Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-30T16:17:24.056Z Has data issue: false hasContentIssue false
  • English
  • Français

Diurnal variation of progesterone levels in milk and milk fat of crossbred cows during the oestrous cycle and early pregnancy

Published online by Cambridge University Press:  02 September 2010

S. K. Batra ,
G. S. Pahwa ,
A. K. Suri  and
R. S. Pandey
S. K. Batra
Affiliation:
Division of Dairy Chemistry, National Dairy Research Institute, Karnal-132001, India
G. S. Pahwa
Affiliation:
Division of Dairy Chemistry, National Dairy Research Institute, Karnal-132001, India
A. K. Suri
Affiliation:
Division of Dairy Chemistry, National Dairy Research Institute, Karnal-132001, India
R. S. Pandey
Affiliation:
Division of Dairy Chemistry, National Dairy Research Institute, Karnal-132001, India
Get access

Abstract

Changes in progesterone levels in milk and in milk fat were measured by radioimmunoassay in nine crossbred cows for 40 days after insemination. On average, progesterone in milk was minimal: 1·10μg/l at the morning milking (M) and 1·63μg/l at the evening milking (E) on the day of oestrus; this increased to 15·72 μg/l at M and 22·8 μg/1 at E on day 13 after insemination, and thereafter declined to 0·99 μg/l at M and 1·12μg/l at E on the day that the next oestrous cycle commenced in non-pregnant cows. In pregnant animals the level was maintained, and fluctuated from 1505 to 19·60μg/l at M and 18·09 to 23·01 μ/1 at E up to 40 days of pregnancy.

The average progesterone concentration in milk fat was 18·9μg/l at M and 24·1 μl at E on the day of oestrus, and rose to a peak of 141·7μ/l at M and 153·8μg/1 at E on day 13 after insemination. It continued to increase and fluctuated between 160·6 and 195·8μg/l at M, and 161·1 and 225·6μg/l at E, in pregnant animals, but dropped to 12·0μg/1 at M and 16·7 μg/l at E on the day of the succeeding oestrus in those animals which failed to conceive. Progesterone concentration in milk and in milk fat was significantly higher in evening samples than in morning samples (P<0·05).

Type
Research Article
Information
Animal Production , Volume 31 , Issue 2 , October 1980 , pp. 127 - 131
Copyright
Copyright © British Society of Animal Science 1980

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Arora, R. C., Bachlaus, N. K., Prasad, A. and Pandey, R. S. 1979. Plasma progesterone level and early pregnancy diagnosis in buffalo heifers. Indian J. exp. Biol. 17: 124–127.Google ScholarPubMed
Batra, S. K., Arora, R. C., Bachlaus, N. K. and Pandey, R. S. 1979. Blood and milk progesterone in pregnant and nonpregnant buffalo. J. Dairy Sci. 62: 1390–1393.CrossRefGoogle ScholarPubMed
Darling, J. A. B., Laing, A. H. and Harkness, R. A. 1974. A survey of the steroids in cows' milk. J. Endocr. 62: 291–297.CrossRefGoogle ScholarPubMed
Folman, Y., Rosenberg, M., Herz, Z. and Davidson, M. 1973. The relationship between plasma progesterone concentration and conception in post-partum dairy cows maintained on two levels of nutrition. J. Reprod. Fert. 34: 267–278.CrossRefGoogle ScholarPubMed
Hamburger, R. 1974. Progesteronebestimmung in der milch. Diss. med. vet., Univ. Munchen.Google Scholar
Heap, R. B., Gwyn, M., Laing, J. A. and Walters, D. E. 1973. Pregnancy diagnosis in cows; changes in milk progesterone concentration during the oestrous cycle and pregnancy measured by a rapid radioimmunoassay. J. agrk. Sci., Camb. 81: 151–157.CrossRefGoogle Scholar
Heap, R. B., Henville, A. and Linzell, J. L. 1975. Metabolic clearance rate, production rate, and mammary uptake and metabolism of progesterone in cows. J. Endocr. 66: 239–247.CrossRefGoogle ScholarPubMed
Hoffmann, B., Hamburger, R. and Hollwich, W. 1977. Determination of progesterone directly in milk fat as an improved method for fertility control in cattle. Zuchthygiene 12: 1–7.CrossRefGoogle ScholarPubMed
Laing, J. A. and Heap, R. B. 1971. The concentration of progesterone in the milk of cows during the reproductive cycle. Br. vet. J. 127: xix–xxii.CrossRefGoogle ScholarPubMed
McCracken, J. A. 1964. Progesterone in the body fat of the dairy cow. J. Endocr. 28: 339–340.CrossRefGoogle ScholarPubMed
Nuti, L. C., Wentworth, B. C., Karavolas, H. J., Tyler, W. J. and Ginther, O. J. 1975. Comparison of radio-immunoassay and gas-liquid chromatography analysis of progesterone concentrations in cow's milk. Proc. Soc. exp. Biol. Med. 139: 877–880.CrossRefGoogle Scholar
Pope, G. S., Majzlik, I., Ball, P. J. H. and Leaver, J. D. 1976. Use of progesterone concentrations in plasma and milk in the diagnosis of pregnancy in domestic cattle. Br. vet. J. 132: 497–506.CrossRefGoogle ScholarPubMed
Sebastian, J., Doss, K. D. V. V. and Rao, R. V. 1977. Determination of range of fat and SNF contents of milks of cattle in the region. Rep. natn. Dairy Res. lnst. Karnal p. 297.Google Scholar
Thibier, M., Fourbet, J. F. and Parez, M. 1976. Relationship between milk progesterone concentration and milk yield, fat and total nitrogen content. Br. vet. J. 132: 477–486.CrossRefGoogle ScholarPubMed