude fibre in the diet as affecting the process of amino acid resorption in rats.] Archivfur Tierernahrung 25: 95–104.CrossRefGoogle Scholar
Berlin, R. D. and Hawkins, R. A. 1968. Secretion of purines by the small intestine: general characteristics. American Journal ofPhysiology 215: 932–941.CrossRefGoogle ScholarPubMed
Bruchem, J. van, Bongers, L. J. G. M., Lammers-Wienhoven, S. C. W., Bangma, G. A. and Adrichem, P. W. M. van. 1989. Apparent and true digestibility of protein and amino acids in the small intestine of sheep as related to the duodenal passage of protein and non-protein dry matter. Livestock Production Science 23: 317–327.CrossRefGoogle Scholar
Bruchem, J. van, Bosch, M., Bongers, L. J. G. M., Bangma, G. A. and Adrichem, P. W. M. van. 1987. The direct determination of the endogenous protein content of the digesta leaving the abomasum of the ruminant. In Protein metabolism and nutrition. European Association of Animal Production publication no. 35. EAAP, Rostock.Google Scholar
Chen, X. B. 1989. Excretion of purine derivatives by sheep and cattle and its use for the estimation of absorbed microbial protein. Ph.D. thesis, University of Aberdeen.Google Scholar
Chen, X. B., Hovell, F. D. DeB. and Ørskov, E. R. 1990a. Excretion of purine derivatives by ruminants: recycling of allantoin into the rumen via saliva and its fate in the gut. British Journal of Nutrition 63:197–205.CrossRefGoogle ScholarPubMed
Chen, X. B., Hovell, F. D. DeB., Ørskov, E. R. and Brown, D. S. 1990b. Excretion of purine derivatives by ruminants: effect of exogenous nucleic acid supply on purine derivative excretion by sheep. British Journal of Nutrition 63: 131–142.CrossRefGoogle ScholarPubMed
Chen, X. B., Kyle, D. J., Ørskov, E. R. and Hovell, F. D. DeB. 1991. Renal clearance of plasma allantoin in sheep. Experimental Physiology 76: 59–65.CrossRefGoogle ScholarPubMed
Clark, J. H., Klusmeyer, T. H. and Cameron, M. R. 1992. Microbial protein synthesis and flows of nitrogen fractions to the duodenum of dairy cows. Journal of Dairy Science 75: 2304–2323.CrossRefGoogle Scholar
Czerkawski, J. W. and Faulds, C. 1974. Methods for determining 2-6-diamonopimelic acid an d 2-aminoethylphosphonic acid in gut contents. Journal of the Science of Food and Agriculture 25: 45–55.CrossRefGoogle Scholar
Egan, A. R., Boda, K. and Varaday, J. 1986. Regulation of nitrogen metabolism and recycling. In Control of digestion and metabolism in ruminants (ed. Milligan, L. P., Grovum, W. L. and Dobson, A.), pp. 386–402. Prentice-Hall Publishers, Englewoods Cliffs, NJ.Google Scholar
El-Shazly, K. and Hungate, R. E. 1966. Method for measuring diaminopimelic acid in total rumen contents and its application to the estimation of bacterial growth. Applied Microbiology 14: 27–30.CrossRefGoogle Scholar
Giraldez, F. J., Pelaez, R. and Zorita, E. 1991. [The influence of dietary degradable protein and metabolizable energy ratio on organic matter digestibility and endogenous nitrogen losses.] ITEA, extra 11:187–189.Google Scholar
Goering, H. K. and Van Soest, P. J. 1970. Forage fiber analysis. Agriculture Handbook no. 379 Agricultural Research Service, US Department of Agriculture, Washington DC.Google Scholar
Hoover, W. H. 1978. Digestion and absorption in the hindgut of ruminants. journal of Animal Science 46:1789–1799.CrossRefGoogle ScholarPubMed
Hvelplund, T. and Madsen, J. 1985. Amino acid passage to the small intestine in dairy cows compared with estimates of microbial protein and undegraded dietary protein from analysis on the feed. Ada Agriculturae Scandinavica, Supplement 25: 21–36.Google Scholar
Lange, C. F. M. de, Sauer, W. C., Mosenthin, R. and Souffrant, W. B. 1989. The effect of feeding different protein-free diets on the recovery and amino acid composition of endogenous protein collected from the distal ileum and feces in pigs. Journal of Animal Science 67:746–754.CrossRefGoogle ScholarPubMed
McAllan, A. B. and Smith, R. H. 1973a. Degradation of nucleic acids in the rumen. British Journal of Nutrition 29:331–345.CrossRefGoogle ScholarPubMed
McAllan, A. B. and Smith, R. H. 1973b. Degradation of nucleic acid derivatives by rumen bacteria in vitro. British Journal of Nutrition 29: 467–474.CrossRefGoogle ScholarPubMed
Mason, V. C. 1969. Some observations on the distribution and origin of nitrogen. Journal of Agricultural Science, Cambridge 73:99–111.CrossRefGoogle Scholar
Mason, V. C. and White, F. 1971. The digestion of bacterial mucopeptide constituents in the sheep. 1. The metabolism of 2,6-diaminopimelic acid. Journal of Agricultural Cambridge 77: 91–98.CrossRefGoogle Scholar
Menke, K. H., Raab, L., Salewski, A., Steingass, H., Fritz, D. and Schneider, W. 1979. The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with the rumen liquor in vitro. Journal Agricultural Science, Cambridge 93:217–222.CrossRefGoogle Scholar
Nicoletti, J. M., Davis, C. L., Hespell, R. B. and Leedle, J. A. Z. 1983. Enumeration and presumptive identification of bacteria from the small intestine of sheep. Journal of Dairy Science 67:1227–1235.CrossRefGoogle Scholar
Ørskov, E. R., Fraser, C., Mason, V. C. and Mann, S. O. 1970. Influence of starch digestion in the intestine of sheep on caecal fermentation, caecal microflora and faecal nitrogen excretion. British Journal of Nutrition 24: 671–682.CrossRefGoogle ScholarPubMed
Razzaque, M. A., Topps, J. M. and Kay, R. N. 1981. Metabolism of the nucleic acids of rumen bacteria by preruminant and ruminant lambs. British Journal of Nutrition 45: 517–527.CrossRefGoogle ScholarPubMed
Schneeman, B. O., Richter, D. B. and Jacobs, L. R. 1982. Response to dietary wheat bran in exocrine pancreas and intestine of rats. Journal of Nutrition 112: 283–288.CrossRefGoogle ScholarPubMed
Simmonds, H. A., Rising, T. J., Cadenhead, A., Hatfield, P. J., Jones, A. S. and Cameron, J. S. 1973. Radioisotope studies of purine metabolism during administration of guanine and allopurinol in the pig. Biochemical Pharmacology 22: 2553–2563.CrossRefGoogle ScholarPubMed
Sorensen, L. B. 1978. Extrarenal disposal of uric acid. In Uric acid — handbook of experimental pharmacology (ed. Kelley, W. N. and Weiner, I. M.), pp. 325–336. Springer-Verlag, Berlin.CrossRefGoogle Scholar
Statistical Analysis Systems Institute. 1988. SAS user's guide, statistics. SAS Institute Inc., Cary, NC.Google Scholar
Steel, R. G. D. and Torrie, J. H. 1960. Principles and procedures of statistics. McGraw-Hill Book Company Inc., New York.Google Scholar
Storm, E., Brown, D. S. and Ørskov, E. R. 1983. The nutritive value of rumen micro-organisms in ruminants. 3. The digestion of microbial amino acid and nucleic acids in and losses of endogenous nitrogen from the small intestine. British Journal of Nutrition 50:479–485.CrossRefGoogle ScholarPubMed
Surra, J., Guada, J. A., Balcells, J. and Castrillo, C. 1997. Renal and salivary clearance of purine derivatives in sheep. Animal Science 65: 83–91.CrossRefGoogle Scholar
Taverner, M. R., Hume, I. D. and Farrell, D. J. 1981. Availability to pigs of amino acids in cereal grains. 1. Endogenous levels of amino acids in ileal digesta and faeces of pigs given cereal diets. British Journal of Nutrition 46: 149–158.CrossRefGoogle ScholarPubMed
Technicon Instruments Co. 1977. Technicon SMA II method no. SD4-00118k7. Technicon Instruments Co., Tarrytown, NY.Google Scholar
Warner, R. L., Mitchell, G. E. Jr and Little, C. O., 1972. Post-ruminal digestion of cellulose in wethers and steers. Journal of Animal Science 34:161–165.CrossRefGoogle Scholar
Zebrowska, T. 1985. The influence of the level and source of fiber in the diet on the exocrine pancreatic secretion in growing pigs. Proceedings of the third international seminar on digestive physiology in the pig, Copenhagen, pp. 152–154.Google Scholar