Hostname: page-component-78c5997874-lj6df Total loading time: 0 Render date: 2024-11-03T05:18:30.147Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of dietary lipid on the content of conjugated linoleic acid (CLA) in beef muscle

Published online by Cambridge University Press:  18 August 2016

M. Enser ,
N. D. Scollan ,
N. J. Choi ,
E. Kurt ,
K. Hallett  and
J. D. Wood
M. Enser
Affiliation:
Division of Food Animal Science, University of Bristol, Langford, Bristol BS40 5DU
N. D. Scollan
Affiliation:
Institute of Grassland and Environmental Research, Plas Goggerdan, Aberystwyth SY23 3EB
N. J. Choi
Affiliation:
Institute of Grassland and Environmental Research, Plas Goggerdan, Aberystwyth SY23 3EB
E. Kurt
Affiliation:
Division of Food Animal Science, University of Bristol, Langford, Bristol BS40 5DU
K. Hallett
Affiliation:
Division of Food Animal Science, University of Bristol, Langford, Bristol BS40 5DU
J. D. Wood
Affiliation:
Division of Food Animal Science, University of Bristol, Langford, Bristol BS40 5DU
Get access

Abstract

We have examined the effect of dietary fats containing n-3 polyunsaturated fatty acids on the conjugated linoleic acid (CLA) content of beef m. longissimus lumborum. Four groups of eight Charolais steers were given, for 120 days, grass silage plus a barley/sugar-beet feed concentrate containing one of four fat supplements: Megalac (saturated), linseed (high 18: 3),fish oil (high 20: 5 n-3, eicosapentenoic acid and 22: 6 n-3, docosahexaenoic acid) or linseed plus fish oil. The concentrates supplied 400 g/kg dry-matter (DM) intake and were designed to supply 45 g/kg of the total dietary fat calculated to be 60 g/kg of DM and to contain similar amounts of linoleic acid. Muscle from steers given the Megalac supplement contained 11·3 mg CLA per 100 g muscle and this was increased two- to three-fold in animals given the more unsaturated fat supplements. The increased deposition of CLA was similar for both linseed and fish oil supplements although the concentrations of total n-3 polyunsaturated fatty acids in the fish oil diet were much less than in the linseed diet. This suggests potent inhibition of conversion of CLA to trans vaccenic acid by fish oil fatty acids or their rumen metabolites.

Keywords

beeffatty acidshydrogenationlinoleic acidrumen
Type
Research Article
Information
Animal Science , Volume 69 , Issue 1 , August 1999 , pp. 143 - 146
Copyright
Copyright © British Society of Animal Science 1999

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Chin, S. F., Liu, W., Storkson, J. M., Ha, Y. L. and Paríža, M. W. 1992. Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognised class of anticarcinogens. Journal of Food Composition and Analysis 5: 185–187.CrossRefGoogle Scholar
Choi, N. J., Kim, E. J., Maeng, W. J., Neville, M. A., Enser, M., Wood, J. D. and Scollan, N. D. 1997. Rumen biohydrogenation of fatty acids from different sources of fat. Proceedings of the British Society of Animal Science, 1997, p. 19.Google Scholar
Cook, M. E., Miller, C.C., Park, Y. and Paríža, M. 1993. Immune modulation by altered nutrient metabolism: nutritional control of immune-induced growth depression. Poultry Science 72: 1301–1305.10.3382/ps.0721301CrossRefGoogle ScholarPubMed
Czerkawski, J. W., Christie, W. W., Breckenridge, G. and Hunter, M. C. 1975. Changes in the rumen metabolism of sheep given increasing amounts of linseed oil in their diet. British Journal of Nutrition 34: 25–44.Google ScholarPubMed
Enser, M., Hallett, K., Hewett, B., Fursey, G. A. J. and Wood, J. D. 1996. Fatty acid content and composition of English beef, lamb and pork at retail. Meat Science 42: 443–456.Google Scholar
Feldman, E. B., Kris-Etherton, P.M, Kritchevsky, D. and Lichtenstein, J. H. 1996. Position paper on trans fatty acids. American Journal of Clinical Nutrition 63: 663–670.Google Scholar
Folch, J., Lees, M. and Stanley, G. H. S. 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226: 497–509.CrossRefGoogle Scholar
Fritsche, J. and Steinhart, H. 1998. Amounts of conjugated linoleic acid (CLA) in German foods and evaluation of daily intake. Zeitschrift für Lebensmittel-Untersuchung und Forschung A 206: 77–82.CrossRefGoogle Scholar
Garton, G. A. 1960. Fatty acid composition of the lipids of pasture grasses. Nature 187: 511–512.CrossRefGoogle Scholar
Ha, Y. L., Grimm, N. K. and Pariza, M. W. 1989. Newly recognised anticarcinogenic fatty acids: identification in natural and processed cheeses. Journal of Agricultural and Food Chemistry 37: 75–81.Google Scholar
Ha, Y. L., Storkson, J. and Pariza, M. 1990. Inhibition of benzo (a) pyrene induced mouse forestomach reoplasia by conjugated dienoic derivatives of linoleic acid. Cancer Research 50: 1097–1101.Google ScholarPubMed
Ip, C., Singh, M., Thompson, H. J. and Scimeca, J. A. 1994. Conjugated linoleic acid suppresses mammary carcinogenesis and proliferative activity of the mammary gland in the rat. Cancer Research 54: 1212–1215.Google ScholarPubMed
Jahreis, G., Fritsche, J. and Steinhart, H. 1997. Conjugated linoleic acid in milk fat — high variation depending on production system. Nutrition Research 17:1479–1484.CrossRefGoogle Scholar
Jiang, J., Bjoerck, L., Fonden, R. and Emanuelson, M. 1996. Occurrence of conjugated cis-9, trans-11 octadecadienoic acid in bovine milk: effects of feed and dietary regimen. Journal of Dairy Science 79: 435–445.CrossRefGoogle ScholarPubMed
Kelly, M. L., Berry, J. R., Dwyer, D. A., Griinari, J. M., Chouinard, P. Y., Amburgh, M. E. van and Bauman, D. E. 1998. Dietary fatty acid sources affect conjugated linoleic acid concentrations in milk from lactating dairy cows. Journal of Nutrition 128: 881–885.CrossRefGoogle Scholar
Kepler, C. R. and Tove, S. B. 1967. Biohydrogenation of unsaturated fatty acids. Journal of Biological Chemistry 242: 5686–5692.10.1016/S0021-9258(18)99355-5CrossRefGoogle Scholar
Knekt, P., Jarvinen, R., Seppanen, R., Pukkala, E. and Aromaa, A. 1996. Intake of dairy products and the risk of breast cancer. British Journal of Cancer 73: 687–691.10.1038/bjc.1996.119CrossRefGoogle ScholarPubMed
Kramer, J. K. G., Fellner, V., Dugan, M. E. R., Sauer, F. D., Mossoba, M. N. and Yurawecz, M. P. 1997. Evaluating acid and base catalysts in the methylation of milk and rumen fatty acids with special emphasis on conjugated dienes and total trans fatty acids. Lipids 32: 1219–1228.Google Scholar
Lawes Agricultural Trust. 1994. Genstat 5 reference manual. Rothamsted Experimental Station, Harpenden.Google Scholar
Lee, K. N., Kritchevsky, D. and Pariza, M. W. 1994. Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108:19–25.Google ScholarPubMed
Park, Y., Albright, K. J., Liu, W., Storkson, J. M., Cook, M. E. and Pariza, M. W. 1997. Effect of conjugated linoleic acid on body composition in mice. Lipids 32: 853–858.Google Scholar
Parodi, P. W. 1977. Conjugated dienoic acids of milk fat. Journal of Dairy Science 60:1550–1553.CrossRefGoogle Scholar
Scollan, N. D., Fisher, W. J., Davies, D. W. R., Fisher, A. V., Enser, M. and Wood, J. D. 1997. Manipulating the fatty acid composition of muscle in beef cattle. Proceedings of the British Society of Animal Science, 1997, p. 20.Google Scholar
Shantha, N. C., Crum, A. D. and Decker, E. A. 1994. Evaluation of conjugated linoleic acid concentrations in cooked beef. Journal of Agricultural and Food Chemistry 42: 1757–1760.Google Scholar
Shantha, N. C., Moody, W. G. and Tabeidi, Z. 1997. Conjugated linoleic acid concentration in semimembranosus muscle of grass- and grain-fed and zeranol implanted beef cattle. Journal of Muscle Foods 8: 105–110.CrossRefGoogle Scholar
Stanton, C., Lawless, F., Kjellmer, G., Harrington, D., Devery, R., Connolly, J. F. and Murphy, J. 1997. Dietary influences on bovine milk cis-9, 11-trans conjugated linoleic acid content. Journal of Food Science 62:1083–1086.CrossRefGoogle Scholar
Willett, W. C., Stampfer, M. J., Manson, J. E., Colditz, G. A., Speizer, F. E., Rozner, B. A., Sampson, L. A. and Hennekins, C. H. 1993. Intake of trans fatty acids and the risk of coronary heart disease among women. Lancet 341: 581–585.CrossRefGoogle Scholar