Hostname: page-component-745bb68f8f-5r2nc Total loading time: 0 Render date: 2025-01-12T13:49:54.419Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of storage time and number of spermatozoa per insemination dose on semen characteristics and fertilizing capacity of boar semen diluted with Beltsville Thaw Solution (BTS) extender

Published online by Cambridge University Press:  02 September 2010

C. Alexopoulos ,
C. Boscos ,
Ph. Saratsis ,
C. Saoulidis  and
S. Kyriakis
C. Alexopoulos
Affiliation:
Clinic of Obstetrics and Artificial Insemination, Faculty of Veterinary Medicine, Aristotelian University of Thessaloniki, GR 54006 Thessaloniki, Greece
C. Boscos
Affiliation:
Clinic of Obstetrics and Artificial Insemination, Faculty of Veterinary Medicine, Aristotelian University of Thessaloniki, GR 54006 Thessaloniki, Greece
Ph. Saratsis
Affiliation:
Clinic of Obstetrics and Artificial Insemination, Faculty of Veterinary Medicine, Aristotelian University of Thessaloniki, GR 54006 Thessaloniki, Greece
C. Saoulidis
Affiliation:
Clinic of Medicine, Faculty of Veterinary Medicine, Aristotelian University of Thessaloniki, GR 54006 Thessaloniki, Greece
S. Kyriakis
Affiliation:
Clinic of Medicine, Faculty of Veterinary Medicine, Aristotelian University of Thessaloniki, GR 54006 Thessaloniki, Greece
Get access

Abstract

Semen collection from three boars was performed twice a week, and doses (100 ml each) of semen diluted with Beltsville Thaw Solution (BTS) extender were prepared containing 1 × 109, 3 × 109 and 5 × 109 spermatozoa. Diluted semen was then stored at 17°C for a maximum of 120 h. Percentage of motile spermatozoa (PMS) and type of spermatozoa motility (TSM) were determined by microscopic examinations performed at 24-h intervals, from zero time (beginning of storage at 17°C) up to 120 h of storage, and a sperm motility index (SMI) calculated. Sows (no. = 360) were divided into nine experimental groups (40 sows per group) and a combination of number of spermatozoa per dose and storage time was used for artificial insemination in each group. All the animals were inseminated twice at 12 and 36 h after detection of standing oestrus by a teaser boar. Return and farrowing data including litter size were recorded. PMS, TSM and SMI decreased significantly (P < 0·01) from 48 h up to 120 h of storage, this being more marked in semen doses of 5 × 109 spermatozoa. When semen stored for up to 24 h was used, no significant differences (P > 0·05) regarding return rates and farrowing rates were observed among the three groups inseminated with semen containing different numbers of spermatozoa per dose. Inseminations with semen doses of 1 × 109 and 3 × 109 spermatozoa stored for 48 to 72 h have significantly lower return rates (P < 0·05) and significantly higher farrowing rates (P < 0·05) than with doses of 5 × 109 spermatozoa. Inseminations with semen stored for more than 72 h gave relatively higher return rates and lower farrowing rates. Semen storage time and number of spermatozoa per dose appeared to reduce litter size only where semen doses of 5 X109 spermatozoa were inseminated after storage for more than 48 h.

Keywords

boarssemen diluentsspermatozoastorage life
Type
Research Article
Information
Animal Science , Volume 62 , Issue 3 , June 1996 , pp. 599 - 604
Copyright
Copyright © British Society of Animal Science 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aalbers, J. G., Johnson, L. A., Rademaker, J. H. M. and Grooten, H. J. G. 1984. Use of boar spermatozoa for Al: fertility and morphology of semen diluted in BTS and used for insemination within 24 hrs or 25 to 48 hrs after collection. Proceedings of the tenth international congress on animal reproduction and artificial insemination, Urbana, Illinois, vol. 11, no. 180, pp.1–3.Google Scholar
Aalbers, J. G., Rademaker, J. H. M., Grooten, H. J. G. and Johnson, L. A. 1983. Fecundity of boar semen stored in BTS, Kiev, Zorlesco and Modena extenders under field conditions. journal of Animal Science 57:Suppl. 1, pp.314–315.Google Scholar
Johnson, L. A. and Aalbers, J. G. 1984. Artificial insemination of swine: fertility using several liquid semen diluents. Proceedings of the eighth international pig veterinary society congress, Ghent, Belgium p. 293.Google Scholar
Johnson, L. A., Aalbers, J. G. and Grooten, H. J. G. 1988. Artificial insemination of swine: fecundity of boar semen stored in Beltsville TS (BTS), Modified Moden a (MM), or MR-A and inseminated on one, three and four days after collection. Zuchthygiene 23:49–55.CrossRefGoogle Scholar
Johnson, L. A., Aalbers, J. G., Willems, C. M. T., Rademaker, J. H. M. and Rexroad, C. E. 1982. Use of boar spermatozoa for artificial insemination. III. Fecundity of boar spermatozoa stored in Beltsville liquid and Kiev extenders for 3 days at 18°C. journal of Animal Science 54:132–136.CrossRefGoogle Scholar
Johnson, L. A., Aalbers, J. G., Willems, C. M. T. and Sybesma, W. 1981. Use of boar spermatozoa for artificial insemination. I. Fertilizing capacity of fresh and frozen spermatozoa in sows on 36 farms, journal of Animal Science 52:1130–1136.CrossRefGoogle ScholarPubMed
King, G. J. and Macpherson, J. W. 1973. A comparison of two methods for boar semen collection. journal of Animal Science 36:563–565.CrossRefGoogle ScholarPubMed
Machaty, Z., Takacs, T. and Gathy, I. 1992. Fertilizing capacity of boar semen diluted with Beltsville TS (BTS) and Modified Kiev (MK) extenders in relation to storage time and number of spermatozoa per insemination dose. Animal Reproduction Science 29:289–295.CrossRefGoogle Scholar
Reed, H. C. B. 1982. Commercial requirements for an effective fresh semen diluent. Proceedings of the second international conference on hoar semen preservation, 19-22 August, 1990, Beltsville, MD pp.255–270.Google Scholar
Reed, H. C. B. 1990. Artificial insemination. In Control of pig reproduction (ed. Cole, D. J. A. and Foxcroft, D.), pp.65–90. Butterworths, London.Google Scholar
Revell, S. G. and Glossop, C. E. 1989. A long-time ambient temperature diluent for boar semen. Animal Production 48:579–584.CrossRefGoogle Scholar
Waberski, D. 1988. [In vitro experiments and field trial of fertilization using long-term-stored liquid boar semen with special reference to the BSA and the buffer in the diluent]. Ph.D. thesis, University of Hannover.Google Scholar
Waberski, D., Dirksen, G., Weitze, K. F., Leiding, C. and Willmen, D. 1990. Effect of semen quality on fertility results of stored liquid boar semen — a field trial. Proceedings of the second international conference on boar semen preservation, 19-22 August, 1990, Beltsville, MD pp.375–378.Google Scholar
Waberski, D., Meding, S., Dirksen, G., Weitze, K. F., Leiding, C. and Hahn, R. 1994a. Fertility of long-term-stored boar semen: influence of extender (Androhep and Kiev), storage time and plasma droplets in the semen. Animal Reproduction Science 36:145–151.CrossRefGoogle Scholar
Waberski, D., Weitze, K. F., Lietmann, C., Liibbert zur Lage, W., Bortolozzo, F. P., Willmen, T. and Petzoldt, R. 1994b. The initial fertilizing capacity of long term-stored liquid boar semen following pre- and postovulatory insemination. Theriogenology 41:1367–1377.CrossRefGoogle Scholar
Weitze, K. F. 1990a. Long-term storage of extended boar semen. Proceedings of the second international conference on boar semen preservation, 19-22 August, 1990, Beltsville, MD pp.231–253.Google Scholar
Weitze, K. F. 1990b. The use of “long-term extender” in pig AI — a view of the international situation. Pig News and Information 11:23–26.Google Scholar