Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
Hostname: page-component-586b7cd67f-r5fsc Total loading time: 0 Render date: 2024-11-24T11:41:56.812Z Has data issue: false hasContentIssue false

Chapter 32 - Molecular and cellular integrity of cultured follicles

from Section 8 - In vitro follicle growth and maturation

Published online by Cambridge University Press:  04 February 2011

By
David F. Albertini ,
Gokhan Akkoyunlu  and
S. Samuel Kim
Edited by
Jacques Donnez  and
S. Samuel Kim
Jacques Donnez
Affiliation:
Université Catholique de Louvain, Belgium
S. Samuel Kim
Affiliation:
University of Kansas
Get access

Summary

This chapter reviews methods that have been in practice for the evaluation of follicle culture integrity and points out the strengths and deficiencies of these methods. The traditional array of biomarkers for the ovarian follicle has emphasized endocrine performance, especially as related to the biosynthesis and secretion of estradiol. The link between DNA damage sensing and repair is fast becoming a major determinant in the assessment of follicle integrity as it relates to both somatic and germ cell components, and these assays have introduced a range of sensitivity and precision that will materially advance the field of follicle culture. The evaluation of follicle integrity for future applications in fertility preservation requires changes in our conceptual and technological approaches to this problem. The emphasis dictated by the need to obtain healthy oocytes of high developmental competence warrants conceptualizing folliculogenesis as an oocentric process.

Keywords

cervical cancerconcurrent chemotherapyDargent's operationlaparotomylaparoscopyfertility-saving surgerylaparoscopic pelvic lymph-noderadical trachelectomyradiotherapy
Type
Chapter
Information
Principles and Practice of Fertility Preservation , pp. 389 - 396
Publisher: Cambridge University Press
Print publication year: 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Save book to Kindle

To save this book to your Kindle, first ensure [email protected] is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×