Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-25T18:24:43.481Z Has data issue: false hasContentIssue false
  • English
  • Français

Development and application of molecular diagnostics and proteomics to bovine respiratory disease (BRD)

Published online by Cambridge University Press:  02 December 2020

John Dustin Loy Open the ORCID record for John Dustin Loy [Opens in a new window]
John Dustin Loy*
Affiliation:
4040 East Campus Loop, 115Q NVDC, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE68583-0906, USA
*
Author for correspondence: John Dustin Loy, 4040 East Campus Loop, 115Q NVDC, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE68583-0906, USA. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Advances in molecular and proteomic technologies and methods have enabled new diagnostic tools for bovine respiratory pathogens that are high-throughput, rapid, and extremely sensitive. Classically, diagnostic testing for these pathogens required culture-based approaches that required days to weeks and highly trained technical staff to conduct. However, new advances such as multiplex hydrolysis probe-based real-time PCR technology have enabled enhanced and rapid detection of bovine respiratory disease (BRD) pathogens in a variety of clinical specimens. These tools provide many advantages and have shown superiority over culture for co-infections/co-detections where multiple pathogens are present. Additionally, the integration of matrix-assisted laser desorption ionization time of flight mass spectrometry (MS) into veterinary diagnostic labs has revolutionized the ability to rapidly identify bacterial pathogens associated with BRD. Recent applications of this technology include the ability to type these opportunistic pathogens to the sub-species level (specifically Mannheimia haemolytica) using MS-based biomarkers, to allow for the identification of bacterial genotypes associated with BRD versus genotypes that are more likely to be commensal in nature.

Keywords

BRD diagnosisMALDI typingMALDI-TOF MSMannheimia haemolyticareal-time PCR bovine respiratory disease
Type
Special issue: Papers from Bovine Respiratory Disease Symposium
Information
Animal Health Research Reviews , Volume 21 , Issue 2 , December 2020 , pp. 164 - 167
Check for updates
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bell, CJ, Blackburn, P, Elliott, M, Patterson, TI, Ellison, S, Lahuerta-Marin, A and Ball, HJ (2014) Investigation of polymerase chain reaction assays to improve detection of bacterial involvement in bovine respiratory disease. Journal of Veterinary Diagnostic Investigation 26, 631–634.CrossRefGoogle ScholarPubMed
Berensmeier, S (2006) Magnetic particles for the separation and purification of nucleic acids. Applied Microbiology and Biotechnology 73, 495–504.CrossRefGoogle ScholarPubMed
Booker, CW, Abutarbush, SM, Morley, PS, Jim, GK, Pittman, TJ, Schunicht, OC, Perrett, T, Wildman, BK, Fenton, RK, Guichon, PT and Janzen, ED (2008) Microbiological and histopathological findings in cases of fatal bovine respiratory disease of feedlot cattle in western Canada. The Canadian Veterinary Journal 49, 473–481.Google ScholarPubMed
Capik, SF, White, BJ, Lubbers, BV, Apley, MD, Mosier, DA, Larson, RL and Murray, RW (2015) Characterization of Mannheimia haemolytica in beef calves via nasopharyngeal culture and pulsed-field gel electrophoresis. Journal of Veterinary Diagnostic Investigation 27, 568–575.CrossRefGoogle ScholarPubMed
Clark, AE, Kaleta, EJ, Arora, A and Wolk, DM (2013) Matrix-assisted laser desorption ionization–time of flight mass spectrometry: a fundamental shift in the routine practice of clinical microbiology. Clinical Microbiology Reviews 26, 547–603.CrossRefGoogle ScholarPubMed
Clawson, ML, Murray, RW, Sweeney, MT, Apley, MD, Dedonder, KD, Capik, SF, Larson, RL, Lubbers, BV, White, BJ, Kalbfleisch, TS, Schuller, G, Dickey, AM, Harhay, GP, Heaton, MP, Chitko-Mckown, CG, Brichta-Harhay, DM, Bono, JL and Smith, TPL (2016) Genomic signatures of Mannheimia haemolytica that associate with the lungs of cattle with respiratory disease, an integrative conjugative element, and antibiotic resistance genes. BMC Genomics 17, 982.CrossRefGoogle ScholarPubMed
Fulton, RW and Confer, AW (2012) Laboratory test descriptions for bovine respiratory disease diagnosis and their strengths and weaknesses: gold standards for diagnosis, do they exist? The Canadian Veterinary Journal = La revue veterinaire canadienne 53, 754–761.Google ScholarPubMed
Fulton, RW, Blood, KS, Panciera, RJ, Payton, ME, Ridpath, JF, Confer, AW, Saliki, JT, Burge, LT, Welsh, RD, Johnson, BJ and Reck, A (2009) Lung pathology and infectious agents in fatal feedlot pneumonias and relationship with mortality, disease onset, and treatments. Journal of Veterinary Diagnostic Investigation 21, 464–477.CrossRefGoogle ScholarPubMed
Fulton, RW, D'offay, JM, Landis, C, Miles, DG, Smith, RA, Saliki, JT, Ridpath, JF, Confer, AW, Neill, JD, Eberle, R, Clement, TJ, Chase, CCL, Burge, LJ and Payton, ME (2016) Detection and characterization of viruses as field and vaccine strains in feedlot cattle with bovine respiratory disease. Vaccine 34, 3478–3492.CrossRefGoogle ScholarPubMed
Griffin, D, Chengappa, MM, Kuszak, J and Mcvey, DS (2010) Bacterial pathogens of the bovine respiratory disease complex. Veterinary Clinics of North America: Food Animal Practice 26, 381–394.Google ScholarPubMed
Harhay, GP, Harhay, DM, Bono, JL, Smith, TPL, Capik, SF, Dedonder, KD, Apley, MD, Lubbers, BV, White, BJ and Larson, RL (2017) Closed genome sequences of seven Histophilus somni isolates from beef calves with bovine respiratory disease complex. Genome Announcements 5, e01099–17.CrossRefGoogle ScholarPubMed
Horwood, PF and Mahony, TJ (2011) Multiplex real-time RT-PCR detection of three viruses associated with the bovine respiratory disease complex. Journal of Virological Methods 171, 360–363.CrossRefGoogle ScholarPubMed
Johnston, D, Earley, B, Cormican, P, Murray, G, Kenny, DA, Waters, SM, Mcgee, M, Kelly, AK and Mccabe, MS (2017) Illumina MiSeq 16S amplicon sequence analysis of bovine respiratory disease associated bacteria in lung and mediastinal lymph node tissue. BMC Veterinary Research 13, 118.CrossRefGoogle ScholarPubMed
Lauerman, L (1998) Mycoplasma PCR assays: detection by PCR amplification. In Lauerman LH (ed), Nucleic Acid Amplification Assays for Diagnosis of Animal Diseases. Visalia, CA, USA: American Association of Veterinary Laboratory Diagnosticians, pp. 1–134.Google Scholar
Loy, JD and Clawson, ML (2017) Rapid typing of Mannheimia haemolytica major genotypes 1 and 2 using MALDI-TOF mass spectrometry. Journal of Microbiological Methods 136, 30–33.CrossRefGoogle ScholarPubMed
Loy, JD, Leger, L, Workman, AM, Clawson, ML, Bulut, E and Wang, B (2018 a) Development of a multiplex real-time PCR assay using two thermocycling platforms for detection of major bacterial pathogens associated with bovine respiratory disease complex from clinical samples. Journal of Veterinary Diagnostic Investigation 30, 837–847.CrossRefGoogle ScholarPubMed
Loy, JD, Payne, J, Deal, C, Dutta, E, Bulut, E, Clawson, ML and Wang, B (2018 b) Moving beyond the MIC: evaluation of a novel multiplex real time PCR assay for detection of antimicrobial resistance genes in clinical bovine respiratory disease samples. 61st Annual Conference of the American Association of Veterinary Laboratory Diagnosticians, October 20, 2018 Kansas, City, MO. 7.Google Scholar
Lubbers, BV and Hanzlicek, GA (2013) Antimicrobial multidrug resistance and coresistance patterns of Mannheimia haemolytica isolated from bovine respiratory disease cases – a three-year (2009–2011) retrospective analysis. Journal of Veterinary Diagnostic Investigation 25, 413–417.CrossRefGoogle Scholar
Mani, RJ, Thachil, AJ and Ramachandran, A (2017) Discrimination of Streptococcus equi subsp. equi and Streptococcus equi subsp. zooepidemicus using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Journal of Veterinary Diagnostic Investigation 29, 622–627.CrossRefGoogle ScholarPubMed
Masri, SA, Olson, W, Nguyen, PT, Prins, S and Deregt, D (1996) Rapid detection of bovine herpesvirus 1 in the semen of infected bulls by a nested polymerase chain reaction assay. Canadian Journal of Veterinary Research = Revue canadienne de recherche veterinaire 60, 100–107.Google ScholarPubMed
Pérez-Sancho, M, Vela, AI, Horcajo, P, Ugarte-Ruiz, M, Domínguez, L, Fernández-Garayzábal, JF and De La Fuente, R (2018) Rapid differentiation of Staphylococcus aureus subspecies based on MALDI-TOF MS profiles. Journal of Veterinary Diagnostic Investigation 30, 813–820.CrossRefGoogle ScholarPubMed
Reynisson, E, Josefsen, MH, Krause, M and Hoorfar, J (2006) Evaluation of probe chemistries and platforms to improve the detection limit of real-time PCR. Journal of Microbiological Methods 66, 206–216.CrossRefGoogle ScholarPubMed
Schmitt, BJ, Lopez, OJ, Ridpath, JF, Galeota-Wheeler, J and Osorio, FA (1994) Evaluation of PCR for diagnosis of bovine viral diarrhea virus in tissue homogenates. Journal of Veterinary Diagnostic Investigation 6, 44–47.CrossRefGoogle ScholarPubMed
Seng, P, Drancourt, M, Gouriet, F, La Scola, B, Fournier, P-E, Rolain, JM and Raoult, D (2009) Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clinical Infectious Diseases 49, 543–551.CrossRefGoogle ScholarPubMed
Tegtmeier, C, Angen, Ø and Ahrens, P (2000) Comparison of bacterial cultivation, PCR, in situ hybridization and immunohistochemistry as tools for diagnosis of Haemophilus somnus pneumonia in cattle. Veterinary Microbiology 76, 385–394.CrossRefGoogle ScholarPubMed
Timsit, E, Workentine, M, Van Der Meer, F and Alexander, T (2018) Distinct bacterial metacommunities inhabit the upper and lower respiratory tracts of healthy feedlot cattle and those diagnosed with bronchopneumonia. Veterinary Microbiology 221, 105–113.CrossRefGoogle ScholarPubMed
Vilcek, S, Elvander, M, Ballagi-Pordány, A and Belák, S (1994) Development of nested PCR assays for detection of bovine respiratory syncytial virus in clinical samples. Journal of Clinical Microbiology 32, 2225–2231.CrossRefGoogle ScholarPubMed
Woolums, AR, Karisch, BB, Frye, JG, Epperson, W, Smith, DR, Blanton, J Jr, Austin, F, Kaplan, R, Hiott, L, Woodley, T, Gupta, SK, Jackson, CR and Mcclelland, M (2018) Multidrug resistant Mannheimia haemolytica isolated from high-risk beef stocker cattle after antimicrobial metaphylaxis and treatment for bovine respiratory disease. Veterinary Microbiology, 221, 143–152.CrossRefGoogle ScholarPubMed
Workman, AM, Kuehn, LA, Mcdaneld, TG, Clawson, ML, Chitko-Mckown, CG and Loy, JD (2017) Evaluation of the effect of serum antibody abundance against bovine coronavirus on bovine coronavirus shedding and risk of respiratory tract disease in beef calves from birth through the first five weeks in a feedlot. American Journal of Veterinary Research 78, 1065–1076.CrossRefGoogle Scholar
Workman, AM, Kuehn, LA, Mcdaneld, TG, Clawson, ML and Loy, JD (2019) Longitudinal study of humoral immunity to bovine coronavirus, virus shedding, and treatment for bovine respiratory disease in pre-weaned beef calves. BMC Veterinary Research 15, 161.CrossRefGoogle ScholarPubMed