Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-24T07:59:15.326Z Has data issue: false hasContentIssue false
  • English
  • Français

Diagnosis of Mycoplasma hyopneumoniae

Published online by Cambridge University Press:  28 February 2007

Eileen L. Thacker
Eileen L. Thacker*
Affiliation:
2118 Veterinary Medicine Building, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
*
E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Mycoplasma hyopneumoniae, the cause of enzootic pneumonia, remains an important pathogen in the swine industry. This small, complex organism colonizes the ciliated cells of the respiratory tract, resulting in little exposure to the immune system. Confirming the presence of M. hyopneumoniae, as well as identifying its role in respiratory disease and pneumonia, remains challenging to the veterinary profession. While culture of the organism remains the gold standard for identification, the use of serology, the polymerase chain reaction and various assays to detect the presence of M. hyopneumoniae in tissue is common in diagnostic laboratories. Because of the role M. hyopneumoniae plays in increasing the severity of pneumonia associated with concurrent bacterial and viral infections, understanding the pathogenesis and diagnostic assays available is critical for developing effective intervention strategies to control respiratory disease on a herd basis.

Keywords

Mycoplasma hyopneumoniaediagnosispigs
Type
Research Article
Information
Animal Health Research Reviews , Volume 5 , Issue 2 , December 2004 , pp. 317 - 320
Copyright
Copyright © CAB International 2004

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Amanfu, W, Weng, CN, Ross, RF and Barnes, HJ (1984). Diagnosis of mycoplasmal pneumoniae of swine: sequential study by direct immunofluorescence. American Journal of Veterinary Research 45: 1349–1352.Google ScholarPubMed
Amass, SF, Clark, LK, van Alstine, WG, Bowersock, TL, Murphy, DA, Knox, KE and Albregts, SR (1994). Interaction of Mycoplasma hyopneumoniae and Pasteurella multocida infections in swine. Journal of the American Veterinary Medical Association 204: 102–107.CrossRefGoogle ScholarPubMed
Artiushin, S, Stipkovits, L and Minion, FC (1993). Development of polymerase chain reaction primers to detect Mycoplasma hyopneumoniae. Molecular and Cellular Probes 7: 381–385.CrossRefGoogle ScholarPubMed
Bereiter, M, Young, TF, Joo, HS and Ross, RF (1990). Evaluation of the ELISA and comparison to the complement fixation test and radial immunodiffusion enzyme assay for detection of antibodies against Mycoplasma hyopneumoniae in swine serum. Veterinary Microbiology 25: 177–192.CrossRefGoogle Scholar
Calsamiglia, M and Pijoan, C (1998). Shedding of Mycoplasma hyopneumoniae in sows of different parities. In: Proceedings of the 15th IPVS Congress p. 146Google Scholar
Calsamiglia, M and Pijoan, C (2000). Colonisation state and colostral immunity to Mycoplasma hyopneumoniae of different parity sows. Veterinary Record 146: 530–532.CrossRefGoogle ScholarPubMed
Ciprian, A, Cruz, TA and de la Garza, M (1994). Mycoplasma hyopneumoniae: interaction with other agents in pigs, and evaluation of immunogens. Archives of Medical Research 25: 235–239.Google Scholar
DeBey, MC and Ross, RF (1994). Ciliostasis and loss of cilia induced by Mycoplasma hyopneumoniae in porcine tracheal organ cultures. Infection and Immunity 62: 5312–5318.CrossRefGoogle ScholarPubMed
DeBey, MC, Jacobson, CD and Ross, RF (1992). Histochemical and morphologic changes of porcine airway epithelial cells in response to infection with Mycoplasma hyopneumoniae. American Journal of Veterinary Research 53: 1705–1710.CrossRefGoogle ScholarPubMed
Djordjevic, SP, Eamens, GJ, Romalis, LF, Nicholls, PJ, Taylor, V and Chin, J (1997). Serum and mucosal antibody responses and protection in pigs vaccinated against Mycoplasma hyopneumoniae with vaccines containing a denatured membrane antigen pool and adjuvant. Australian Veterinary Journal 75: 504–511.CrossRefGoogle ScholarPubMed
Erlandson, K, Thacker, B, Wegner, M, Evans, R and Thacker, E (2002). Evaluation of three serum antibody ELISA tests for Mycoplasma hyopneumoniae. In: 17th Congress of the International Pig Veterinary Society 2 p. 74Google Scholar
Friis, NF (1975). Some recommendations concerning primary isolations of Mycoplasma suipneumoniae and Mycoplasma flocculare. Nordic Veterinary Medicine 27: 337–339.Google ScholarPubMed
Kurth, KT, Hsu, T, Snook, ER, Thacker, EL, Thacker, BJ and Minion, FC (2002). Use of a Mycoplasma hyopneumoniae nested polymerase chain reaction test to determine the optimal sampling sites in swine. Journal of Veterinary Diagnostic Investigation 14: 463–469.CrossRefGoogle ScholarPubMed
Kwon, D and Chae, C (1999). Detection and localization of Mycoplasma hyopneumoniae DNA in lungs from naturally infected pigs by in situ hybridization using a digoxigenin-labeled probe. Veterinary Pathology 36: 308–313.CrossRefGoogle ScholarPubMed
Maes, D, Deluyker, H, Verdonck, M, Castryck, F, Miry, C, Vrijens, B and de Kruif, A (1999). Risk indicators for the seroprevalence of Mycoplasma hyopneumoniae, porcine influenza viruses and Aujeszky's disease virus in slaughter pigs from fattening pig herds. Zentralblatt für Veterinarmedizin B 46: 341–352.Google ScholarPubMed
Opriessnig, T, Thacker, E, Yu, S, Fenaux, M, Meng, J, Halbur, P (in press) Experimental reproduction of postweaning multisystemic wasting syndrome by dual-infection of conventional pigs with Mycoplasma hyopneumoniae and porcine circovirus type 2. Veterinary Pathology in press.Google Scholar
Ross, R (1992). Mycoplasmal diseases. In: Leman, AD, Straw, B and Mengeling, WL editors. Diseases of Swine Ames (IA): Iowa State University Press pp. 537–551Google Scholar
Sorensen, V, Barfod, K, Ahrens, P, Friis, NF, Feenstra, AA, Pedersen, MW, Feld, NC and Jensen, NE (1994). Comparison of four different methods for demonstration of Mycoplasma hyopneumoniae in lungs of experimentally inoculated pigs. Proceedings of the 13th IPVS Congress p. 188. The Hague NetherlandsGoogle Scholar
Sorensen, V, Ahrens, P, Barfod, K, Feenstra, AA, Feld, NC, Friis, NF, Bille-Hansen, V, Jensen, NE and Pedersen, MW (1997). Mycoplasma hyopneumoniae infection in pigs: duration of the disease and evaluation of four diagnostic assays. Veterinary Microbiology 54: 23–34.CrossRefGoogle ScholarPubMed
Strait, E, Madsen, M, Minion, F and Thacker, E (2003). Analysis of Mycoplasma hyopneumoniae field isolates. In: 84th Annual Meeting of the Conference of Researchers of Animal Diseases p. 13Google Scholar
Thacker, EL, Thacker, BJ, Boettcher, TB and Jayappa, H (1998). Comparison of antibody production, lymphocyte stimulation, and protection induced by four commercial Mycoplasma hyopneumoniae bacterins. Swine Health and Production 6: 107–112.Google Scholar
Thacker, EL, Halbur, PG, Ross, RF, Thanawongnuwech, R and Thacker, BJ (1999a). Mycoplasma hyopneumoniae potentiation of porcine reproductive and respiratory syndrome virus-induced pneumonia. Journal of Clinical Microbiology 37: 620–627.CrossRefGoogle ScholarPubMed
Thacker, EL, Halbur, PG and Thacker, BJ (1999b). Effect of vaccination on dual infection with Mycoplasma hyopneumoniae and PRRSV. In: Proceedings of the of the American Association of Swine Practitioners p. 375–377Google Scholar
Thacker, EL, Thacker, BJ, Young, TF and Halbur, PG (2000). Effect of vaccination on the potention of porcine reproductive and respiratory syndrome virus (PRRSV)-induced pneumonia by Mycoplasma hyopneumoniae. Vaccine 18: 1244–1252.CrossRefGoogle Scholar
Thacker, EL, Thacker, BJ and Janke, BH (2001). Interaction between Mycoplasma hyopneumoniae and swine influenza virus. Journal of Clinical Microbiology 39: 2525–2530.CrossRefGoogle ScholarPubMed
Verdin, E, Saillard, C, Labbe, A, Bove, JM and Kobisch, M (2000). A nested PCR assay for the detection of Mycoplasma hyopneumoniae in tracheobronchiolar washings from pigs. Veterinary Microbiology 76: 31–40.CrossRefGoogle ScholarPubMed
Young, TF and Ross, RF (1987). Assessment of antibody response of swine infected with Mycoplasma hyopneumoniae by immunoblotting. American Journal of Veterinary Research 48: 651–656.Google ScholarPubMed