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Effect of ammoniation or protein supplementation of barley straw on digestion and purine derivative excretion in sheep

Published online by Cambridge University Press:  02 September 2010

P. O. Mawuenyegah ,
L. Warly ,
T. Harumoto  and
T. Fujihara
P. O. Mawuenyegah
Affiliation:
Shimane University, Faculty of Agriculture, Matsue, Shimane 690, Japan
L. Warly
Affiliation:
Shimane University, Faculty of Agriculture, Matsue, Shimane 690, Japan
T. Harumoto
Affiliation:
Shimane University, Faculty of Agriculture, Matsue, Shimane 690, Japan
T. Fujihara
Affiliation:
Shimane University, Faculty of Agriculture, Matsue, Shimane 690, Japan
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Abstract

An experiment was conducted to examine the effect of ammonia treatment or soya-bean meal supplementation on rumen and lower tract digestion and urinary purine-derivative excretion in sheep given barley straw-based diets. Four wethers each fitted with simple rumen and duodenal cannulae were randomly allocated to four diets in a 4×4 Latin-square design. The diets were, untreated barley straw + molasses meal (diet 1), ammonia-treated barley straw + molasses meal (diet 2), untreated barley straw + soya-bean meal + molasses meal (diet 3) and ammonia-treated barley straw + soya-bean meal + molasses meal (diet 4). Dry-matter (DM) intake, total tract neutral- and acid-detergent fibre apparent digestibility and faecal nitrogen (N) output were higher for diets 2 and 4. Microbial protein yield, determined from total urinary purine-derivative excretion, was also high in sheep on diets 2 and 4. However, supplementation with soya-bean meal in diet 4 reduced DM and fibre digestion but increased intake and microbial synthesis. Despite the lower microbial protein synthesis for diet 3, total tract N digestion was high for the diet, an indication of higher nutrient absorption in the lower tract. The above results indicated that there was a substantial increase in rumen microbial N yield from ammoniated straw-based diets. However, not all the N was made available to the animals. Some of the microbial N absorbed in the lower tract may have been overestimated by the use of the purine derivative-microbial N evaluation method. On the other hand, when protein supplements were given the amount of microbial protein available to the ruminant through absorption in the lower tract may have been underestimated by urinary purine derivatives.

Keywords

ammonia treatmentprotein supplementspurinessheepstraw
Type
Research Article
Information
Animal Science , Volume 64 , Issue 3 , June 1997 , pp. 433 - 439
Copyright
Copyright © British Society of Animal Science 1997

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References

Abdulrazak, S. A., Chen, X. B. and Ørskov, E. R. 1992. The effect of supplementing ammonia-treated straw with sugar-beet pulp or barley on rumen kinetics and microbial protein production. Animal Production 54: 506 (abstr.).Google Scholar
Agricultural and Food Research Council. 1993. Energy and protein requirements of ruminants. AFRC Technical Committee on Responses to Nutrients. CAB International, Wallingford.Google Scholar
Association of Official Analytical Chemists. 1984. Official methods of analysis, 13th ed. Association of Official Analytical Chemists, Washington, DC.Google Scholar
Balcells, J., Fondevila, M., Guada, J. A., Castrillo, C. and Surra, J. C. E. 1993a. Urinary excretions of purine derivatives and nitrogen in sheep given straw supplemented with different sources of carbohydrates. Animal Production 57: 287–292.Google Scholar
Balcells, J., Guada, J. A., Castrillo, C. and Gasa, J. 1993b. Rumen digestion and urinary excretion of purine derivatives in response to urea supplementation of sodium-treated straw fed to sheep. British Journal of Nutrition 69: 721–732.CrossRefGoogle ScholarPubMed
Cann, I. K. O., Kobayashi, Y., Wakita, M. and Hoshino, S. 1991. Digestion properties in the rumen and lower tract of sheep. Animal Feed Science and Technology 35: 55–68.CrossRefGoogle Scholar
Chen, X. B., Chen, Y. K., Franklin, M. F., Ørskov, E. R. and Shand, W. J. 1992. The effect of feed intake and body weight on purine derivative excretion and microbial protein supply in sheep, Journal of Animal Science 70: 1534–1542.CrossRefGoogle ScholarPubMed
Chen, X. B., Hovell, F. D. deB., Ørskov, E. R. and Brown, D. S. 1990. Excretion of purine derivatives by ruminants: effect of exogenous nucleic acid supply on purine derivatives excretion by sheep. British Journal of Nutrition 63: 131–142.CrossRefGoogle ScholarPubMed
Church, D. C. and Santos, A. 1981. Effect of graded levels of soybean meal and non-protein nitrogen-molasses supplement on consumption and digestibility of wheat straw. Journal of Animal Science 53: 1609–1615.CrossRefGoogle Scholar
Duncan, D. B. 1955. Multiple range and multiple F tests. Biometrics 11: 1–42.CrossRefGoogle Scholar
Erwin, E. S., Marco, G. S. and Emery, E. M. 1961. Volatile fatty acid analyses of blood and rumen fluid by gas chromatography. Journal of Dairy Science 44: 1768–1774.CrossRefGoogle Scholar
Fahmy, S. T. M. and Ørskov, E. R. 1984. Digestion and utilization of straw. 1. Effect of different chemical treatments on degradability and digestibility of barley straw by sheep. Animal Production 38: 69–74.Google Scholar
Faichney, G. J. 1975. The use of markers to partition digestion within the gastro-intestinal tract of ruminants. In Digestion and metabolism in the ruminant (ed. McDonald, I. W. and Warner, A. C. I.), pp. 277–291. University of New England Publishing Unit, Armidale, NSW, Australia.Google Scholar
Fondevila, M., Castrillo, C., Guada, J. A. and Balcells, J. 1994. Effect of ammonia treatment and carbohydrate supplementation of barley straw on rumen liquid characteristics and substrate degradation by sheep. Animal Feed Science and Technology 50: 137–155.CrossRefGoogle Scholar
Fujihara, T., Ørskov, E. R. and Reeds, P. J. 1987. The effect of protein infusion on urinary excretion of purine derivatives in ruminants nourished by intragastric nutrition. Journal of Agricultural Science, Cambridge 109: 7–12.CrossRefGoogle Scholar
Goering, H. K. and Van Soest, P. J. 1970. Forage fibre analyses (apparatus, reagents, procedures and some applications). Agricultural handbook number 379, US Department of Agriculture, Washington, DC.Google Scholar
McAllan, A. B. and Smith, R. H. 1973. Degradation of nucleic acid derivatives by rumen bacteria in vitro. British Journal of Nutrition 29: 467–474.CrossRefGoogle ScholarPubMed
Mawuenyegah, O., Chiba, T., Harumoto, T. and Fujihara, T. 1994. Effect of ammonia treatment and soya-bean meal supplementation on the utilization of barley straw by sheep. Proceedings of the Society of Nutrition and Physiology 3: 26 (abstr.).Google Scholar
Morimoto, H. 1971. Dobutsu Eiyo Shiken Ho (Methods in examination of animal nutrition), pp. 395–396. Yokendo, Tokyo.Google Scholar
Oser, B. L. 1965. Hawke's physiological chemistry, 14th ed., 1219. Mcgraw-Hill Book Company, New York.Google Scholar
Puchala, R. and Kulasek, G. W. 1992. Estimation of microbial protein flow from the rumen of sheep using microbial nucleic acid and urinary excretion of purine derivatives. Canadian Journal ofAnimal Science 72: 821–830CrossRefGoogle Scholar
Satter, L. D. and Roffler, R. E. 1981. Influence of roughage and carbohydrate inputs on rumen fermentation. In Recent developments in ruminant nutrition (ed. Haresign, W. and Cole, D. J. A.), pp. 115–139. Butterworths, London.CrossRefGoogle Scholar
Smith, R. A. and McAllan, A. B. 1970. Nucleic acid metabolism in the ruminant. British Journal of Nutrition 24: 545–556.CrossRefGoogle ScholarPubMed
Statistical Analysis Systems Institute. 1985. SAS user's guide: statistics, fifth ed. SAS Institute Inc., Cary, NC.Google Scholar
Sundstol, F., Coxworth, E. and Mowat, D. N. 1978. Improving the nutritive value of straw and other low quality roughages by treatment with ammonia. World Animal Review 26: 13–21.Google Scholar
Topps, J. H. and Elliot, R. C. 1965. Relationship between concentration of ruminal nucleic acid and excretion of purine derivatives by sheep. Nature, London 205: 498–499.CrossRefGoogle Scholar
Van Soest, P. J., Mascarenhas Ferreira, A. and Hartley, R. D. 1984. Chemical properties of fibre in relation to nutritive quality of ammonia-treated forages. Animal Feed Science and Technology 10: 155–164. pp.CrossRefGoogle Scholar
Warly, L., Fariani, A., Mawuenyegah, O. P., Matsui, T., Fujihara, T. and Harumoto, T. 1994. Studies on the utilization of rice straw by sheep. III. Effect of soybean meal and barley supplementation on voluntary intake, digestibility and ruminal fermentation. Asian-Australasian Journal ofAnimal Sciences 7: 265–271.CrossRefGoogle Scholar
Young, E. G. and Conway, C. F. 1942. On the estimation of allantoin by the Rimini-Schryner reaction. Journal of Biological Chemistry 142: 839–853.CrossRefGoogle Scholar