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Effects of age and maternal reactivity on the stress response of the pituitary-adrenocortical axis and the sympathetic nervous system in neonatal pigs

Published online by Cambridge University Press:  18 August 2016

E. Kanitz ,
W. Otten ,
G. Nürnberg  and
K. P. Brüssow
E. Kanitz
Affiliation:
Department of Physiological Principles in Animal Behaviour, Research Institute for the Biology of Farm Animals, 18196 Dummerstorf, Germany
W. Otten
Affiliation:
Department of Physiological Principles in Animal Behaviour, Research Institute for the Biology of Farm Animals, 18196 Dummerstorf, Germany
G. Nürnberg
Affiliation:
Department of Biometry, Research Institute for the Biology of Farm Animals, 18196 Dummerstorf, Germany
K. P. Brüssow
Affiliation:
Department of Reproductive Biology, Research Institute for the Biology of Farm Animals, 18196 Dummerstorf, Germany
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Abstract

The study was conducted to investigate the adreno cortical capacity after injection of ACTH and the sensitivity of the pituitary and the adrenal to immobilization in neonatal pigs at different ages. Furthermore, the endocrine reactivity of the offspring was compared with the stress reactivity of their mothers. Four piglets were selected from each of six different litters and subjected to an immobilization test and an adrenal function test using synthetic ACTH1-24 at the ages of 7, 21 and 35 days; the six sows were also subjected to restraint and an ACTH stimulation test. Plasma β-endorphin, norepinephrine and epinephrine concentrations were measured in blood samples taken 2 min after restraint and cortisol concentrations were measured 60 min after ACTH administration. A highly sensitive adrenal response was demonstrated in both sows and piglets and adrenal reactivity showed also a considerable consistency over time within sows. In neonatal pigs, the cortisol response to ACTH was greatest on day 7 and decreased up to day 35. Plasma epinephrine and norepinephrine levels after the 2-min immobilization were also higher at day 7 compared with the other ages (P < 0·01). Piglets from sows, classified as high reacting according to their cortisol or epinephrine response, also showed significantly higher cortisol levels after ACTH challenge at all ages and significantly higher epinephrine levels after restraint at day 7 than piglets from low reacting sows. The results show an age-related change of pituitary-adrenocortical and sympatho-adrenomedullary responses in neonatal pigs and an absence of a stress hyporesponsive period at all ages studied. The results also indicate different levels of excitability in the offspring depending on the maternal stress reactivity.

Keywords

catecholamineshydrocortisoneneonatespigsstress
Type
Research Article
Information
Animal Science , Volume 68 , Issue 3 , April 1999 , pp. 519 - 526
Copyright
Copyright © British Society of Animal Science 1999

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References

Bargmann, W. 1977. Histologie und mikroskopische Anatomie des Menschen. 7. Aufl Georg Thieme, Stuttgart.Google Scholar
Blatchford, D., Holzbauer, M. and Ingram, D. L. 1978. Responses of the pituitary-adrenal system of the pig to environmental changes and drugs. British Journal of Pharmacology 62: 241–254.CrossRefGoogle ScholarPubMed
Borell, E. von and Ladewig, J. 1989. Altered adrenocortical response to acute Stressors or ACTH (1-24) in intensively housed pigs. Domestic Animal Endocrinology 6: 299–309.CrossRefGoogle Scholar
Borell, E. von and Ladewig, J. 1992. Relationship between behaviour and adrenocortical response pattern in domestic pigs. Applied Animal Behaviour Science 34: 195–206.CrossRefGoogle Scholar
Bradshaw, R. H., Parrott, R. F., Forsling, J. A., Goode, J. A., Lloyd, D. M., Rodway, R. G. and Broom, D. M. 1996a. Stress and travel sickness in pigs: effects of road transport on plasma concentrations of cortisol, beta-endorphin and lysine vasopressin. Animal Science 63: 507–516.Google Scholar
Bradshaw, R. H., Parrott, R. F., Goode, J. A., Lloyd, D. M., Rodway, R. G. and Broom, D. M. 1996b. Behavioural and hormone responses of pigs during transport: effect of mixing and duration of journey. Animal Science 62: 547–554.Google Scholar
Geers, R. 1995. A modulating effect of ß-endorphin on pigs’ coping strategies during transport. Physiology and Behavior 57: 1057–1060.CrossRefGoogle Scholar
Guillet, R., Saffran, M. and Michaelson, S. M. 1980. Pituitary-adrenal response in neonatal rats. Endocrinology 106: 991–994.Google Scholar
Hennessy, D. P., Stemasiak, T. and Johnson, N. E. 1988. Consistent capacity for adrenocortical response to ACTH administration in pigs. American Journal of Veterinary Research 49: 1276–1283.Google Scholar
Janssens, C. J. J.G., Helmond, F. A. and Wiegant, V. M. 1994. Increased cortisol response to exogenous adrenocorticotropic hormone in chronically stressed pigs: influence of housing conditions. Journal of Animal Science 72: 1771–1777.CrossRefGoogle ScholarPubMed
Jensen, K. H., Hansen, S. W. and Pedersen, L. J. 1996. The effect of long-term stress on the hypothalamic-pituitary-adrenocortical axis and the role of the Stressors. Acta Agricultura Scandinavica, Section A, Animal Science Supplementum 27: 40–45.Google Scholar
Jensen, K. H., Pedersen, L. J., Giersing Hageiso, A. M., Heller, K., Jorgensen, E. and Ladewig, J. 1995. Intermittent stress in pigs: behavioural and pituitary-adrenocortical reactivity. Acta Agricultura Scandinavica, Section A, Animal Science 45: 276–285.Google Scholar
Klemcke, H. G. 1994. Response of the porcine pituitary-adrenal axis to chronic intermittent Stressor. Domestic Animal Endocrinology 11: 133–149.Google Scholar
Klemcke, H. G., Brown-Borg, H. M. and Borg, K. E. 1995. Functioning of the porcine pituitary-adrenocortical axis during neonatal development. Biology of the Neonate 67: 274–286.CrossRefGoogle ScholarPubMed
Klemcke, H. G. and Pond, W. G. 1991. Porcine adrenal adrenocorticotropic hormone receptors: characterization, changes during neonatal development, and response to a Stressor. Endocrinology 128: 2478–2488.Google Scholar
Kloet, E. R. de, Rosenfeld, P., Eekelen, J. A. M. van, Sutanto, W. and Levine, S. 1988. Stress, glucocorticoids and development in rats. Progress in Brain Research 73: 101–120.Google Scholar
Kloet, E. R.de, Rots, N. Y. and Cools, A. R. 1996. Brain-corticosteroid hormone dialogue: slow and persistent. Cellular and Molecular Neurobiology 16: 345–356.Google Scholar
Malone, S. M., Gunnar, M. R. and Fisch, R. O. 1985. Adrenocortical and behavioral responses to limb restraint in human neonates. Development in Psychobiology 18: 435–446.Google Scholar
Manteuffel, G. and Puppe, B. 1997. Is it possible to judge the subjective perception of internal state in animals? A critical analysis from a scientific point of view. Archiv für Tierzucht 40: 109–121.Google Scholar
Moberg, G. P., Anderson, C.O. and Underwood, T. R. 1980. Ontogeny of the adrenal and behavior responses in lambs to emotional stress. Journal of Animal Science 51: 138–142.Google Scholar
Otten, W., Puppe, B., Stabenow, B., Kanitz, E., Schön, P.C., Brüssow, K.P., and Nürnberg, G. 1997. Agonistic interactions and physiological reactions of top and bottom ranking pigs confronted with a familiar and unfamiliar group: preliminary results. Applied Animal Behaviour Science 55: 79–90.Google Scholar
Sapolsky, R. M. and Meaney, M. J. 1986. Maturation of the adrenocortical stress response: neuroendocrine control mechanism and the stress hyporesponsive period. Brain Research Review 11: 65–76.Google Scholar
Schapiro, S., Geller, E. and Eiduson, S. 1962. Neonatal adrenal cortical response to stress and vasopressin. Proceedings of the Society of Experimental Biology and Medicine 109: 937–941.CrossRefGoogle ScholarPubMed
Sebranek, J. G., Marple, D. N. and Cassens, R. G. 1973. Adrenal response to ACTH in the pig. Journal of Animal Science 36: 41–44.CrossRefGoogle ScholarPubMed
Statistical Analysis Systems Institute. 1989. SAS/STAT user’s guide, version 6, fourth edition, volume 2. SAS Institute, Cary, NC.Google Scholar
Walker, C.-D., Perrin, M., Vale, W. and Rivier, C. 1986. Ontogeny of the stress response in the rat: role of the pituitary and the hypothalamus. Endocrinology 118: 1445–1451.Google Scholar
Walker, C.-D., Scribner, K. A., Cascio, C. S. and Dallman, M. F. 1991. The pituitary-adrenocortical system of neonatal rats is responsive to stress throughout development in a time-dependent and stressor-specific fashion. Endocrinology. 128:1385–1395.Google Scholar
Widmaier, E. P. 1990. Glucose homeostasis and hypothalamic-pituitary-adrenocortical axis during development in rats. American Journal of Physiology 259: E601–E613.Google Scholar
Witek-Janusek, L. 1988. Pituitary-adrenal response to bacterial endotoxin in developing rats. American Journal of Physiology 255: E525–E530.Google Scholar
Yamaguchi, I. and Kopin, I. J. 1979. Plasma catecholamine and blood pressure responses to sympathetic stimulation in pithed rats. American Journal of Physiology 237: H305–H310.Google Scholar
Zhang, S. H., Hennessy, D. P. and Cranwell, P. D. 1990a. In vitro study of the function of adrenocortical cells from pigs of differing in vivo response to adrenocorticotropin. American Journal of Veterinary Research 51: 1016–1020.Google Scholar
Zhang, S. H., Hennessy, D. P. and Cranwell, P. D. 1990b. Pituitary and adrenocortical response to corticotropin-releasing factor in pigs. American Journal of Veterinary Research 51:1020–1024.Google Scholar
Zhang, S. H., Hennessy, D. P., Cranwell, P. D., Noonan, D. E. and Francis, H. J. 1993. Physiological responses to exercise and hypoglycaemia stress in pigs of differing adrenal responsiveness. Comparative Biochemistry and Physiology 103: 695–703.Google Scholar