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Effects of dietary linseed on nutritional value and other quality aspects of pig muscle and adipose tissue

Published online by Cambridge University Press:  18 August 2016

P. A. Riley ,
M. Enser ,
G. R. Nute  and
J. D. Wood
P. A. Riley
Affiliation:
Division of Food Animal Science, Department of Clinical Veterinary Science, University of Bristol, Langford, Bristol BS40 5DU, UK
M. Enser
Affiliation:
Division of Food Animal Science, Department of Clinical Veterinary Science, University of Bristol, Langford, Bristol BS40 5DU, UK
G. R. Nute
Affiliation:
Division of Food Animal Science, Department of Clinical Veterinary Science, University of Bristol, Langford, Bristol BS40 5DU, UK
J. D. Wood*
Affiliation:
Division of Food Animal Science, Department of Clinical Veterinary Science, University of Bristol, Langford, Bristol BS40 5DU, UK
*
†Author for correspondence
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Abstract

This study reports improvements in the nutritional value of pork by including linseed in the diets of boar and gilt pigs. Two feeding strategies, either short-term or long-term, were employed: (i) 0 g or 114 g linseed per kg of food provided to 16 pigs of 87 kg live weight for 20 or 27 days; (ii) 0 g, 10 g, 20 g or 30 g linseed per kg of food provided to 64 pigs of 46 kg live weight for 54, 62, 68 or 75 days. All diets were supplemented with DL-α-tocopheryl acetate (0•2 g/kg). The 18 : 3n-3 contents (18 : 2n-6 :18 : 3n-3 ratios in parentheses) of the short-term 0 g and 114 g/kg, and long-term 0g, 10 g, 20 g and 30 g/kg linseed diets, were 2•1 g (7•75), 28•1 g (0•70), 1•0 g (8•86), 3•4 g (2•73), 6•0 g (1•66) and 8•1 g/kg food (1•25) respectively. The long-term 30 g/kg diet reduced the n-6 : n-3 ratio of muscle and adipose tissue as successfully as the short-term 114 g/kg diet to accord with guidelines for the overall human diet (5 :1 or less) but required only 0•73 as much linseed and increased the proportion of C20-22 n-3 fatty acids more effectively; compared with their respective control diets, the 114 g/kg and 30 g/kg diets more than halved the n-6 : n-3 ratios in muscle to 3•8 and 3•9, approximately trebled the concentrations of 18 : 3n-3 in muscle to 0•43 mg and 0•28 mg/g tissue, and doubled the concentrations of 20: 5n-3 in muscle to 0•08 mg and 0•10 mg/g tissue. Similar changes occurred in adipose tissue. With both strategies, the majority of the changes had occurred by the time the first groups were slaughtered. There was a strong relationship between the 18 : 2n-6 :18 : 3n-3 ratio of the food and tissues and the accumulation of all C20-22 n-3 fatty acids, except 22 : 6n-3, which was unaffected by dietary linseed. The improvements in nutritional value were obtained without changes in organoleptic characteristics, as measured by a trained taste panel, or significant loss of shelf-life, as measured by TBARS analysis and colour stability.

Keywords

dietary fatfatty acidslinseedmeat qualitypigs
Type
Research Article
Information
Animal Science , Volume 71 , Issue 3 , December 2000 , pp. 483 - 500
Copyright
Copyright © British Society of Animal Science 2000

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References

Ahn, D. U., Lutz, S. and Sim, J. S. 1996. Effects of dietary alpha-linolenic acid on the fatty acid composition, storage stability and sensory characteristics of pork loin. Meat Science 43: 291—299.CrossRefGoogle ScholarPubMed
Allen, E., Bray, R. W. and Cassens, R. G. 1967. Changes in fatty acid composition of porcine muscle lipid associated with sex and weight. Journal of Food Science 32: 26—29.Google Scholar
Bauer, J. E., Dunbar, B. L. and Bigley, K. E. 1998. Dietary flaxseed in dogs results in differential transport and metabolism of (n-3) polyunsaturated fatty acids. Journal of Nutrition 128: 2641S-2644S.Google Scholar
Blanchard, P. J., Ellis, M., Warkup, C. C., Chadwick, J. P. and Willis, M. B. 1999. The influence of sex (boars and gilts) on growth, carcass and pork eating quality characteristics. Animal Science 68: 487—493.Google Scholar
Botsford, L. W., Castilla, J. C. and Peterson, C. H. 1997. The management of fisheries and marine ecosystems. Science 277: 509—515.Google Scholar
Brenner, R. R. 1971. The desaturation step in the animal biosynthesis of polyunsaturated fatty acids. Lipids 6: 567—575.Google Scholar
British Nutrition Foundation. 1992. Unsaturated fatty acids: nutritional and physiological significance. Task Force report. Chapman and Hall, London.Google Scholar
British Standards Institution. 1984. Methods for sensory analysis of food, BS5929. Part 3. Triangular test. British Standards Institution, London, UK.Google Scholar
Brooks, C. C. 1971. Fatty acid composition of pork lipids as affected by basal diet, fat source and fat level. Journal of Animal Science 33: 1224—1231.Google Scholar
Cameron, N. D. and Enser, M. 1991. Fatty acid composition of lipid in longissimus dorsi muscle of Duroc and British Landrace pigs and its relationship with eating quality. Meat Science 29: 295—307.Google Scholar
Cherian, G. and Sim, J. S. 1995. Dietary alpha-linolenic acid alters the fatty acid composition of lipid classes in swine tissues. Journal of Agricultural and Food Chemistry 43: 2911—2916.Google Scholar
Cunnane, S. C., Stitt, P. A., Ganguli, S. and Armstrong, J. K. 1990. Raised omega-3 fatty acid levels in pigs fed flax. Canadian Journal of Animal Science 70: 251—254.CrossRefGoogle Scholar
Dahl, O. and Persson, K. 1965. Properties of animal depot fat in relation to dietary fat. Journal of the Science of Food and Agriculture 16: 452—455.Google Scholar
Department of Health. 1994. Nutritional aspects of cardiovascular disease. Report on health and social subjects, no. 46. Her Majesty’s Stationery Office, London.Google Scholar
Department of Trade and Industry. 1997. Foresight for food and drink: meat. Department of Trade and Industry, UK.Google Scholar
Enser, M. 1995. Meat lipids. In Developments in oils and fats (ed. Hamilton, R. J.), pp. 1—32. Blackie Academic and Professional, Glasgow.Google Scholar
Enser, M., Hallett, K., Hewitt, B., Fursey, G. A. J. and Wood, J. D. 1996. Fatty acid content and composition of English beef, lamb and pork at retail. Meat Science 42: 443—456.Google Scholar
Enser, M., Wood, J. D., Richardson, R. I., Gill, B. P. and Sheard, P. R. 2000. Feeding linseed to increase the n-3 PUFA of pork: fatty acid composition of muscle, adipose tissue, liver and sausages. Meat Science 55: 201—212.Google Scholar
Folch, J., Lees, M. and Sloane-Stanley, G. H. 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226: 497—509.Google Scholar
Fontanillas, R., Barroeta, A., Baucells, M. D. and Guardiola, F. 1998. Backfat fatty acid evolution in swine fed diets high in either cis-monounsaturated, trans, or (n-3) fats. Journal of Animal Science 76: 1045—1055.Google Scholar
Food and Agriculture Organization/World Health Organization. 1994. Fats and oils in human nutrition Report of a joint expert consultation, food and nutrition paper 57. Food and Agriculture Organization, Rome.Google Scholar
Gerster, H. 1998. Can adults adequately convert a- linolenic acid (18 : 3n-3) to eicosapentaenoic acid (20 : 5n-3) and docosahexaenoic acid (22 : 6n-3)? International Journal for Vitamin and Nutrition Research 68: 159—173.Google Scholar
Gray, J. I. and Pearson, A. M. 1987. Rancidity and warmed-over flavour. In Advances in meat research, vol. 3, restructured meat and poultry products (ed. Pearson, A. M. and Dutson, T. R.), pp. 221—269. Van Nostrand Reinhold Co., New York.Google Scholar
Jacobs, M. N., Johnston, P. A., Wyatt, C. L., Santillo, D. and French, M. C. 1997. Organochlorine pesticide and PCB residues in pharmaceutical, industrial and food grade fish oils. International Journal of Environment and Pollution 8: 74—93.Google Scholar
Kearney, J. M. and McElhone, S. 1999. Perceived barriers in trying to eat healthier — results of a pan-EU consumer attitudinal survey. British Journal of Nutrition 81: S133-S137.Google Scholar
Koch, D. E., Pearson, A. M., Magee, W. T., Hoefer, J. A. and Schweigert, B. S. 1968. Effect of diet on the fatty acid composition of pork fat. Journal of Animal Science 27: 360—365.Google Scholar
Leece, E. A. and Allman, M. A. 1996. The relationship between dietary a- linolenic: linoleic acid and rat platelet eicosapentaenoic and arachidonic acids. British Journal of Nutrition 76: 447—452.Google Scholar
Leskanich, C. O., Matthews, K. R., Warkup, C. C., Noble, R. C. and Hazzledine, M. 1997. The effect of dietary oil containing (n-3) fatty acids on the fatty acid, physicochemical, and organoleptic characteristics of pig meat and fat. Journal of Animal Science 75: 673—683.Google Scholar
Li, D., Ng, A., Mann, N. J. and Sinclair, A. J. 1998. Contribution of meat fat to dietary arachidonic acid. Lipids 33: 437—440.Google Scholar
Malmfors, B., Lundström, K. and Hansson, I. 1978. Fatty acid composition of porcine back fat and muscle lipids as affected by sex, weight and anatomical location. Swedish Journal of Agricultural Research 8: 25—38.Google Scholar
Morgan, C. A., Noble, R. C., Cocchi, M. and McCartney, R. 1992. Manipulation of the fatty acid composition of pig meat lipids by dietary means. Journal of the Science of Food and Agriculture 58: 357—368.Google Scholar
Newton, I. S. 1998. Global food fortification perspectives of long-chain w 3 fatty acids. In The return of w 3 fatty acids into the food supply. I. Land-based animal food products and their health effects. World review of nutrition and dietetics, vol. 83, pp. 199—209. Karger, Basel.Google Scholar
Oeckel, M. J. van, Casteels, M., Warnants, N., Damme, L. van and Boucqué, Ch. V. 1996. Omega-3 fatty acids in pig nutrition: implications for the intrinsic and sensory quality of the meat. Meat Science 44: 55—63.Google Scholar
Otten, W., Wirth, C., Iaizzo, P. A. and Eichinger, H. M. 1993. A high omega-3 fatty acid diet alters fatty acid composition of heart, liver, kidney, adipose tissue and skeletal muscle in swine. Annals of Nutrition and Metabolism 37: 134—141.Google Scholar
Rodriguez, A., Sarda, P. , Boulot, P. , Leger, C. L. and Descomps, B. 1999. Differential effect of N-ethyl maleimide on D 6-desaturase activity in human fetal liver toward fatty acids of the n-6 and n-3 series. Lipids 34: 23—30.Google Scholar
Romans, J. R., Johnson, R. C., Wulf, D. M., Libal, G. W. and Costello, W. J. 1995a. Effects of ground flaxseed in swine diets on pig performance and on physical and sensory characteristics and omega-3 fatty acid content of pork. I. Dietary level of flaxseed. Journal of Animal Science 73: 1982—1986.CrossRefGoogle ScholarPubMed
Romans, J. R., Wulf, D. M., Johnson, R. C., Libal, G. W. and Costello, W. J. 1995b. Effects of ground flaxseed in swine diets on pig performance and on physical and sensory characteristics and omega-3 fatty acid content of pork. II. Duration of 15% dietary flaxseed. Journal of Animal Science 73: 1987—1999.Google Scholar
Rustan, A. C., Nossen, J. O., Osmundsen, H. and Drevon, C. A. 1988. Eicosapentaenoic acid inhibits cholesterol esterification in cultured parenchymal cells and isolated microsomes from rat liver. Journal of Biological Chemistry 263: 8126—8132.Google Scholar
Simopoulos, A. P. 1999. Essential fatty acids in health and chronic disease. American Journal of Clinical Nutrition 70: S560-S569.Google Scholar
Simopoulos, A. P., Leaf, A. and Salem, N. 1999. Workshop on the essentiality of and recommended dietary intakes for omega-6 and omega-3 fatty acids. Available at: http://www.issfal.org.uk/adequateintakes.htm Google Scholar
SpechtOverholt, S., Romans, J. R., Marchello, M. J., Izard, R. S., Crews, M. G., Simon, D. M., Costello, W. J. and Evenson, P. D. 1997. Fatty acid composition of commercially manufactured omega-3 enriched pork products, haddock and mackerel. Journal of Animal Science 75: 2335—2343.Google Scholar
Sprecher, H., Luthria, D. L., Mohammed, B. S. and Baykousheva, S. P. 1995. Reevaluation of the pathways for the biosynthesis of polyunsaturated fatty acids. Journal of Lipid Research 36: 2471—2477.Google Scholar
Tarladgis, B. G., Watts, B. M., Younathan, M. T. and Dugan, L. 1960. A distillation method for quantitative determination of malonaldehyde in rancid foods. Journal of the American Oil Chemists Society 37: 44—48.Google Scholar
Vyncke, W. 1975. Evaluation of the direct thiobarbituric acid extraction method for determining oxidative rancidity in mackerel (Scomber scombrus L .) Fette Seifen Anstrichmittel 77: 239—240.Google Scholar
Warnants, N., Oekel, M. J. van and Boucqué, Ch. V. 1998. Effect of incorporation of dietary polyunsaturated fatty acids in pork backfat on the quality of salami. Meat Science 49: 435—445.Google Scholar
Warriss, P. D. 1996. Instrumental measurement of colour. In Meat quality and meat packaging (ed. Taylor, S. A., Raimundo, A., Severini, M. and Smulders, F.J. M.), pp. 221—232. European Consortium for Continuing Education in Advanced Meat Science, Utrecht.Google Scholar
Whittington, F. M., Prescott, N. J., Wood, J. D. and Enser, M. 1986. The effect of dietary linoleic acid on the firmness of backfat in pigs of 85 kg live weight. Journal of the Science of Food and Agriculture 37: 753—761.Google Scholar
Wood, J. D. and Enser, M. 1997. Factors influencing fatty acids in meat and the role of antioxidants in improving meat quality. British Journal of Nutrition 78: S49—S60.Google Scholar
Wood, J. D., Enser, M., Fisher, A. V., Nute, G. R., Richardson, R. I. and Sheard, P. R. 1999. Manipulating meat quality and composition. Proceedings of the Nutrition Society 58: 1–8.Google Scholar
Wood, J. D., Enser, M., Whittington, F. M., Moncrieff, C. B. and Kempster, A. J. 1989. Backfat composition in pigs: differences between fat thickness groups and sexes. Livestock Production Science 22: 351—362.Google Scholar
Wood, J. D., Jones, R. C. D., Francombe, M. A. and Whelehan, O. P. 1986. The effects of fat thickness and sex on pig meat quality with special reference to the problems associated with overleanness. 2. Laboratory and trained taste panel results. Animal Production 43: 535—544.Google Scholar