Appendix 2 - Laboratory Safety

Summary

Due to the wide variety of experiments listed in this text it would be cumbersome to roster each specific chemical danger here. Therefore, dangers inherent to each exercise are listed in the experiments in each chapter. The following safety precautions are a generalized list that are likely to be encountered in the exercises covered in the text. Individuals involved in the biochemical experimentation should familiarize themselves intimately with these precautions.

General Precautions

• 1. Follow the instructions and pay attention to all the steps from start to finish before beginning an experiment. Know the use of all the equipment in the lab before beginning the work.

• 2. The most important safety rule is to know the location of the safety equipment and how to use it. The equipment should be checked periodically to ensure that it is in working order. Remember the building evacuation protocol.

• 3. Wear safety goggles at all times in the laboratory. For biochemical experimentation regular eyeglasses do not provide sufficient protection from either chemical hazards or broken glassware. Recently, the American Chemical Society has approved the use of contact lenses but only when worn in combination with safety goggles. The contact/goggle combination is important because most modern eyewash stations are not able to remove chemicals trapped behind contacts.

• 4. Proper clothing should be worn in the laboratory at all times. Long sleeve shirts, long pants and full shoes are the best choice. Skin protection will be at a maximum if the aforementioned garments are covered with a full lab coat/apron.

• 5. Make use of the fume hoods for handling volatile and/or hazardous chemicals. When handling chemicals gloves should be worn to protect the hands.

• 6. Dispose of solid and liquid waste in containers, which are properly labeled. Notify the instructor if any of the waste containers are full or damaged. If you not sure where to dispose of the waste ask your instructor for help.

• 7. Get acquainted with the layout of the lab, paying special attention to the fire extinguishers, emergency eye wash stations, first aid kits and the nearest emergency phone. Knowledge of the location of this apparatus may help save your life and prevent injury to others.

• 8. Never eat or drink in the laboratory area. Contamination of food or drink can take place without your knowledge.