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Dietary magnesium deficiency affects gut microbiota and anxiety-like behaviour in C57BL/6N mice

Published online by Cambridge University Press:  16 March 2015

Bettina Pyndt Jørgensen ,
Gudrun Winther ,
Pernille Kihl ,
Dennis S. Nielsen ,
Gregers Wegener ,
Axel K. Hansen  and
Dorte B. Sørensen
Bettina Pyndt Jørgensen*
Affiliation:
Section of Experimental Animal Models, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C, Denmark
Gudrun Winther
Affiliation:
Translational Neuropsychiatry Unit, Department of Clinical Medicine, Aarhus University, Risskov, Denmark
Pernille Kihl
Affiliation:
Section of Experimental Animal Models, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C, Denmark
Dennis S. Nielsen
Affiliation:
Department of Food Science, Faculty of Science, University of Copenhagen, Frederiksberg C, Denmark
Gregers Wegener
Affiliation:
Translational Neuropsychiatry Unit, Department of Clinical Medicine, Aarhus University, Risskov, Denmark Pharmaceutical Centre of Excellence, School of Pharmacy, North West University, Potchefstroom, South Africa
Axel K. Hansen
Affiliation:
Section of Experimental Animal Models, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C, Denmark
Dorte B. Sørensen
Affiliation:
Section of Experimental Animal Models, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C, Denmark
*
Bettina Pyndt Jørgensen, Section of Experimental Animal Models, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Thorvaldsensvej 57, 1871 Frederiksberg C, Denmark. Tel: +453 533 2724; Fax: +453 533 2755; E-mail: [email protected]
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Abstract

Objective

Magnesium deficiency has been associated with anxiety in humans, and rodent studies have demonstrated the gut microbiota to impact behaviour.

Methods

We investigated the impact of 6 weeks of dietary magnesium deficiency on gut microbiota composition and anxiety-like behaviour and whether there was a link between the two. A total of 20 C57BL/6 mice, fed either a standard diet or a magnesium-deficient diet for 6 weeks, were tested using the light-dark box anxiety test. Gut microbiota composition was analysed by denaturation gradient gel electrophoresis.

Results

We demonstrated that the gut microbiota composition correlated significantly with the behaviour of dietary unchallenged mice. A magnesium-deficient diet altered the gut microbiota, and was associated with altered anxiety-like behaviour, measured by decreased latency to enter the light box.

Conclusion

Magnesium deficiency altered behavior. The duration of magnesium deficiency is suggested to influence behaviour in the evaluated test.

Keywords

animal modelsanxietygut microbiotamagnesium
Type
Short Communications
Information
Acta Neuropsychiatrica , Volume 27 , Issue 5 , October 2015 , pp. 307 - 311
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Copyright
© Scandinavian College of Neuropsychopharmacology 2015 

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References

1. Eby, GA, Eby, KL. Rapid recovery from major depression using magnesium treatment. Med Hypotheses 2006;67:362–370.CrossRefGoogle ScholarPubMed
2. Eby, GA 3rd, Eby, KL. Magnesium for treatment-resistant depression: a review and hypothesis. Med Hypotheses 2010;74:649–660.CrossRefGoogle ScholarPubMed
3. Singewald, N, Sinner, C, Hetzenauer, A et al. Magnesium-deficient diet alters depression- and anxiety-related behavior in mice – influence of desipramine and Hypericum perforatum extract. Neuropharmacology 2004;47:1189–1197.Google Scholar
4. Sartori, SB, Whittle, N, Hetzenauer, A et al. Magnesium deficiency induces anxiety and HPA axis dysregulation: modulation by therapeutic drug treatment. Neuropharmacology 2012;62:304–312.CrossRefGoogle ScholarPubMed
5. Faravelli, C, Lo Sauro, C, Godini, L et al. Childhood stressful events, HPA axis and anxiety disorders. World J Psychiatry 2012;2:13–25.CrossRefGoogle ScholarPubMed
6. Bangsgaard Bendtsen, KM, Krych, L, Sørensen, DB et al. Gut microbiota composition is correlated to grid floor induced stress and behavior in the BALB/c mouse. PLoS One 2012;7:46231.CrossRefGoogle ScholarPubMed
7. Bercik, P, Denou, E, Collins, J et al. The intestinal microbiota affect central levels of brain-derived neurotrophic factor and behavior in mice. Gastroenterology 2011;141:599–U701.Google Scholar
8. Collins, SM, Kassam, Z, Bercik, P. The adoptive transfer of behavioral phenotype via the intestinal microbiota: experimental evidence and clinical implications. Curr Opin Microbiol 2013;16:240–245.CrossRefGoogle ScholarPubMed
9. Sudo, N, Chida, Y, Aiba, Y et al. Postnatal microbial colonization programs the hypothalamic–pituitary–adrenal system for stress response in mice. J Physiol (Lond) 2004;558:263–275.CrossRefGoogle ScholarPubMed
10. Heijtz, RD, Wang, S, Anuar, F et al. Normal gut microbiota modulates brain development and behavior. Proc Nat Acad Sci 2011;108:3047–3052.CrossRefGoogle Scholar
11. Neufeld, KA, Kang, N, Bienenstock, J et al. Effects of intestinal microbiota on anxiety-like behavior. Commun Integr Biol 2011;4:492–494.Google Scholar
12. Zimowska, W, Girardeau, JP, Kuryszko, J et al. Morphological and immune response alterations in the intestinal mucosa of the mouse after short periods on a low-magnesium diet. Br J Nutr 2002;88:515–522.Google Scholar
13. Crawley, JN. Neuropharmacologic specificity of a simple animal model for the behavioral actions of benzodiazepines. Pharmacol Biochem Behav 1981;15:695–699.Google Scholar
14. Pyndt Jørgensen, B, Hansen, JT, Krych, L et al. A possible link between food and mood: dietary impact on gut microbiota and behavior in BALB/c Mice. PLoS One 2014;9:e103398.Google Scholar
15. Lundberg, R, Clausen, SK, Pang, W et al. Gastrointestinal microbiota and local inflammation during oxazolone-induced dermatitis in BALB/cA mice. Comp Med 2012;62:371–380.Google Scholar
16. Muyzer, G, Smalla, K. Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology. Antonie Van Leeuwenhoek 1998;73:127–141.Google Scholar
17. Winther, G. Magnesium deficiency induces anxiety- and depression-like behavior and metabolic dysfunction in C57Bl/6 J mice, MSc Thesis, University of Aarhus, Denmark. 2012.Google Scholar
18. Poleszak, E, Szewczyk, B, Kędzierska, E et al. Antidepressant- and anxiolytic-like activity of magnesium in mice. Pharmacol Biochem Behav 2004;78:7–12.Google Scholar
19. Pachikian, BD, Neyrinck, AM, Deldicque, L et al. Changes in intestinal Bifidobacteria levels are associated with the inflammatory response in magnesium-deficient mice. J Nutr 2010;140:509–514.Google Scholar
20. Collins, S, Verdu, E, Denou, E et al. The role of pathogenic microbes and commensal bacteria in irritable bowel syndrome. (IBD and IBS: Novel mechanisms and future practice.). Dig Dis 2009;1:85–89.CrossRefGoogle Scholar