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The activity of some digestive enzymes in domestic rabbits before and after weaning

Published online by Cambridge University Press:  02 September 2010

N. Dojană ,
M. Costache  and
A. Dinischiotu
N. Dojană
Affiliation:
Department of Biology of Laboratory Animals, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine, Bucharest, Romania
M. Costache
Affiliation:
Department of Enzymology, Faculty of Biology, University of Bucharest, Romania
A. Dinischiotu
Affiliation:
Department of Enzymology, Faculty of Biology, University of Bucharest, Romania
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Abstract

The activity ofamylase, maitase, lipase, pepsin, trypsin and chymotrypsin in suckling (15 days of age) and in 1-day weaned (43 days of age) domestic rabbits was assayed and compared with older (90 and 180 days) rabbits. It was found that amylase was active in the pancreas at 15 days (11 580 amylase units (AU) per mg protein) and increased during growth, reaching a maximum level (58 960 AU per mg protein) at 90 days of age. Specific activity (SA) of maitase from the small intestine mucosa varied depending on the intestinal segment and the age of the rabbits: activity in the duodenal mucosa decreased, while in the jejunal and Heal mucosa activity increased, during growth. Lipase SA reached a maximum level in suckling rabbits at the age of 15 days (in gastric mucosa 242 and in pancreas 608 mequiv. liberated oleic acid per mg protein per h, 37°C) and decreased sharply at weaning: in gastric mucosa down to 86 and in pancreas down to 89 mequiv. oleic acid per mg protein per h, 37°C. SA of pepsin remained relatively constant for all the studied categories of rabbits: 38 to 39 nmol tyrosine per mg protein per min, 25°C. Trypsin and chymotrypsin SA reached a peak at about weaning: 1·83 nmol benzoyl-arginyl-ethyl-ester per mg protein per min, 25°C and 40·1 nmol benzoyl-phenyl-naphtyl-ester per mg protein per min, 35·5°C respectively.

Keywords

digestive systemenzymesrabbitsweaning
Type
Research Article
Information
Animal Science , Volume 66 , Issue 2 , April 1998 , pp. 501 - 507
Copyright
Copyright © British Society of Animal Science 1998

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