Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-24T08:20:15.907Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunohistochemical Analysis of c-Fos and c-Jun in Retinoblastoma

Published online by Cambridge University Press:  01 August 2014

F. Faraldi ,
A. Calzolari ,
M. Forni ,
F.L. Chiarelli Mincione  and
G. Alfieri
F. Faraldi*
Affiliation:
Istituto di Clinica Oculistica e
A. Calzolari
Affiliation:
Istituto di Anatomia e Istologia Patologica dell'Università degli Studi di Firenze, Firenze, Italy
M. Forni
Affiliation:
Divisione di Anatomia Patologica - Ospedale Regina Margherita, Torino, Italy
F.L. Chiarelli Mincione
Affiliation:
Istituto di Clinica Oculistica e
G. Alfieri
Affiliation:
Istituto di Clinica Oculistica e
*
Clinica Oculistica II, Viale Morgagni 85, I-50141 Firenze, Italy

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The c-fos promoter is negatively regulated by the retinoblastoma (Rb)-suceptibility-gene- encoded protein as well as by other genes involved in the control of transcription, cell cycle regulation and neoplastic transformation. We have examined by immunohistochemistry the c-Fos and c-Jun proteins in five cases of retinoblastoma in order to evaluate eventual alterations in their expression in vivo, possibly related to a gene mutation or to loss of Rb negative control.

Keywords

RetinoblastomaHumanImmunohistochemistryC-FosC-Jun
Type
Research Article
Information
Acta geneticae medicae et gemellologiae: twin research , Volume 45 , Issue 1-2 , April 1996 , pp. 289 - 291
Copyright
Copyright © The International Society for Twin Studies 1996

References

REFERENCES

1. Friend, SH, Horowitz, JM, Gerber, MR, Wang, X, Bogenmann, E, Li, FP, Weimberg, RA: Deletions of a DNA sequence in retinoblastoma and mesenchymal tumors: Organization of the sequence and its encoded protein. Proc Natl Acad Sci USA 1987; 84: 9059–9063.Google Scholar
2. Horowitz, JM, Park, SH, Bogenmann, E, Cheng, JC, Yandell, DW, Kaye, FJ, Minna, JD, Dryia, TP, Weinberg, RA: Frequent inactivation of the retinoblastoma anti-oncogene is restricted to a subset of human tumor cells. Proc Natl Acad Sci USA 1990; 87: 2775–2779.Google Scholar
3. Weimberg, R: Tumor suppressor genes. Science 1991; 254: 1138–1146.CrossRefGoogle Scholar
4. De Caprio, JA, Furukawa, J, Ajchenbaum, F, Griffin, JD, Livingstom, DM: The retinoblastoma susceptibility gene product becomes phosphorylated in multiple stages during cell cycle entry and progression. Proc Nat Acad Sci USA 1992; 89: 1795–1798.Google Scholar
5. Huang, HSJ, Yee, JK, Shew, JY, Chen, PL, Bookstein, R, Friedmann, T, Lee, EYHP and Lee, WH: Suppression of the neoplastic phenotype by replacement of the retinoblastoma gene product in human cancer cells. Science 1988; 242: 1563–1566.Google Scholar
6. Bookstein, R, Shew, JY, Chen, PL, Scully, P, Lee, WH: Suppression of tumorigenicity of human prostate carcinoma cells by replacing a mutated Rb gene. Science 1990; 247: 712–715.CrossRefGoogle ScholarPubMed
7. Lee, WH, Shew, JY, Hong, FD, Sery, T, Donoso, LA, Young, LJ, Bookstein, R, Lee, EYHP: Human retinoblastoma susceptibility gene product is a nuclear phosphoprotein associated with DNA binding activity. Nature 1987; 329: 642–645.CrossRefGoogle ScholarPubMed
8. Udvadia, AJ, Rogers, KT, Higgins, PDR, Murata, Y, Martin, KH, Humphrey, PA, Horowitz, JM: Sp-1 bind promoter elements regulated by the RB protein and Sp-1 mediated transcription is stimulated by RB coexpression. Proc Natl Acad Sci USA 1993; 90: 3265–3269.Google Scholar
9. Robbins, PD, Horowitz, JM, Mulligan, RC: Negative regulation of human c-fos expression by the retinoblastoma gene product. Nature 1990; 346: 668–671.Google Scholar
10. Bishop, M: Molecular themes in oncogenesis. Cell 1991; 64: 235–248.Google Scholar
11. Franza, BR jr, Sambucetti, LC, Cohen, DR, Curran, T: Analysis of Fos protein complex and Fos related antigens by high resolution two dimensional gel electrophoresis. Oncogene 1987; 1: 213–221.Google ScholarPubMed
12. Chiu, R, Boyle, JW, Meek, J, Smeal, T, Hunter, T, Karin, M: The c-Fos protein interacts with c-Jun/AP-1 to stimulate transcription of Ap-1 responsive genes. Cell 1988; 54: 541–552.Google Scholar
13. Goodrich, DW, Lee, WH: Abrogation by c-myc of G1 phase arrest induced by RB protein but not by p 53. Nature 1992; 360: 177–179.CrossRefGoogle ScholarPubMed