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Oral administration of spermine advances intestinal maturation in sucking piglets

Published online by Cambridge University Press:  09 March 2007

Z. B. Cheng ,
D. F. Li ,
J. J. Xing ,
X. Y. Guo  and
Z. J. Li
Z. B. Cheng
Affiliation:
National Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100094, People's Republic of China Department of Animal Science and Technology, Yunnan Agricultural University, Kunming City, Yunnan Province 650201, People's Republic of China
D. F. Li*
Affiliation:
National Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100094, People's Republic of China
J. J. Xing
Affiliation:
National Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100094, People's Republic of China
X. Y. Guo
Affiliation:
National Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100094, People's Republic of China
Z. J. Li
Affiliation:
National Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100094, People's Republic of China
*
†Corresponding author: E-mail: [email protected]
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Abstract

The objective of this study was to investigate the effect of orally administered spermine at various doses on intestinal maturation in sucking piglets. Thirty-six 11-day-old sucking piglets were assigned randomly to one of six treatments to receive via a stomach tube 0, 0·1, 0·2, 0·3, 0·4, or 0·5 mmol spermine per kg live weight (LW) per day for 3 days. At day 14 of age, duodenum, jejunum and ileum were obtained for biochemical and morphological analysis. Increasing the dose of orally administered spermine increased intestinal weight (linear effect, P<0·01), mucosal weight (linear effect, P<0·05), and mucosal protein, DNA and RNA contents of the duodenum (linear effect, P≤0·01) and jejunum (linear effect, P<0·01). Elevating spermine doses also enhanced (linear effect, P≤0·02) the specific activities of maltase and sucrase but decreased (linear effect, P<0·01) lactase specific activity in the jejunum and duodenum. Furthermore, augmenting oral doses of spermine increased crypt depth and villus width but reduced villus height in the jejunum (linear effect, P<0·05) and duodenum (linear effect, P<0·01). For most measurements, the effects were observed at the oral spermine doses of 0·3 to 0·5 mmol/kg LW per day. Collectively, the results show that oral administration of optimal doses of spermine to 11-day-old sucking piglets induces precocious intestinal maturation and promotes intestinal growth.

Keywords

disaccharidasesintestinespigletsspermine
Type
Research Article
Information
Animal Science , Volume 82 , Issue 5 , October 2006 , pp. 621 - 626
Copyright
Copyright © British Society of Animal Science 2006

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References

Bardocz, S., Duguid, T. J., Brown, D. S., Grant, G., Pusztai, A., White, A. and Ralph, A. 1995. The importance of dietary polyamines in cell regeneration and growth. British Journal of Nutrition 73: 819–828.Google Scholar
Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248–254.CrossRefGoogle ScholarPubMed
Buts, J. P., De Keyser, N., Kolanowski, J., Sokal, E. and Van Hoof, F. 1993. Maturation of villus and crypt cell functions in rat small intestine: Role of dietary polyamines. Digestive Diseases and Science 38: 1091–1098.CrossRefGoogle ScholarPubMed
Cranwell, P. D. 1995. Development of the neonatal gut and enzyme systems. In The neonatal pig: development and survival (ed. Varley, M. A.), pp. 99–154, CAB International, Wallingford, UK.Google Scholar
Cranwell, P. D. and Moughan, P. J. 1989. Biological limitations imposed by the digestive system to the growth performance of weaned pigs. In Manipulating pig production II (ed. Barnett, J. L. and Hennessy, D. P.). pp. 140–183. Australasian Pig Science Association, Attwood, Victoria.Google Scholar
Crea, K. R., Mahan, D. C., Cross, R. F., Reinhart, G. A. and Whitmoyer, R. E. 1988. Effect of age, weaning and postweaning diet on small intestinal growth and jejunal morphology in young swine. Journal of Animal Science 66: 574–584.Google Scholar
Dahlqvist, A. 1964. Method for assay of intestinal disaccharidase. Analytical. Biochemistry 7: 18–25.Google Scholar
Dufour, C., Dandrifosse, G., Forget, P., Vermesse, F., Romain, N. and Lepoint, P. 1988. Spermine and spermidine induce intestinal maturation in the rat. Gastroenterology 95: 112–116.CrossRefGoogle ScholarPubMed
Ewtushik, A. L., Bertolo, R. F. P. and Ball, R. O. 2000. Intestinal development of early-weaned piglets receiving diets supplemented with selected amino acids or polyamines. Canadian Journal of Animal Science 80: 653–662.Google Scholar
Fan, M. Z. 2003. Growth and ontogeny of the gastrointestinal tract. In The neonatal pig: gastrointestinal physiology and nutrition (ed. Xu, R. J., Cranwell, P.), pp. 31–60. Nottingham University Press, Nottingham.Google Scholar
Grant, A. L., Thomas, J. W., King, K. J. and Liesman, J. S. 1990. Effects of dietary amines on small intestinal variables in neonatal pigs fed soy protein isolate. Journal of Animal Science 68: 363–371.Google Scholar
Hampson, D. J. 1986. Alterations in piglet small intestinal structure at weaning. Research in Veterinary Science 40: 32–40.Google Scholar
Hampson, D. J. and Kidder, D. E. 1986. Influence of creep feeding and weaning on brush border enzyme activities in the piglet small intestine. Research in Veterinary Science 40: 24–31.Google Scholar
Henning, S. J. 1985. Ontogeny of enzymes in the small intestine. Annual Review of Physiology 47: 231–245.Google Scholar
Jin, L., Reynolds, P., Redmer, D. A., Caton, J. S. and Crenshaw, J. D. 1994. Effects of dietary fiber on intestinal growth, cell proliferation, and morphology in growing pigs. Journal of Animal Science 72: 2270–2278.Google Scholar
Johnson, L. R., Tseng, C. C., Wang, P., Tipnis, U. R. and Haddox, M. K. 1989. Mucosal ornithine decarboxylase in the small intestinal: localization and stimulation. American Journal of Physiology: Gastrointestinal and Liver Physiology 256: G624–G630.Google Scholar
Kaouass, M., Deloyer, P., Romain, P. and Dandrifosse, G. 1994. Spermine-induced precocious intestinal maturation in suckling rats. Digestive Diseases and Sciences 41: 1434–1444.Google Scholar
Li, Z. T., Li, D. F., Qiao, S. Y., Zhu, X. P. and Huang, C. H. 2003. Anti-nutritional effects of a moderate dose of soybean agglutinin in the rat. Archives of Animal Nutrition 57: 267–277.Google Scholar
Loret, S., Brolet, P., Pierzymowski, S., Gouders, I., Klimek, M., Danielson, V., Rosted, A., Lesniewska, V. and Dandrifosse, G. 2000. Pancreatic exocrine secretions as a source of luminal polyamines in pigs. Experimental Physiology 85: 301–308.Google Scholar
Miller, B. G., James, P. S., Smith, M. W. and Bourne, F. J. 1986. Effect of weaning on the capacity of pig intestinal villi to digest and absorb nutrients. Journal of Agricultural Science 107: 579–589.Google Scholar
Morisset, J. 1992. Regulation of growth and development of the gastrointestinal tract. Journal of Dairy Science 76: 2080–2093.Google Scholar
Peulen, O., Deloyer, P. and Dandrifosse, G. 2004. Short-term effects of spermine ingestion on the small intestine: a comparison of suckling and weaned rats. Reproduction, Nutrition, Development 44: 353–364.Google Scholar
Peulen, O., Deloyer, P., Grandfis, C., Loret, S. and Dandrifosse, G. 2000. Intestinal maturation induced by spermine in young animal. Livestock Production Science 66: 109–120.CrossRefGoogle Scholar
Peulen, O., Pirlet, C., Klimek, M., Goffinet, G. and Dandrifosse, G. 1998. Comparison between the natural postnatal maturation and the spermine-induced maturation of the rat intestine. Archives of Physiology and Biochemistry 106: 46–55.Google Scholar
Reynolds, L. P., Ferrell, C. L., Nienaber, J. A. and Ford, S. P. 1985. Effect of chronic environmental heat stress on blood flow and nutrient uptake of the gravid bovine uterus and foetus. Journal of Agricultural Science 104: 289–297.CrossRefGoogle Scholar
Statistical Analysis Systems Institute. 1989. SAS/STAT user's guide, version 6. SAS Institute Inc., Cary, NC.Google Scholar
Shimizu, K., Mushiake, S., Yoshimura, N., Harada, T. and Okada, S. 1993. The effect of spermine on the disaccharidase activities in suckling rats of different age. Cell Biology International 17: 543–546.CrossRefGoogle ScholarPubMed
Tabor, C. W. and Tabor, H. 1984. Polyamines. Annual Review of Biochemistry 53: 749–790.Google Scholar
Ter Steege, J. C., Buurman, W. A. and Forget, P. P. 1997. Spermine induces maturation of the immature intestinal immune system in neonatal mice. Journal of Pediatric Gastroenterology and Nutrition 25: 332–340.Google Scholar
United States Department of Human Health and Services. 1996. Guide for the care and use of laboratory animals. National Institute of Health, Washington, DC.Google Scholar
Wu, G., Flynn, N. E. and Knabe, D. A. 2000a. Enhanced intestinal synthesis of polyamines from proline in cortisol-treated piglets. American Journal of Physiology: Endocrinology and Metabolism 279: E395–E402.Google Scholar
Wu, G., Flynn, N. E., Knabe, D. A. and Jaeger, L. A. 2000b. A cortisol surge mediates the enhanced polyamine synthesis in porcine enterocytes during weaning. American Journal of Physiology: Regulatory, Integrative and Comparative Physiology 279: R554–R559.Google Scholar
Wu, G., Knabe, D. A., Flynn, N. E., Yan, W. and Flynn, S. P. 1996a. Arginine degradation in developing porcine enterocytes. American Journal of Physiology: Gastrointestinal and Liver Physiology 271: G913–G919.Google Scholar
Wu, G., Meier, S. E. and Knabe, D. A. 1996b. Dietary glutamine supplementation prevents jejunal atrophy in weaned pigs. Journal of Nutrition 126: 2578–2584.Google Scholar