Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-24T14:32:33.489Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of pyridoxine supplementation on dietary protein utilization in gilthead seabream fry

Published online by Cambridge University Press:  02 September 2010

R. T. M. Baker  and
S. J. Davies
R. T. M. Baker
Affiliation:
Fish Nutrition Unit, Department of Biological Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA
S. J. Davies
Affiliation:
Fish Nutrition Unit, Department of Biological Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA
Get access

Abstract

A study was undertaken to ascertain the effect of pyridoxine in diets of varying protein to energy ratio (P: E) for Mediterranean gilthead seabream fry (Sparus aurata) with respect to protein utilization. Diets were formulated to provide three levels of added pyridoxine (0·5, 5·0 and 50·0 mg/kg), at two protein levels (320 or 470 g/kg) whilst maintaining a similar gross isocalorific profile at 20 MJ/kg. Feeding level was proportionately 0·02 of the fish body weight per day, resulting in the following effects after a 70-day feeding trial. Low P: E ratio diets (15 g/MJ) did not perform as well as high P: E diets (24 g/MJ), with respect to growth related indices. Higher inclusions of pyridoxine into diets of equal P: E ratios, caused elevations in food conversion efficiency, protein efficiency ratio and apparent net protein utilization. A marked improvement of growth rate concomitant with pyridoxine level only occurred in the high P: E diets. It was concluded that incremental additions of pyridoxine in the diet, increased the efficiency of protein utilization for all diets tested. However, the protein sparing effect oflipid was not achieved for the low protein diets indicating the relatively high requirement for protein in the growth of the juvenile seabream.

Keywords

conversiongrowthprotein utilizationpyridoxineseabream
Type
Research Article
Information
Animal Science , Volume 60 , Issue 1 , February 1995 , pp. 157 - 162
Copyright
Copyright © British Society of Animal Science 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Adron, J. W., Knox, D., Cowey, C. B. and Ball, G. T. 1978. Studies on the nutrition of marine flatfish; the pyridoxine requirement of turbot, Scophthalmus maximus. British journal of Nutrition 40: 261–268.Google Scholar
Association of Official Analytical Chemists. 1990. Official methods of analysis, 15th edition (ed. Helrich, K.). Association of Official Analytical Chemists, Washington, DC.Google Scholar
Andrews, J. W. and Murai, T. 1979. Pyridoxine requirements for channel catfish. Journal of Nutrition 109: 533–537.Google Scholar
Beamish, F. W. H. and Medland, T. E. 1986. Protein sparing effects in large rainbow trout (Salmo gairdneri). Aquaculture 55: 35–42.CrossRefGoogle Scholar
Casillas, E., Sundquist, J. and Ames, W. E. 1982. Optimization of assay conditions for, and the selected tissue distribution of, alanine aminotransferase and aspartate aminotransferase of English sole, Parophrys vetulus Girard. Journal of Fish Biology 21: 197–204.Google Scholar
Castell, J. D. and Olivier, G. 1986. The importance of nutrition in disease resistance and immune responses of fish. Proceedings of the third international conference on aquafarming — aquacoutoura 86, Verona, Italy (ed. Grimaldi, E. and Rosenthal, H.).Google Scholar
Cho, C. Y., Cowey, C. B. and Watanabe, T. 1983. Finfish nutrition in Asia: methodological approaches to research and development. IDRC, Ottawa.Google Scholar
Clark, A. E., Watanabe, W. O., Olla, B. L. and Wicklund, R. I. 1990. Growth, feed conversion and protein utilisation of Florida red tilapia fed isocaloric diets with different protein levels in seawater pools. Aquaculture 88: 75–85.CrossRefGoogle Scholar
D'Apollonia, S. and Anderson, P. D. 1980. Optimal assay conditions for serum and liver glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, and sorbitol dehydrogenase from the rainbow trout, salmo gairdneri. Canadian Journal of Fisheries and Aquatic Science 37: 163–169.Google Scholar
Davies, S. J., McConnell, S. and Bateson, R. I. 1990. Potential of rapeseed meal as an alternative protein source in complete diets for tilapia (Oreochromis mossambicus Peters). Aquaculture 87: 145–154.Google Scholar
Fernandez-Pato, C. A., Martinez-Tapia, C., Chereguini, O. and Sola, E. 1990. Use of pyridoxine and ascorbic acid at different doses in turbot (Scophthalmus maximus) in experimental feeding. ICES, Copenhagen, Denmark.Google Scholar
Gropp, J. 1979. Standard fish husbandry designs of experiments and their evaluation. In Finfish nutrition and fishfeed technology, volume II. Proceedings of a world symposium on finfish nutrition and fishfeed technology, Hamburg, 1978 (ed. Halver, J. E. and Tiews, K.).Google Scholar
Halver, J. E. 1989. Fish nutrition. 2nd ed. Academic Press, London.Google Scholar
Hardy, R. W., Casillas, E. and Masumoto, T. 1987. Determination of vitamin B6 deficiency in rainbow trout (Salmo gairdneri) by liver enzyme assay and HPLC analysis. Canadian Journal of Fisheries and Aquatic Science 44: 219–222.Google Scholar
Herman, R. L. 1985. Histopatology associated with pyridoxine deficiency in Atlantic salmon (Salmo salar). Aquaculture 46: 173–177.Google Scholar
Jurss, K. 1978. The effect of pyridoxine deficiency on aminotransferase activity in liver and white-muscle of rainbow trout (Salmo gairdneri). Comparative Biochemistry and Physiology 61B: 385–389.Google Scholar
Kissil, G. W., Cowey, C. B., Adron, J. W. and Richards, R. H. 1981. Pyridoxine requirements of the gilthead bream, Sparus aurata. Aquaculture 23: 243–255.Google Scholar
Leith, D., Holmes, J., Kaattari, S., Yui, M. and Jones, T. 1985. Effects of vitamin nutrition on the immune response of hatchery reared salmonids. Annual report, 1984, Oregon State University, Corvallis (USA), Department of Microbiology.Google Scholar
Martin, J. M. V. 1992. Studies on the utilization of dietary protein and energy by gilthead seabream (Sparus aurata L.). Ph.D. thesis, University of Stirling.Google Scholar
McDonald, P., Edwards, R. A. and Greenhalgh, J. F. D. 1990. Animal nutrition. Longman Scientific and Technical, Harlow.Google Scholar
McGilvery, R. W. and Goldstein, G. W. 1983. Biochemistry: a functional approach. 3rd ed. W. B. Saunders Company, London.Google Scholar
Phillips, A. M., Podoliak, H. A., Livingston, D. L., Dumas, R. F. and Thoesen, R. W. 1960. Effect of dietary protein on the sensitivity of brook trout to a pyridoxine deficiency. Fisheries Research Bulletin 23: 46–55.Google Scholar
Pike, R. L. and Brown, M. L. 1984. Nutrition: an integrated approach. 3rd ed. John Wiley, New York.Google Scholar
Slinger, S. J., Razzaque, A. and Cho, C. Y. 1979. Effect of feed processing and leaching on the losses of certain vitamins in fish diets. In Finfish nutrition and fishfeed technology, volume II. Proceedings of a world symposium on finfish nutrition and fishfeed technology, Hamburg, 1978 (ed. Halver, J. E. and Tiews, K.).Google Scholar
Steel, R. G. D. and Torrie, J. H. 1960. Principles and procedures of statistics: a biomctric approach. 2nd ed. McGraw Hill, New York.Google Scholar
Steffens, W. 1989. Principles of fish nutrition. Ellis Harwood, Chichester.Google Scholar
Stryer, L. 1988. Biochemistry. 3rd ed. W. H. Freeman, New York.Google Scholar
Takeda, T. and Yone, Y. 1971. Studies of nutrition of red sea bream. 2. Comparison of vitamin B6 requirement level between fish fed a synthetic diet and fish fed beef liver during the prefeeding period. Report of the Fisheries Research Laboratory, Kyushu University 1: 37–47.Google Scholar
Watanabe, T., Takeuchi, T. and Ogino, C. H. 1979. Studies on the sparing effects of lipids on dietary protein in rainbow trout (Salmo gairdneri). In Finfish nutrition and fishfeed technology, volume I. Proceedings of a world symposium on finfish nutrition and fishfeed technology, Hamburg, 1978 (ed. Halver, J. E. and Tiews, K.), pp. 593.Google Scholar
Wanakowat, J., Boonyaratpalin, M., Pimoljinda, T. and Assavaaree, M. 1989. Vitamin B6 requirement of juvenile Seabass, Lates calcarifer. In The current status of fish nutrition in aquaculture. Proceedings of the third international symposium on feeding and nutrition in fish, Toba, Japan.Google Scholar