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Variability of soya-bean lectin binding sites in the brush border membranes of calf intestinal epithelium

Published online by Cambridge University Press:  02 September 2010

H. G. C. J. M. Hendriks ,
J. F. J. G. Koninkx ,
M. Draaijer ,
J. E. van Dijk ,
J. A. M. Raaijmakers  and
J. M. V. M. Mouwen
H. G. C. J. M. Hendriks
Affiliation:
Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 1, 3584 CL Utrecht, The Netherlands
J. F. J. G. Koninkx
Affiliation:
Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 1, 3584 CL Utrecht, The Netherlands
M. Draaijer
Affiliation:
Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 1, 3584 CL Utrecht, The Netherlands
J. E. van Dijk
Affiliation:
Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 1, 3584 CL Utrecht, The Netherlands
J. A. M. Raaijmakers
Affiliation:
Department of Pulmonary Diseases, University Hospital, Catharijnesingel 101, 3511 GV Utrecht, The Netherlands
J. M. V. M. Mouwen
Affiliation:
Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 1, 3584 CL Utrecht, The Netherlands
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Abstract

An enzyme-linked lectin sorbent assay (ELLSA) has been used to determine quantitatively the amount of soya-bean agglutinin (SBA) binding sites in and the sialic acid contents of small intestinal brush border membranes (BBM) of a group of 14 clinically healthy veal calves. Computer analysis of the results indicated 704 (s.e. 27) nmol SBA binding sites per mg of BBM protein with a Kj of 2·63 (s.e. 0·15) × 10-5mol/l; the sialic acid contents were 2·67 (s.e. 0·43) mg/g BBM protein. We could not demonstrate a correlation between the amount of binding sites and the sialic acid contents of the BBM.

Incubation with neuraminidase did not alter the number of SBA binding sites.

Type
Papers
Information
Animal Production , Volume 49 , Issue 2 , October 1989 , pp. 229 - 232
Copyright
Copyright © British Society of Animal Science 1989

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References

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