Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-24T12:12:57.717Z Has data issue: false hasContentIssue false
  • English
  • Français

Failure to detect the action of antidepressants in the forced swim test in Swiss mice

Published online by Cambridge University Press:  05 December 2017

Patrick R. Suman ,
Nathalia Zerbinatti ,
Lais Cristina Theindl ,
Karolina Domingues  and
Cilene Lino de Oliveira
Patrick R. Suman
Affiliation:
Department of Physiological Sciences, Center of Biological Sciences, Federal University of Santa Catarina – UFSC, Campus Trindade, 88040-900 Florianópolis, SC, Brazil Department of Pharmacology, Center of Biological Sciences, Federal University of Santa Catarina – UFSC, Campus Trindade, 88040-900 Florianópolis, SC, Brazil
Nathalia Zerbinatti
Affiliation:
Department of Physiological Sciences, Center of Biological Sciences, Federal University of Santa Catarina – UFSC, Campus Trindade, 88040-900 Florianópolis, SC, Brazil Department of Pharmacology, Center of Biological Sciences, Federal University of Santa Catarina – UFSC, Campus Trindade, 88040-900 Florianópolis, SC, Brazil
Lais Cristina Theindl
Affiliation:
Department of Physiological Sciences, Center of Biological Sciences, Federal University of Santa Catarina – UFSC, Campus Trindade, 88040-900 Florianópolis, SC, Brazil Department of Pharmacology, Center of Biological Sciences, Federal University of Santa Catarina – UFSC, Campus Trindade, 88040-900 Florianópolis, SC, Brazil
Karolina Domingues
Affiliation:
Department of Physiological Sciences, Center of Biological Sciences, Federal University of Santa Catarina – UFSC, Campus Trindade, 88040-900 Florianópolis, SC, Brazil Department of Pharmacology, Center of Biological Sciences, Federal University of Santa Catarina – UFSC, Campus Trindade, 88040-900 Florianópolis, SC, Brazil
Cilene Lino de Oliveira*
Affiliation:
Department of Physiological Sciences, Center of Biological Sciences, Federal University of Santa Catarina – UFSC, Campus Trindade, 88040-900 Florianópolis, SC, Brazil Department of Pharmacology, Center of Biological Sciences, Federal University of Santa Catarina – UFSC, Campus Trindade, 88040-900 Florianópolis, SC, Brazil
*
Dr. Cilene Lino de Oliveira. Departamento de Ciências Fisiológicas, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário Trindade, 88049-900 Florianópolis, SC, Brazil. Tel: +55 48 3721 4689; Fax: +55 48 3721 9672; E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Objective

The aims of this study were to replicate previously published experiments and to modify the protocol to detect the effects of chronic antidepressant treatment in mice.

Methods

Male Swiss mice (n=6–8/group) housed in reversed light/dark cycle were randomly assigned into receive vehicle (10% sucrose), sub-effective doses (1 and 3 mg/kg) or effective doses (10 and 30 mg/kg) of bupropion, desipramine, and fluoxetine and a candidate antidepressant, sodium butyrate (1–30 mg/kg) per gavage (p.o.) 1 h before the forced swim test (FST). Treatments continued daily for 7 and 14 days during retests 1 and 2, respectively. In an additional experiment, mice received fluoxetine (20 mg/kg) or vehicle (10% sucrose or 0.9% saline) p.o. or i.p. before the FST. Mice housed in reversed or standard light/dark cycles received fluoxetine (20 mg/kg) prior FST. Video recordings of behavioural testing were used for blind assessment of the outcomes.

Results

According to the expected, doses of antidepressants considered sub-effective failed to affect the immobility time of mice in the FST. Surprisingly, acute and chronic treatment with the high doses of bupropion, desipramine, and fluoxetine or sodium butyrate also failed to reduce the immobility time of mice in the FST. Fluoxetine 20 mg/kg was also ineffective in the FST when injected i.p. or in mice housed in normal light/dark cycle.

Conclusion

Data suggest the lack of efficacy of orally administered bupropion, desipramine, fluoxetine in the FST in Swiss mice. High variability, due to high and low immobility mice, may explain the limited effects of the treatments.

Keywords

animal experimentationantidepressive agentsdrugspsychopharmacology
Type
Original Article
Information
Acta Neuropsychiatrica , Volume 30 , Issue 3 , June 2018 , pp. 158 - 167
Copyright
© Scandinavian College of Neuropsychopharmacology 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Belzung, C. Innovative drugs to treat depression: did animal models fail to be predictive or did clinical trials fail to detect effects? Neuropsychopharmacology 2014;39:1041–1051.Google Scholar
2. Millan, MJ, Goodwin, GM, Meyer-Lindenberg, A, Ove Ogren, S. Learning from the past and looking to the future: emerging perspectives for improving the treatment of psychiatric disorders. Eur Neuropsychopharmacol 2015;25:599–656.Google Scholar
3. Berton, O, Hahn, CG, Thase, ME. Are we getting closer to valid translational models for major depression? Science 2012;338:75–79.Google Scholar
4. Bourin, M. New challenges for translational psychopharmacology. Front Psychiatry 2010;1:3.Google Scholar
5. Porsolt, RD, Bertin, A, Jalfre, M. “Behavioural despair” in rats and mice: strain differences and the effects of imipramine. Eur J Pharmacol 1978;51:291–294.Google Scholar
6. Bourin, M, Chenu, F, Ripoll, N, David, DJ. A proposal of decision tree to screen putative antidepressants using forced swim and tail suspension tests. Behav Brain Res 2005;164:266–269.Google Scholar
7. Lucki, I, Dalvi, A, Mayorga, A. Sensitivity to the effects of pharmacologically selective antidepressants in different strains of mice. Psychopharmacology 2001;155:315–322.Google Scholar
8. Petit-Demouliere, B, Chenu, F, Bourin, M. Forced swimming test in mice: a review of antidepressant activity. Psychopharmacology (Berl) 2005;177:245–255.Google Scholar
9. Costa, AP, Vieira, C, Bohner, LO et al. A proposal for refining the forced swim test in Swiss mice. Prog Neuropsychopharmacol Biol Psychiatry 2013;45:150–155.Google Scholar
10. Castagne, V, Moser, P, Porsolt, RD. Behavioral assessment of antidepressant activity in rodents. In: Buccafusco JJ, editor. Methods of Behavior Analysis in Neuroscience, CRC Press, Boca Raton; 2009; 103–117.Google Scholar
11. Mezadri, TJ, Batista, GM, Portes, AC, Marino-Neto, J, Lino-de-Oliveira, C. Repeated rat-forced swim test: reducing the number of animals to evaluate gradual effects of antidepressants. J Neurosci Methods 2011;195:200–205.Google Scholar
12. Possamai, F, dos Santos, J, Walber, T, Marcon, JC, dos Santos, TS, Lino de Oliveira, C. Influence of enrichment on behavioral and neurogenic effects of antidepressants in Wistar rats submitted to repeated forced swim test. Prog Neuropsychopharmacol Biol Psychiatry 2015;58:15–21.Google Scholar
13. David, DJ, Renard, CE, Jolliet, P, Hascoet, M, Bourin, M. Antidepressant-like effects in various mice strains in the forced swimming test. Psychopharmacology (Berl) 2003;166:373–382.Google Scholar
14. Borsini, F. Role of the serotonergic system in the forced swimming test. Neurosci Biobehav Rev 1995;19:377–395.Google Scholar
15. Lucki, I, Singh, A, Kreiss, DS. Antidepressant-like behavioral effects of serotonin receptor agonists. Neurosci Biobehav Rev 1994;18:85–95.Google Scholar
16. Borsini, F, Meli, A. Is the forced swimming test a suitable model for revealing antidepressant activity? Psychopharmacology (Berl) 1988;94:147–160.Google Scholar
17. Bourin, M, Colombel, MC, Redrobe, JP, Nizard, J, Hascoet, M, Baker, GB. Evaluation of efficacies of different classes of antidepressants in the forced swimming test in mice at different ages. Prog Neuropsychopharmacol Biol Psychiatry 1998;22:343–351.Google Scholar
18. Dulawa, SC, Holick, KA, Gundersen, B, Hen, R. Effects of chronic fluoxetine in animal models of anxiety and depression. Neuropsychopharmacology 2004;29:1321–1330.Google Scholar
19. Jesse, CR, Wilhelm, EA, Nogueira, CW. Depression-like behavior and mechanical allodynia are reduced by bis selenide treatment in mice with chronic constriction injury: a comparison with fluoxetine, amitriptyline, and bupropion. Psychopharmacology (Berl) 2010;212:513–522.Google Scholar
20. Lenzi, J, Rodrigues, AF, Ros Ade, S et al. Ferulic acid chronic treatment exerts antidepressant-like effect: role of antioxidant defense system. Metab Brain Dis 2015;30:1453–1463.Google Scholar
21. Oh, JE, Zupan, B, Gross, S, Toth, M. Paradoxical anxiogenic response of juvenile mice to fluoxetine. Neuropsychopharmacology 2009;34:2197–2207.Google Scholar
22. Junior, CFC, Pederiva, CN, Bose, RC, Garcia, VA, Lino-de-Oliveira, C, Marino-Neto, J. ETHOWATCHER: validation of a tool for behavioral and video-tracking analysis in laboratory animals. Comput Biol Med 2012;42:257–264.Google Scholar
23. Crispim Junior, CF, Pederiva, CN, Bose, RC, Garcia, VA, Lino-de-Oliveira, C, Marino-Neto, J. ETHOWATCHER: validation of a tool for behavioral and video-tracking analysis in laboratory animals. Comput Biol Med 2012;42:257–264.Google Scholar
24. Bogdanova, OV, Kanekar, S, D’Anci, KE, Renshaw, PF. Factors influencing behavior in the forced swim test. Physiol Behav 2013;118:227–239.Google Scholar
25. Enriquez-Castillo, A, Alamilla, J, Barral, J et al. Differential effects of caffeine on the antidepressant-like effect of amitriptyline in female rat subpopulations with low and high immobility in the forced swimming test. Physiol Behav 2008;94:501–509.Google Scholar
26. Flores-Serrano, AG, Vila-Luna, ML, Alvarez-Cervera, FJ, Heredia-Lopez, FJ, Gongora-Alfaro, JL, Pineda, JC. Clinical doses of citalopram or reboxetine differentially modulate passive and active behaviors of female Wistar rats with high or low immobility time in the forced swimming test. Pharmacol Biochem Behav 2013;110:89–97.Google Scholar
27. Nascimento, FP, Macedo-Junior, SJ, Borges, FR et al. Thalidomide reduces mechanical hyperalgesia and depressive-like behavior induced by peripheral nerve crush in mice. Neuroscience 2015;303:51–58.Google Scholar
28. Bhatt, S, Mahesh, R, Jindal, A, Devadoss, T. Neuropharmacological effect of novel 5-HT3 receptor antagonist, N-n-propyl-3-ethoxyquinoxaline-2-carboxamide (6n) on chronic unpredictable mild stress-induced molecular and cellular response: Behavioural and biochemical evidences. Pharmacol Rep 2014;66:804–810.Google Scholar
29. Pawar, GR, Agrawal, RP, Phadnis, P, Paliwal, A, Vyas, S, Solanki, P. Evaluation of antidepressant like property of amisulpride per se and its comparison with fluoxetine and olanzapine using forced swimming test in albino mice. Acta Pol Pharm 2009;66:327–331.Google Scholar
30. Pandey, DK, Rajkumar, R, Mahesh, R, Radha, R. Depressant-like effects of parthenolide in a rodent behavioural antidepressant test battery. J Pharm Pharmacol 2008;60:1643–1650.Google Scholar
31. Ren, LX, Luo, YF, Li, X, Zuo, DY, Wu, YL. Antidepressant-like effects of sarsasapogenin from Anemarrhena asphodeloides BUNGE (Liliaceae). Biol Pharm Bull 2006;29:2304–2306.Google Scholar
32. El Yacoubi, M, Bouali, S, Popa, D et al. Behavioral, neurochemical, and electrophysiological characterization of a genetic mouse model of depression. Proc Natl Acad Sci USA 2003;100:6227–6232.Google Scholar
33. Jayatissa, MN, Bisgaard, C, Tingstrom, A, Papp, M, Wiborg, O. Hippocampal cytogenesis correlates to escitalopram-mediated recovery in a chronic mild stress rat model of depression. Neuropsychopharmacology 2006;31:2395–2404.Google Scholar
34. Caldarone, BJ, Zachariou, V, King, SL. Rodent models of treatment-resistant depression. Eur J Pharmacol 2015;753:51–65.Google Scholar
35. Willner, P, Belzung, C. Treatment-resistant depression: are animal models of depression fit for purpose? Psychopharmacology (Berl) 2015;232:3473–3495.Google Scholar
36. Covington, HE III, Maze, I, LaPlant, QC et al. Antidepressant actions of histone deacetylase inhibitors. J Neurosci 2009;29:11451–11460.Google Scholar
37. Schroeder, FA, Lin, CL, Crusio, WE, Akbarian, S. Antidepressant-like effects of the histone deacetylase inhibitor, sodium butyrate, in the mouse. Biol Psychiatry 2007;62:55–64.Google Scholar
38. Gundersen, BB, Blendy, JA. Effects of the histone deacetylase inhibitor sodium butyrate in models of depression and anxiety. Neuropharmacology 2009;57:67–74.Google Scholar
39. Covington, HE III, Vialou, VF, LaPlant, Q, Ohnishi, YN, Nestler, EJ. Hippocampal-dependent antidepressant-like activity of histone deacetylase inhibition. Neurosci Lett 2011;493:122–126.Google Scholar
40. Han, A, Sung, YB, Chung, SY, Kwon, MS. Possible additional antidepressant-like mechanism of sodium butyrate: targeting the hippocampus. Neuropharmacology 2014;81:292–302.Google Scholar
41. Qiu, X, Xiao, X, Li, N, Li, Y. Histone deacetylases inhibitors (HDACis) as novel therapeutic application in various clinical diseases. Prog Neuropsychopharmacol Biol Psychiatry 2017;72:60–72.Google Scholar
Supplementary material: File

Suman et al supplementary material 1

Suman et al supplementary material

Download Suman et al supplementary material 1(File)
File 102.1 KB