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Changes in adipose tissue accumulation in Rasa Aragonesa breed lambs during growth and fattening

Published online by Cambridge University Press:  02 September 2010

A. Arana ,
B. Soret ,
J. A. Mendizabal ,
M. Corroza ,
P. Eguinoa  and
A. Purroy
A. Arana
Affiliation:
ETSIA, Universidad Pública de Navarra, Campus de Arrosadía, 31006 Pamplona, Spain
B. Soret
Affiliation:
ETSIA, Universidad Pública de Navarra, Campus de Arrosadía, 31006 Pamplona, Spain
J. A. Mendizabal
Affiliation:
ETSIA, Universidad Pública de Navarra, Campus de Arrosadía, 31006 Pamplona, Spain
M. Corroza
Affiliation:
ETSIA, Universidad Pública de Navarra, Campus de Arrosadía, 31006 Pamplona, Spain
P. Eguinoa
Affiliation:
ETSIA, Universidad Pública de Navarra, Campus de Arrosadía, 31006 Pamplona, Spain
A. Purroy
Affiliation:
ETSIA, Universidad Pública de Navarra, Campus de Arrosadía, 31006 Pamplona, Spain
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Abstract

Changes during growth and fattening in the number and size of adipocytes and in the activity of the lipogenic enzymes glycerol 3-phosphate dehydrogenase (G3PDH), fatty acid synthetase (FAS) and lipoprotein lipase (LPL) were studied in perirenal (PR) and subcutaneous (SO adipose depots of 28 male lambs of Rasa Aragonesa Spanish breed. Three groups of animals were slaughtered at: 32 (s.d. 6) (no. = 10), 89 (s.d. 8) (no. = 10) and 120 (s.d. 8) (no. = 8) days of age. A significant increase in the quantity of fat was observed as the age of the lambs increased (P < 0·001). Fat deposition was higher between 89 and 120 days of age. The number of adipocytes in the PR depot did not change but hypertrophy in this depot continued during the whole period studied (P < 0·001). In the SC depot a significant increase in adipocyte volume was only found between 89 and 120 days of age (P < 0·001). The increase in G3PDH activity (which estimates fatty acid esterification) and FAS activity (which estimates fatty acid synthesis) was greater in the final phase of the study. Besides, LPL enzyme activity (which estimates the uptake of plasma fatty acids) increased between 32 and 89 days of age in both depots and between 89 and 120 days in PR depot (P < 0·001).

Keywords

adipocytesadipose tissueenzyme activitylambslipogenesis
Type
Research Article
Information
Animal Science , Volume 66 , Issue 2 , April 1998 , pp. 409 - 413
Copyright
Copyright © British Society of Animal Science 1998

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References

Bas, P. 1992. Changes in activities of lipogenic enzymes in adipose tissue and liver of growing goats Journal of Animal Science 70: 3857–3866.Google Scholar
Belfrage, P. and Vaughan, M. J. 1969. Sample liquid-liquid partition system for isolation of labelled oleic acid from mixtures with glycerole Lipid Research 10: 341–344.Google Scholar
Biocom. 1992. Photometric image analysis system. BP53–91942 Les Ulis, Cedex, France, 219 pp.Google Scholar
Cryer, A. and Jones, H. M. 1978. Changes in the lipoprotein lipase (clearing factor lipase) activity of white adipose tissue during development of the rat Biochemical Journal 172: 319–325.Google Scholar
Etherton, T. D., Thompson, E. H. and Allen, C. E. 1977. Improved techniques for studies of adipocyte cellularity and metabolism Journal of Lipid Research 18: 552–557.Google Scholar
Halestrap, A. P. and Denton, R. M. 1973. Insulin and the regulation of adipose tissue acetyl coenzymeA carboxilase. Biochemical Journal 105:529–536.Google Scholar
Harvey, W. R. 1984. User's guide for LSMLMW PC-1 version Ohio State University Press, Columbus, Ohio, USA. 59pp.Google Scholar
Haugebak, C. D., Hedrick, H. B. and Asplund, J. M. 1974. Adipose tissue accumulation and cellularity in growing and fattening lambs. Journal of Animal Science 39: 1016–1025.Google Scholar
Hirsch, J. and Gallian, E. 1968. Methods for the determination of adipose cell size in man and animals. Journal of Lipid Research 9:110–119.Google Scholar
Hocquette, J. F. and Olivecrona, T. 1995. Variations in lipoprotein-lipase activity in muscle and adipose tissues between preruminant and weaned calves. Annales de Zootechnie 44: (supplement) 294.Google Scholar
Hood, R. L. and Thornton, R. F. 1980. The effect of compensatory growth on lipogenesis in ovine carcass adipose tissue. Australian Journal of Agricultural Research 155–161.CrossRefGoogle Scholar
Nougues, J. and Vezinhet, A. 1977. Evolution, pendant la croissance, de la cellularite du tissu adipeux chez le lapin et l'agneau. Annales de Biologie Animate, Biochimie et Biophysique 17: 799–806.CrossRefGoogle Scholar
Purroy, A., Mendizabal, J. A., Soret, B., Arana, A. and Mendizabal, F. J., 1997. Changes in cell number and size and in lipogenic enzyme activity in adipose tissues during growth and fattening of Lacha (Manech) lambs Annales de Zootechnie 46: 309–313.Google Scholar
Vernon, R. G. 1980. Lipid metabolism in the adipose tissue of ruminant animals Progress in Lipid Research 19:23–106.Google Scholar
Vernon, R. G. and Clegg, R. A. 1985. The metabolism of white adipose tissue in vivo and in vitro. In New perspectives in adipose tissue, structure, function and development (ed. , Cryer and Van, R. I. R.), pp. 65–86. Butterworths, London.Google Scholar
Vézinhet, A. and Nouguès, J. 1977. Evolution postnatale de la lipogenèse dans le tissu adipeux et le foie du mouton et du lapin. Annales de Biologie Animale, Biochimie et 17: 851–863.CrossRefGoogle Scholar
Wisse, L. S. and Green, H. 1979. Participation of one isozyme of cytosolic glycerophosphate dehydrogenase in the adipose conversion of 3T3 cells Journal of Biological Chemistry 254: 273–275.Google Scholar