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No borna disease virus-specific RNA detected in blood of race horses and jockeys

Published online by Cambridge University Press:  24 June 2014

Yong-Ku Kim ,
Kyung-Bo Noh ,
Chang-Su Han ,
Ju-Young Moon ,
Do-Kyung Yoon ,
Ki-Joon Song ,
Dai-Jin Kim ,
Marta Kubera ,
Michael Maes  and
Jin-Won Song
Yong-Ku Kim*
Affiliation:
Department of Psychiatry, College of Medicine, Korea University, Seoul, Korea
Kyung-Bo Noh
Affiliation:
Department of Microbiology and Institute for Viral Diseases, College of Medicine, Korea University, Seoul, Korea
Chang-Su Han
Affiliation:
Department of Psychiatry, College of Medicine, Korea University, Seoul, Korea
Ju-Young Moon
Affiliation:
Department of Microbiology and Institute for Viral Diseases, College of Medicine, Korea University, Seoul, Korea
Do-Kyung Yoon
Affiliation:
Department of Family Medicine, College of Medicine, Korea University, Seoul, Korea
Ki-Joon Song
Affiliation:
Department of Microbiology and Institute for Viral Diseases, College of Medicine, Korea University, Seoul, Korea
Dai-Jin Kim
Affiliation:
Department of Psychiatry, College of Medicine, The Catholic University, Seoul, Korea
Marta Kubera
Affiliation:
Department of Endocrinology, Institute of Pharmacology, Polish Academy of sciences, Krakow, Poland
Michael Maes
Affiliation:
MCare4U Outpatient Clinics, Lier, Belgium
Jin-Won Song
Affiliation:
Department of Microbiology and Institute for Viral Diseases, College of Medicine, Korea University, Seoul, Korea
*
Professor Yong-Ku Kim, Department of Psychiatry, Korea University Ansan Hospital, 516, Gojan Dong, Danwon Gu, Ansan city, Kyunggi Province 425-020, South Korea. Tel: +82 31 412 5140; Fax: +82 31 412 5144; E-mail: [email protected]
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Abstract

Background:

Borna disease virus (BDV) predominantly infects horses and sheep, causing a broad range of behavioural disorders. It is controversial whether BDV infects humans and causes psychiatric disorders.

Objectives:

We searched for BDV-derived nucleic acids in blood of race horses and jockeys riding the horses.

Methods:

We assayed for the BDV genome in RNA extracted from peripheral blood mononuclear cells (PBMC) of 39 race horses and 48 jockeys. Two polymerase chain reaction protocols [one-tube reverse transcription–polymerase chain reaction (RT-PCR) and two-step RT-PCR] were used to assay BDV p24 and p40 transcripts.

Results:

The p24 and p40 viral nucleic acid sequences were not detected in the PBMC RNAs from any of the race horses or jockeys.

Conclusions:

These data do not support an epidemiological association between BDV infection, race horses and humans.

Keywords

BDVhorsejockeyPCRpsychiatric disorder
Type
Brief Report
Information
Acta Neuropsychiatrica , Volume 18 , Issue 3-4 , June 2006 , pp. 177 - 180
Copyright
Copyright © 2006 Blackwell Munksgaard

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