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The use of DNA markers in deciding conservation priorities in sheep and other livestock

Published online by Cambridge University Press:  01 August 2011

A. M. Crawford  and
R.P. Littlejohn
A. M. Crawford
Affiliation:
AgResearch Molecular Biology Unit, Dept of Biochemistry and Centre for Gene Research, University of Otago, PO Box 56, Dunedin, New Zealand
R.P. Littlejohn
Affiliation:
AgResearch Invermay Agricultural Centre Private Bag 50034, Mosgiel, New Zealand
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Summary

The genetic diversity of most livestock species is reducing and it is not possible to preserve all livestock breeds. In order to preserve as much of the genetic diversity as possible we must first have a robust method of measuring the genetic differences between breeds. The analysis of microsatellite allele frequency is now the method of choice. Using sheep as an example, this paper describes the methods used for both microsatellite amplification and the analysis of the data once it has been collected.

Resumen

La diversidad genética de muchas especies ganaderas ha quedado muy reducida y no es posible preservar todas las razas. Para poder preservar el mayor número posible de razas, debemos, en primer lugar, poseer un método riguroso de medida de las diferencias genéticas existentes entre razas. El análisis de la frecuencia alálica de microsatélites es hoy en día un método importante. Tomando como ejemplo los ovinos, este artéculo describe los métodos utilizados para la amplificacion de microsatélites y el análisis de datos una vez recogidos.

Keywords

Microsatellitesgenome mappinglinkageDNAGenetic distance
Type
Research Articles
Information
Animal Genetic Resources/Resources génétiques animales/Recursos genéticos animales , Volume 23 , April 1998 , pp. 21 - 26
Copyright
Copyright © Food and Agriculture Organization of the United Nations 1998

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