Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-11-24T12:36:12.728Z Has data issue: false hasContentIssue false
  • English
  • Français

Evaluation of Amniotic Fluid Elastolytic Activity: Can it be a Method of Fetal Lung Maturity Assessment? A Comparison with Gluck's L/S Test

Published online by Cambridge University Press:  01 August 2014

G. Bażowska ,
A. Jendryczko  and
J. Dudkiewicz
G. Bażowska
Affiliation:
2nd, Department of Obstetrics and Gynecology, Silesian School of Medicine
A. Jendryczko*
Affiliation:
Department of Drug Chemistry and Analysis, Silesian School of Medicine
J. Dudkiewicz
Affiliation:
2nd, Department of Obstetrics and Gynecology, Silesian School of Medicine
*
Kierownik Katedry i Zakladu, Chemii i Anallizy Leków, Śląskiej Akademii Medycznej, 41-200 Sosnowiec ul. Jagiellońska 4, Poland

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Amniotic fluid elastolytic activity was assessed in a group of 120 women who delivered preterm infants and in 35 women who delivered at term. Amniotic fluid elastolytic activity decreases as pregnancy progresses. The lecithin-to-sphingomyelin (L/S) ratio in women's amniotic fluid was determined by the method developed by Gluck and associates [6] and elastolytic activity by that developed by Mehdi and associates. A significant negative correlation was found between the amniotic fluid L/S ratio and amniotic fluid elastolytic activity (r = −0.932; p<0.001).

The border value of elastolytic activity that indicates lung maturity (L/S ratio equal to or greater than 2) is 2.01 ± 0.05 mmol/min ml. In the amniotic elastolytic activity test, it is the value that differentiates mature from immature lungs. The amniotic fluid elastolytic activity test is characterized by high sensitivity (91.43%) and specificity (91.67%), high positive prognostic value (76.19%) and low negative prognostic value (2.65%). The test parameters do not therefore differ greatly from those of the Gluck test. Moreover, the amniotic fluid elastolytic activity test is cheaper and takes less time to perform.

Keywords

Amniotic fluid elastolytic activityFetal lung maturity assessmentGluck's L/S testRespiratory distress syndrome
Type
Research Article
Information
Acta geneticae medicae et gemellologiae: twin research , Volume 43 , Issue 3-4 , October 1994 , pp. 185 - 192
Copyright
Copyright © The International Society for Twin Studies 1994

References

REFERENCES

1. Brown, LM, Duck-Chong, CG, Hensley, WJ (1982): Improved procedure for lecithin/sphingomyelin ratio in amniotic fluid reduces false prediction of lung immaturity. Clin Chem 28: 344–348.Google Scholar
2. Creasy, GW, Simon, N (1984): Sensitivity and specificity of the L/S ratio in relation to gestational age. Am J Perinatol 1: 302–309.Google Scholar
3. Cruz, AC, Buhi, WC, Birk, SA, Spellacy, WN (1976): Respiratory distress syndrome with mature lecithin/sphingomyelin ratios: diabetes mellitus and low Apgar scores. Am J Obstet Gynecol 126: 78–82.CrossRefGoogle ScholarPubMed
4. Desai, R, Wigglesworth, JS, Aber, V (1988): Assessment of elastin maturation by radioimmunoassay of desmosine in the developing human lung. Early Hum Dev 16: 61–71.Google Scholar
5. Garite, TJ, Freeman, RK, Nageotte, MP (1986): Fetal maturity cascade: a rapid and cost-effective method for fetal lung maturity testing. Obstet Gynecol 67: 619–622.Google Scholar
6. Gluck, L, Kulovich, MV, Borer, RC, Brenner, PH, Anderson, GG, Spellacy, WN (1971): Diagnosis of the respiratory distress syndrome by amniocentesis. Am J Obstet Gynecol 109: 440–445.Google Scholar
7. Hislop, AA, Wigglesworth, JS, Desai, R (1986): Alveolar development in the human fetus and infant. Early Hum Dev 13: 1–11.Google Scholar
8. Jobe, AH (1991): Pathogenesis of respiratory failure in the preterm infant. Ann Med 23: 687–691.Google Scholar
9. Kelly, FW, Fagan, DG, Webster, SI (1977): Quantity and character of elastin in developing lung parenchymal tissues of normal infants and infants with respiratory distress syndrome. J Lab Clin Med 90: 981–989.Google Scholar
10. Mehdi, S, Angelastro, MR, Burkhart, JP, Koehl, JR, Peet, NP, Bey, P (1990): The inhibition of human neutrophil elastase and catepsin G by peptidyl 1, 2-dicarbonyl derivatives. Biochem Biophys Res Commun 166: 595–603.CrossRefGoogle Scholar
11. Rucker, RB, Dubick, MA (1984): Elastin metabolism and chemistry: potential roles in lung development and structure. Environ Health Perspect 55: 179–191.Google Scholar
12. Skinner, SJ, Campos, GA, Liggins, GC (1981): Collagen content of human amniotic membranes: effect of gestation length and premature ruptures. Obstet Gynecol 57: 487–489.Google Scholar
13. Statlend, BE, Sher, G (1985): Reliability of amniotic fluid surfactant measurements. Am J Clin Pathol, 83: 382–384.CrossRefGoogle Scholar