Late Cytogenetic Changes after Treatment of Malignancy

Extract

A 30-year-old man with azoospermia was routinely investigated in the Department of Clinical Genetics at our hospital. His karyotype was analysed after phytohaemagglutinin stimulation of lymphocytes following short-term cultivation of peripheral blood.

Taking his history, it was ascertained that in childhood, Hodgkin's disease had been confirmed histologically. He began treatment at 7 years, receiving several series of combined cytostatic treatment supplemented with X-ray therapy. The patient's history is summarized in table 1.

For the last 14 years, the patient has felt well. He is not taking medication and has no problems with primary disease. He is examined by an oncologist twice a year (including scintigraphy) with negative findings. He has no complications, except for azoospermia.

The karyotype was analysed using standard methods. The chromosomes were stained by Giemsa following G-banding by the trypsin method.