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Detection of quantitative trait loci for locomotion and osteochondrosis-related traits in Large White ✕ Meishan pigs

Published online by Cambridge University Press:  18 August 2016

G.J. Lee ,
A.L. Archibald ,
G.B. Garth ,
A.S. Law ,
D. Nicholson ,
A. Barr  and
C.S. Haley
G.J. Lee
Affiliation:
Roslin Institute, Roslin, Midlothian, EH25 9PS, UK
A.L. Archibald
Affiliation:
University of Bristol, Division of Companion Animal Studies, Langford, Bristol, BS40 5DU, UK
G.B. Garth
Affiliation:
Roslin Institute, Roslin, Midlothian, EH25 9PS, UK
A.S. Law
Affiliation:
Roslin Institute, Roslin, Midlothian, EH25 9PS, UK
D. Nicholson
Affiliation:
Roslin Institute, Roslin, Midlothian, EH25 9PS, UK
A. Barr
Affiliation:
University of Bristol, Division of Companion Animal Studies, Langford, Bristol, BS40 5DU, UK
C.S. Haley*
Affiliation:
Roslin Institute, Roslin, Midlothian, EH25 9PS, UK
*
†To whom correspondence should be addressed. E-mail: [email protected]
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Abstract

Data from the F2 generation of a Large White (LW) ✕ Meishan (MS) crossbred population were analysed to detect quantitative trait loci (QTL) for leg and gait scores, osteochondrosis and physis scores. Legs, feet and gait score were assessed in 308 F2 animals at 85 ( + 5) kg and osteochondrosis and physis scores were recorded for the right foreleg after slaughter. A genome scan was performed using 111 genetic markers chosen to span the genome that were genotyped on the F2 animals and their F1 parents and purebred grandparents. A QTL on chromosome 1 affecting gait score was significant at the genome-wide significance level. Additional QTL significant at the chromosome-wide 5% threshold level (approx. equivalent to the genome-wide suggestive level) were detected on chromosome 1 for front feet and back legs scores, on chromosome 13 for front legs and front feet scores, on chromosome 14 for front legs, front feet and back legs scores and on chromosome 15 for back feet score. None of the QTL for osteochondrosis score exceeded the chromosome-wide suggestive level, but one chromosome-wide QTL for physis score was found on chromosome 7. On chromosome 1, gait and front feet scores mapped to the middle of the chromosome and showed additive effects in favour of the LW alleles and no dominance effects. The QTL for back legs score mapped to the distal end of the chromosome and showed a dominant effect and no additive effect. On chromosomes 14 and 15, the LW allele was again superior to the MS allele. On chromosome 13, there were both additive and dominance effects in favour of the MS allele. The MS alleles on chromosome 13 may have potential for introgression into a commercial LW population. The other putative QTLs identified may have value in marker-assisted selection in LW or MS-synthetic populations.

Keywords

genetic markersleg weaknessosteochondrosisquantitative trait locipigs.
Type
Breeding and genetics
Information
Animal Science , Volume 76 , Issue 2 , April 2003 , pp. 155 - 165
Copyright
Copyright © British Society of Animal Science 2003

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