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Susceptibility to pleuromutilins in Brachyspira (Serpulina) hyodysenteriae

Published online by Cambridge University Press:  28 February 2007

M. Karlsson ,
A. Gunnarsson  and
A. Franklin
M. Karlsson*
Affiliation:
Department of Antibiotics, National Veterinary Institute, Uppsala, Sweden
A. Gunnarsson
Affiliation:
Department of Bacteriology, National Veterinary Institute, Uppsala, Sweden
A. Franklin
Affiliation:
Department of Antibiotics, National Veterinary Institute, Uppsala, Sweden
*
*Department of Antibiotics, National Veterinary Institute, SE-751 89 Uppsala, Sweden. E-mail: [email protected]
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Abstract

The pleuromutilins are the only antimicrobial agents with sufficient minimum inhibitory concentration (MIC) values left to treat swine dysentery in Sweden. Other antimicrobials are either not approved for use against swine dysentery or only partly active against Brachyspira hyodysenteriae. To date, in Sweden two pleuromutilins, tiamulin and valnemulin, are authorized for use in pigs. This study includes a comparison between MICs of tiamulin and valnemulin for Swedish field isolates of B. hyodysenteriae, as determined by broth dilution. For different isolates the MIC of tiamulin was between 0 and 8 times higher than that of valnemulin. No resistance to pleuromutilins was recorded (tiamulin MIC range 0.031–2 μg/ml, valnemulin MIC range ≤0.016–1 μg/ml). In vitro development of tiamulin resistance was also studied. Two B. hyodysenteriae and two B. pilosicoli strains became resistant to tiamulin following reiterated passages on agar containing tiamulin in increasing concentrations. The resistance emerged slowly and three of the strains that went through more than 60 passages increased their tiamulin MICs from 0.031–0.25 to more than 128 μg/ml. The tiamulin MIC for one B. hyodysenteriaestrain that went through 29 passages increased from 0.0125 to 4 μg/ml. One B. pilosicoli strain developed cross-resistance to valnemulin; the MIC increased from 0.25 to more than 64 μg/ml. The valnemulin MIC for one B. hyodysenteriae strain increased from 0.031 μg/ml to 32 μg/ml. Valnemulin MIC was not determined for the B. hyodysenteriae strain that only went through 29 passages. The valnemulin MIC of the other B. pilosicoli strain increased from 0.031 to 4 μg/ml.

Type
Research Article
Information
Animal Health Research Reviews , Volume 2 , Issue 1 , June 2001 , pp. 59 - 66
Copyright
Copyright © CAB International 2001

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References

Adachi, Y, Hara, M, Hirano, K, Poomvises, P and Ingkaninun, P (1994). Biological properties of Serpulina hyodysenteriae and Serpulina innocens like organism with plasmid DNAs. In: Proceedings, 13th International Pig Veterinary Society Congress Bangkok, Thailand, p.147.Google Scholar
Aitken, IA, Morgan, JH, Dalziel, R, Burch, DG and Ripley, PH (1999). Comparative in vitro activity of valnemulin against porcine bacterial pathogens. Veterinary Record 144: 128.CrossRefGoogle ScholarPubMed
Böck, A, Turnowsky, F and Högenauer, G (1982). Tiamulin resistance mutations in Escherichia coli. Journal of Bacteriology 151: 1253–1260.CrossRefGoogle ScholarPubMed
Buller, NB and Hampson, DJ (1994). Antimicrobial susceptibility testing of Serpulina hyodysenteriae. Australian Veterinary Journal 71: 211–214.CrossRefGoogle ScholarPubMed
Cattani, P, Dettori, G, Calderaro, A, Grillo, R, Fadda, G and Chezzi, C (1998). Detection of extrachromosomal DNA in Italian isolates of weakly beta-haemolytic human intestinal spirochaetes. New Microbiologica 21: 241–248.Google ScholarPubMed
Combs, BG, Hampson, DJ and Harders, SJ (1992). Typing of Australian isolates of Treponema hyodysenteriae by serology and by DNA restriction endonuclease analysis. Veterinary Microbiology 31:273–285.CrossRefGoogle ScholarPubMed
Dorward, DW and Garon, CF (1990). DNA is packaged within membrane-derived vesicles of gram-negative but not gram-positive bacteria. Applied and Environmental Microbiology 56: 1960–1962.CrossRefGoogle Scholar
Drews, J, Georgopoulos, A, Laber, G, Schutze, E and Unger, J (1975). Antimicrobial activities of 81.723 hfu, a new pleu-romutilin derivative. Antimicrobial Agents and Chemotherapy 7: 507–516.CrossRefGoogle ScholarPubMed
Fellström, C and Gunnarsson, A (1995). Phenotypical characteri-sation of intestinal spirochaetes isolated from pigs. Research in Veterinary Science 59: 1–4.CrossRefGoogle ScholarPubMed
Gresham, AC, Hunt, BW and Dalziel, RW (1998). Treatment of swine dysentery –problems of antibiotic resistance and concurrent salmonellosis. Veterinary Record 143: 619.Google ScholarPubMed
Hayashi, T, Suenaga, I, Narukawa, N and Yamazaki, T (1988). In vitro and in vivo activities of sedecamycin against Treponema hyodysenteriae. Antimicrobial Agents and Chemotherapy 32: 458–461.CrossRefGoogle ScholarPubMed
Hodgin, LA and Högenauer, G (1974). The mode of action of pleuromutilin derivatives. Effect on cell-free polypeptide synthesis. European Journal of Biochemistry 47: 527–533.CrossRefGoogle ScholarPubMed
Högenauer, G (1975). The mode of action of pleuromutilin derivatives. Location and properties of the pleuromutilin binding site on Escherichia coli ribosomes. European Journal of Biochemistry 52: 93–98.CrossRefGoogle ScholarPubMed
Högenauer, G and Ruf, C (1981). Ribosomal binding region for the antibiotic tiamulin: stoichiometry, subunit location, and affinity for various analogs. Antimicrobial Agents and Chemotherapy 19: 260–265.CrossRefGoogle ScholarPubMed
Hommez, J, Castryck, F, Miry, C, Lein, A, Devriese, LA and Haesebrouck, F (1998). Susceptibility of different Serpulina species in pigs to antimicrobial agents. Vlaams Diergeneeskunding Tijdschrift 67: 32–35.Google Scholar
Humphrey, SB, Stanton, TB and Jensen, NS (1995). Mitomycin C induction of bacteriophages from Serpulina hyodysente-riae and Serpulina innocens. FEMS Microbiology Letters 134: 97–101.CrossRefGoogle ScholarPubMed
Humphrey, SB, Stanton, TB, Jensen, NS and Zuerner, RL (1997). Purification and characterization of VSH-1, a generalized transducing bacteriophage of Serpulina hyodysenteriae. Journal of Bacteriology 179: 323–329.CrossRefGoogle ScholarPubMed
Joens, LA, Margolin, AB and Hewlett, MJ (1986). The characterization of a plasmid isolated from Treponema hyodysenteriae and Treponema innocens. In: Proceedings, Annual Meeting of the American Society for Microbiology Washington DC, p.156.Google Scholar
Karlsson, M, Fellstrøm, C, Heldtander, MU, Johansson, KE and Franklin, A (1999). Genetic basis of macrolide and lin-cosamide resistance in Brachyspira (Serpulina) hyodysenteriae. FEMS Microbiology Letters 172: 255–260.CrossRefGoogle ScholarPubMed
Kavanagh, F, Hervey, A and Robbins, WJ (1951). Antibiotic substances from basidiomycetes. VIII. Pleurotus mutilis and Pleurotus passeckerianus. Proceedings of the National Academy of Sciences of the United States of America 37: 570–574.CrossRefGoogle Scholar
Kitai, K, Kashiwazaki, M, Adachi, Y, Kunugita, K and Arakawa, A (1987). In vitro antimicrobial activity against reference strains and field isolates of Treponema hyodysenteriae. Antimicrobial Agents and Chemotherapy 31: 1935–1938.CrossRefGoogle ScholarPubMed
Kunkle, RA, Harris, DL and Kinyon, JM (1986). Autoclaved liquid medium for propagation of Treponema hyodysenteriae. Journal of Clinical Microbiology 24: 669–671.CrossRefGoogle ScholarPubMed
Lucier, TS, Heitzman, K, Liu, SK and Hu, PC (1995). Transition mutations in the 23S rRNA of erythromycin-resistant isolates of Mycoplasma pneumoniae. Antimicrobial Agents and Chemotherapy 39: 2770–2773.CrossRefGoogle ScholarPubMed
Meier, A, Kirschner, P, Springer, B, Steingrube, VA, Brown, BA, Wallace, RJ Jr and Böttger, EC (1994). Identification of mutations in 23S rRNA gene of clarithromycin-resistant Mycobacterium intracellulare. Antimicrobial Agents and Chemotherapy 38: 381–384.CrossRefGoogle ScholarPubMed
Messier, S, Higgins, R and Moore, C (1990). Minimal inhibitory concentrations of five antimicrobials against Treponema hyodysenteriae and Treponema innocens. Journal of Veterinary Diagnostic Investigation 2: 330–333.CrossRefGoogle ScholarPubMed
Møller, K, Friis, NF, Meyling, A and Ripley, P (1996). In-vitro antimicrobial estimation of a new pleuromulin derivative, SDZ PMD 296, against Mycoplasma hyopneumoniae, Serpulina hyodysenteriae, and weakly beta-haemolytic spirochaetes. In: Proceedings, 14th International Pig Veterinary Society Congress Bologna, Italy, p.337.Google Scholar
Molnar, L (1996). Sensitivity of strains of Serpulina hyodysente-riae isolated in Hungary to chemotherapeutic drugs. Veterinary Record 138: 158–160.CrossRefGoogle ScholarPubMed
NCCLS (1997). Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria, 4th edn. Approved standard M11-A4. Wayne, Pennsylvania: National Committee for Clinical Laboratory Standards.Google Scholar
Ohmae, K, Yonezawa, S and Terakado, N (1983). Epizootiological studies on R plasmid with carbadox resistance. Nippon Juigaku Zasshi 45: 165–170.CrossRefGoogle Scholar
Ritchie, AE and Brown, LN (1971). An agent possibly associated with swine dysentery. Veterinary Record 89: 608–609.CrossRefGoogle ScholarPubMed
Ritchie, AE, Robinson, IM, Joens, LA and Kinyon, JM (1978). A bacteriophage for Treponema hyodysenteriae. Veterinary Record 103: 34–35.CrossRefGoogle ScholarPubMed
Rønne, H and Szancer, J (1990). In vitro susceptibility of Danish field isolates of Treponema hyodysenteriae to chemothera-peutics in swine dysentery (SD) therapy. In: Proceedings, 11th International Pig Veterinary Society Congress Lausanne, Switzerland, p.126.Google Scholar
Ross, JI, Eady, EA, Cove, JH, Jones, CE, Ratyal, AH, Miller, YW, Vyakrnam, S and Cunliffe, WJ (1997). Clinical resistance to erythromycin and clindamycin in cutaneous propionibac-teria isolated from acne patients is associated with mutations in 23S rRNA. Antimicrobial Agents and Chemotherapy 41: 1162–1165.CrossRefGoogle Scholar
Shibl, AM and Al-Sowaygh, IA (1979). Differential inhibition of bacterial growth and hemolysin production by lin-cosamide antibiotics. Journal of Bacteriology 137: 1022–1023.CrossRefGoogle ScholarPubMed
Shibl, AM and Gemmell, CG (1983). Effect of four antibiotics on haemolysin production and adherence to human uroepi-thelial cells by Escherichia coli. Journal of Medical Microbiology 16: 341–349.CrossRefGoogle ScholarPubMed
Shibl, AM, Ramadan, MA and Tawfik, AF (1994). Differential inhibition by clindamycin on slime formation, adherence to Teflon catheters and hemolysin production by Staphylococcus epidermidis. Journal of Chemotherapy 6: 107–110.CrossRefGoogle ScholarPubMed
Smith, SC, Muir, T, Holmes, M and Coloe, PJ (1991). In vitro antimicrobial susceptibility of Australian isolates of Treponema hyodysenteriae. Australian Veterinary Journal 68: 408–409.CrossRefGoogle ScholarPubMed
Stamm, LV and Bergen, HL (2000). A point mutation associated with bacterial macrolide resistance is present in both 23S rRNA genes of an erythromycin-resistant Treponema pal-lidum clinical isolate [letter]. Antimicrobial Agents and Chemotherapy 44: 806–807.CrossRefGoogle ScholarPubMed
Trott, DJ, Stanton, TB, Jensen, NS, Duhamel, GE, Johnson, JL and Hampson, DJ (1996). Serpulina pilosicoli sp. nov., the agent of porcine intestinal spirochetosis. International Journal of Systematic Bacteriology 46: 206–215.CrossRefGoogle ScholarPubMed
Turner, AK and Sellwood, R (1997). Extracellular DNA from Serpulina hyodysenteriae consists of 6.5 kbp random fragments of chromosomal DNA. FEMS Microbiology Letters 150: 75–80.CrossRefGoogle ScholarPubMed
Versalovic, J, Shortridge, D, Kibler, K, Griffy, MV, Beyer, J, Flamm, RK, Tanaka, SK, Graham, DY and Go, MF (1996). Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori. Antimicrobial Agents and Chemotherapy 40: 477–480.CrossRefGoogle ScholarPubMed
Weber, FH and Earley, DL (1991). Novel method for measuring growth of Treponema hyodysenteriae and its application for monitoring susceptibility of clinical isolates to antimicrobial agents. Antimicrobial Agents and Chemotherapy 35: 2012–2015.CrossRefGoogle ScholarPubMed
Wexler, HM (1991). Susceptibility testing of anaerobic bacteria: myth, magic, or method? Clinical Microbiology Reviews 4: 470–484.CrossRefGoogle ScholarPubMed
Zuerner, RL and Stanton, TB (1994). Physical and genetic map of the Serpulina hyodysenteriae B78Tchromosome. Journal of Bacteriology 176: 1087–1092.CrossRefGoogle Scholar