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An Analysis of Several Slide Preparation Techniques for Human Peripheral Blood Cultures

Published online by Cambridge University Press:  01 August 2014

Edmund C. Jenkins  and
Richard G. Weed
Edmund C. Jenkins*
Affiliation:
The Cytogenetics Laboratory, New York State Institute for Basic Research in Mental Retardation, Staten Island, New York
Richard G. Weed
Affiliation:
The Cytogenetics Laboratory, New York State Institute for Basic Research in Mental Retardation, Staten Island, New York
*
1050 Forest Hill Road, Staten Island, New York 10314, USA

Summary

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Several slide preparation procedures were studied in order to render a semiquantitative indication of an optimum technique or techniques, concerning the over-all cytological quality of the chromosome spreads effected by each technique. A method of scoring for karyotypically analyzable spreads is given. It is concluded that one of the techniques (technique D) be utilized for routine karyotypic studies while a combination of two of the techniques (techniques D and E) should be employed for more extensive studies.

Type
Research Article
Information
Acta geneticae medicae et gemellologiae: twin research , Volume 20 , Issue 1 , January 1971 , pp. 85 - 95
Copyright
Copyright © The International Society for Twin Studies 1971

References

Difco Supplementary Literature (1968). TC - Chromosome Microtest Kit. Difco Laboratories, Detroit.Google Scholar
Gibco Price and Reference Manual (1967). Chromosome Medium 1A. Grand Island Biological Company, Grand Island, N. Y. Google Scholar
Hungerford, D. A. (1965). Leukocytes cultured from small inocula of whole blood and the preparation of metaphase chromosomes by treatment with hypotonic KC1. Stain Techn., 40: 333–338.CrossRefGoogle Scholar
Merchant, D. J., Kahn, R. Y., Murphy, W. H. Jr. (1964). Enumeration of Chromosomes. In: Handbook of Cell and Organ Culture. Burgess Publ., Minneapolis.Google Scholar
Schera, R. G. (1962). Blaze drying by igniting the fixative for improved spreads of chromosomes in leucocytes. Stain Techn., 37: 386.CrossRefGoogle Scholar
Turpin, R., Lejeune, J. (1965). Les Chromosomes Humains. Gauthier-Villars, Paris.Google Scholar