Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-25T00:55:11.407Z Has data issue: false hasContentIssue false
  • English
  • Français

Strukturelle Chromosomenaberrationen in den Lymphozyten gesunder Probanden unter dem Einfluss verschiedener Kulturbedingungen

Published online by Cambridge University Press:  01 August 2014

Hannes Meist
Hannes Meist*
Affiliation:
Institut für Humangenetik und Anthropologie der, Universität Erlangen-Nürnberg
*
Institut für Humangenetik und Anthropologie der, Universität Erlangen-Nürnberg, Deutschland

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The frequency of structural chromosomal aberrations has been examined in peripheral blood lymphocyte cultures from 18 normal individuals. A mean rate of aberrations was observed of 7.38%, and a mean breakage/metaphase index of 0.036. Individual values ranged, for the aberration index, from 4% to 15%; and, for the breakage/metaphase index, from 0.00 to 0.08. Only gaps and breakage were observed. The translocation cross and chromatid-ring observed derive from a series of experiments on homologous plasma cultures. As for the influence of different cultural environments, it has been observed that the rate of spontaneous aberrations behaved independently from the age of the blood sample, from the addition of penicillin in the culture, and from the concentration of phytohemoagglutinin. Using veal's fetal serum, the rate of aberrations failed to show any variation, even with a time of culture prolonged up to 96 hours; while, using homologous plasma, the time of culture having been prolonged, structural aberrations have shown a considerable increase. The study of the mitotic index in the different cultural conditions has indicated the possibility of favoring the growth of leucocyte cultures.

Type
Research Article
Information
Acta geneticae medicae et gemellologiae: twin research , Volume 20 , Issue 2 , April 1971 , pp. 174 - 188
Copyright
Copyright © The International Society for Twin Studies 1970

References

Literatur

Allison, Paton 1965. Chromosome damage in human diploid cells following activation of lysosomal enzymes. Nature (London), 207: 1170.CrossRefGoogle ScholarPubMed
Barthelmess, A. 1963. Möglichkeit und Gelegenheiten zur Schädigung des Erbgutes beim Menschen durch mutagene Stoffe; ein Vorschlag zur Erforschung. Proc. 2nd Int. Congr. Hum. Genet., 2: 1251.Google Scholar
Bender, M. A., Gooch, P. G. 1962. Types and rates of X-ray-induced chromosome aberrations in human blood irradiated in vitro. Genetics, 48: 522.Google ScholarPubMed
Bishun, N. P. 1967. Comparison of mitotic growth rates of human capillary whole blood cultured in a variety of media. J. Med. Genet., 4: 41–43.CrossRefGoogle Scholar
Bochkov, N. P., Kolzlov, V. M. Pilosov, R. A., Sevankaev, A. V. 1968. The level of spontaneous chromosome aberrations in cultures of human leukocytes. Genetika (Leningrad), 4: 93.Google Scholar
Cohen, M. M. 1963. The specific effect of streptonigrin activity on human chromosomes in culture. Cytogenetics (Basel), 2: 271.CrossRefGoogle ScholarPubMed
Cohen, M. M., Shaw, M. W. (1964). Effects of mytomycin C on human chromosomes. J. Cell. Biol., 23: 386.CrossRefGoogle Scholar
Court Brown, W. M., Buckton, K. E., Jacobs, P. A., Tough, E. M., Kuenssberg, E. V., Knox, J. D. E. 1966. Chromosome Studies on Adults. Cambridge University Press, London.Google Scholar
Gebhart, E. 1968. Untersuchungen über die Wirkung von 8-Hydroxychinoliniumsulfat auf menschliche Chromosomen in vitro. Mutat. Res., 6: 309–318.CrossRefGoogle ScholarPubMed
Gebhart, E. 1969. Chromosomenaberrationen durch Myleran in menschlichen Leukozyten in vitro. Humangenetik, 7: 126–136.Google Scholar
Hampel, K. E. 1968. Über die Wirkung von Cytostatika auf die Chromosomen des Menschen. Int. J. Clin. Pharm. Tox., 14: 322–371.Google Scholar
Honda, T., Kamada, N., Bloom, A. D. 1969. Chromosome aberrations and culture time. Cytogenetics (Basel), 8: 117.CrossRefGoogle ScholarPubMed
Jackson, J. F. 1964. Chromosome aberrations in cultured human leukocytes treated with 8-ethoxycaffeine. J. Cell. Biol., 22: 291–293.CrossRefGoogle ScholarPubMed
Kihlman, B. A., Nichols, W., Levan, A. 1963. The effect of desoxyadenosine and cytosinearabinoside on the chromosomes of human leukocytes in vitro. Hereditas (Lund), 50: 139.CrossRefGoogle Scholar
Kurita, Y., Yosida, T. H., Moriwaki, K. 1965. Nonrandomness in the distribution of chromosome aberrations induced by a radiomimetic chemical, 4-nitroquinoline-1-oxide, in tumor cells. Jap. J. Genet., 40: 365–376.CrossRefGoogle Scholar
Lubs, H.A., Samuelson, J. 1967. Chromosome abnormalities in lymphocytes from normal human subjects. Cytogenetics (Basel), 6: 402–411.CrossRefGoogle ScholarPubMed
Makino, S., Awa, A. A., Sasaki, M. 1968. Chromosome studies in normal human subjects. Ann. N. Y. Acad. Sci., 155: 679–694.CrossRefGoogle Scholar
Moore, J. G., Campenhout, J. L. van, Brandkamp, W. M. (1964). Effects of ionizing irradiation and chemotherapeutic agents on human chromosomes. Amer. J. Obstet. Gynec, 88: 985.CrossRefGoogle ScholarPubMed
Moorhead, P. S., Nowell, P. C., Mellman, W. J., Battips, D. M., Hungerford, D. A. (1960). Chromosome preparations of leukocytes cultured from human peripheral blood. Exp. Cell. Res., 20: 613.CrossRefGoogle ScholarPubMed
Neu, R., Aspillaga, J., Gardener, L. I. 1965. Effects of antibiotics on chromosomes of cultured human leukocytes. Nature (London), 205: 171.CrossRefGoogle Scholar
Nichols, W. W., Levan, A., Hall, B., Östergren, G. 1962. Measles-associated chromosome breakage. Hereditas (Lund), 48: 367.CrossRefGoogle Scholar
Nichols, W. W. (1964). In vitro chromosome breakage induced by arabinosyladenosine in human leukocytes. Cancer Res., 24: 1502.Google Scholar
Nichols, W. W., Levan, A., Heneen, W. K. 1967. Studies on the role of viruses in somatic mutation. Hereditas (Lund), 57: 366.Google ScholarPubMed
Rieger, R., Michaelis, A. 1967. Chromosomenmutationen. Fischer, Jena.Google Scholar
Vogel, F., Röhrborn, G., Schleiermacher, E., Schröder, T. M. 1967. Mutationen durch chemische EinWirkung bei Säuger und Mensch. Deutsch. Med. Wschr., 92: 2249.CrossRefGoogle Scholar