Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-28T05:05:20.864Z Has data issue: false hasContentIssue false
  • English
  • Français

Causes of differences in urinary excretion of purine derivatives in buffaloes and cattle

Published online by Cambridge University Press:  09 March 2007

Vo Thi Kim Thanh  and
E. R. Ørskov
Vo Thi Kim Thanh
Affiliation:
Hue University of Agriculture and Forestry, 102 Phung Hung, Hue, Vietnam
E. R. Ørskov*
Affiliation:
Macaulay Institute, Craigiebuckler, Aberdeen, AB15 8QH, UK
*
†Corresponding author. E-mail: [email protected]
Get access

Abstract

In experiment 1, three male calves of Vietnamese cattle and three maleVietnamese swamp buffalo calves were weaned after receiving colostrum and reared by bottle feeding of milk. During the 1st month the animal did not have access to solid food. Urine was collected to determine differences in endogenous excretion of purine between the two types of animal. After that they were given access to equal amount of solid food for 2 months to stimulate rumen development, urine was again collected to determine the differences in purine excretion. In experiment 2, the same animals were given milk mixed with purines in three treatments (0, 1·7 and 3·4 g/day). The same animals were used in experiment 3 for intravenous allantoin infusion, to test the effect of purines themselves introduced into the plasma.The results showed that in period 1 of experiment 1 there was no significant difference in purine excretion between the two types of animal. The excretion being 0·65 mmol/kg M 0·75 for cattle and 0·69 mmol/kg M 0·75for buffaloes calves, respectively. For period 2, after rumen development there were significant differences between two types of animal. The excretion from buffaloes (0·26 mmol/kg M0·75) being less than half that of cattle (0·69 mmol/kg M 0·75).In experiment 2 the regression of purine excretion mmol/day (y) was y=0·6279x+9·1496 for cattle calves and y=0·2618x+5·8594 for buffalo calves where x was the purine given.In experiment 3, from each mmol of allantoin infusion, the recovery was about 0·70 in cattle but only half (0·32) in buffaloes ( P<0·01).It is clearly shown that the difference in purine derivative excretion occur only after rumen development It is suggested that glomerular filtration rate may be lower in buffaloes than cattle leaving more time in the blood thus more time for recycling to the rumen and metabolized by bacteria or the permeability from the blood to the rumen is greater in buffaloes than cattle.

Keywords

buffaloescattlepurinesrumen
Type
Research Article
Information
Animal Science , Volume 82 , Issue 3 , June 2006 , pp. 355 - 358
Copyright
Copyright © British Society of Animal Science 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Chen, X. B. 1989. Excretion of purine derivatives by sheep and cattle and it use for the estimation of absorbed microbial protein. Ph.D. thesis, University of Aberdeen.Google Scholar
Chen, X. B., Fujihara, T., Nakamura, K., Mawuenyegah, P. O., Franklin, M. F. and Kyle, D. J. 1997. Response of urinary and plasma purine derivatives to various rates and infusion patterns of purines in sheep nourished by intragastric infusion. Journal of Agricultural Science, Cambridge 129: 343–352.CrossRefGoogle Scholar
Chen, X. B. and Oslash;rskov, E. R. 2004. Research on urinary excretion of purine derivatives in ruminants: past, present and future. In Estimation of microbial protein supply in ruminants using urinary purine derivative (ed. MakkarH. P. S., H. P. S., and Chen, X. B.), pp.180–210. FAO/IAEA, Kluwer Academic Publishers. Dordrecht.CrossRefGoogle Scholar
International Atomic Energy Agency 1997. Estimation of rumen microbial protein production from purine derivative in urine. In A laboratory manual for FAO/IAEA co-ordinated Research Program, IAEA-TECHDOC-945. IAEA, Vienna.Google Scholar
Liang, J. B., Pimpa, O., Abdullah, N. and Jeland, Z. A. 1999. Estimation of rumen microbial protein production from urinary purine derivatives in zebu cattle and water buffalo. In Nuclear based technologies for estimating microbial protein supply in ruminant livestock IAEA-TECDOC-1093, pp. 35–42IAEA, Vienna.Google Scholar
Liang, J. B., Pimpa, O., Balcells, J., Abdullah, N. and Jeland, Z. 2004. An overview on the use of urinary purine derivatives excretion as a method for estimation of rumen microbial protein production in swamp buffaloes and zebu cattle. In Estimation of microbial protein supply in ruminants using urinary purine derivative (ed.Makkar, H. P. S. and Chen, X. B.), pp.42–51FAO/IAEA, Kluwer Academic Publishers, Dordrecht.CrossRefGoogle Scholar
Makkar, H. P. S. 2004. Development, standardization and validation of nuclear based technologies for estimating microbial protein supply in ruminant livestock for improving productivity. In Estimation of microbial protein supply in ruminants using urinary purine derivative (ed.MakkarH. P. S., H. P. S., and Chen, X. B.), pp.2–13. Kluwer Academic Publishers, Dordrecht.CrossRefGoogle Scholar
Mead, R., Curnow, R. N. and Hasted, A. M. 1996. Statistical methods in agriculture and experimental biology, second edition. Chapman and Hall, London.Google Scholar
Moscardini, S., Haddi, M. L., Stefanon, B. and Susmel, P. 1999. Measurement of purine derivatives in the urine of some ruminant species. In Nuclear based technologies for estimating microbial protein supply in ruminant livestock IAEA-TECDOC-1093, pp. 111–118IAEA, Vienna.Google Scholar
Norton, B. W., Moran, J. B. and Nolan, J. V. 1979. Nitrogen metabolism in Brahman cross, buffaloes, Benteng and Shorthorn steers fed on low quality roughages. Australian Journal of Agricultural Research 30: 341–351.CrossRefGoogle Scholar
Ørskov, E. R. 1991. Protein nutrition in ruminants, second edition. Academic Press, London.Google Scholar
Ørskov, E. R., Benzie, D. and Kay, R. N. B. 1970. The effect of feeding procedure on closure of the esophageal groove in young sheep. British Journal of Nutrition 24: 785–795.CrossRefGoogle ScholarPubMed
Pimpa, O., Liang, J. B., Balcells, J., Jelan, Z. A. and Abdullah, N. 2002. Urinary purine derivative excretion in swarm buffaloes after duodenal purine base infusion. Animal Feed Science and Technology 104: 191–199.CrossRefGoogle Scholar
Soejono, M., Yusiati, L. M., Backrudin, Z., Budhi, S. P. S., Widyobroto, B. P. and Utomo, R. 2004. Flow of nucleic acids from the rumen and recovery of purine derivatives in the urine of cattle and buffaloes. In Estimation of microbial protein supply in ruminants using urinary purine derivative (ed.MakkarH. P. S., H. P. S., and ChenX. B., X. B.,), pp. 69–74FAO/IAEA, Kluwer Academic Publishers, Dordrecht.CrossRefGoogle Scholar
Vo, T. K. T., Dao, T. P., Tran, T. T. H., Phung, T. L., Ngo, M. D., Hoang, Q. H. and Ørskov, E. R. 2004. Comparison of purine derivative and creatinine in plasma and urine between cattle and buffaloes in Vietnam. In Estimation of microbial protein supply in ruminants using urinary purine derivative (ed. MakkarH. P. S., H. P. S., and ChenX. B., X. B.,), pp.75–85. FAO/IAEA, Kluwer Academic Publishers, Dordrecht.Google Scholar